In the Assign Samples to Wells page, set up each well as follows.
In the Sample ID field, select Select Sample,
In the Select a Biological Sample dialog box, select a sample, and then select Select.
In the Library ID field, enter a unique name for the library.
Select the Index drop-down arrow, and select a unique index to add to the sample.
If you selected mixed insert sizes on the previous page, select the Insert Size drop-down arrow, and select the insert size of the library.
Select Next.