The following examples show possible yield scenarios:
Example 1: Required Yield Met — The lab produces the required yield specified in the biosample workflow file.
Example 2: Missing Yield — The lab does not produce all of the required yield. A lab requeue is initated and fulfilled.
Example 3: Missing Yield After Lab Requeue — The lab does not produce the required yield from the initial request or the lab requeue. A second lab requeue is initiated and fulfilled.
In this example, the original request and lab requeue do not produce the required yield before the yield timeout, requiring a second lab requeue.
A biosample manifest is imported with a prep request required yield of 105 Gbp.
The lab produces 80 Gbp of QC-passed FASTQ data sets. Actual yield is increased and the amount still pending is changed to 15 GBP to reflect the remaining balance. The expected yield does not change because it represents the total amount expected from the lab and includes actual yield and pending yield.
Yield timeout is reached with no additional yield received.
The default yield timeout is four days, however you can configure a different timeout period using the BaseSpace Sequence Hub API. For more information, see the developer documentation at developer.basespace.illumina.com.
When the yield timeout is reached, BaseSpace Sequence Hub assumes that the lab is unable to produce the requested quantity. The amount pending is classified as missing, and the expected yield is reduced to the actual yield received, indicating that no additional data are expected from the lab.
(table scrolls to the right...)
A lab requeue for more yield is initiated. In this example, the user requested more than the missing amount.
Expected yield updates. The updated amount reflects the quantity received from the initial request and the additional amount requested from the lab requeue. The required yield does not change because that is the amount of data required for analysis.
Missing yield is reset to zero.
Requested yield updates on the Requeues tab.
(table scrolls to the right...)
The lab produces 20 Gbp of QC-passed FASTQ data sets. Actual yield is increased and the amount still pending is changed to 10 GBP to reflect the remaining balance. The expected yield does not change because it represents the total amount expected from the lab and includes actual yield and pending yield.
(table scrolls to the right...)
The lab requeue expires after the timeout period. As with the yield timeout, the amount pending is classified as missing and the expected yield is reduced to the actual yield received, indicating that no additional data are expected from the lab.
(table scrolls to the right...)
Another lab requeue is initiated for 10 Mbp. The new request is listed on the biosample Requeues tab.
(table scrolls to the right...)
The lab produces 5 Mbp of QC-passed FASTQ data. The lab requeue is not completely fulfilled but the required yield dependency is met and analyses can automatically launch if they are not waiting for other dependencies.
(table scrolls to the right...)
A biosample manifest is uploaded with a prep request required yield of 105 Gbp.
80 Gbp of QC-passed FASTQ data sets are received. The actual yield is increased and the amount still pending is changed to 25 GBP to reflect the remaining balance. The expected yield does not change because it represents the total amount expected from the lab and includes actual yield and pending yield.
The remaining 25 Gbp of FASTQ data sets are received. Actual yield is updated with the received amount. The full amount expected from the lab has been received, so there is no more pending yield.
In this example, the full amount of required yield is not received before the yield timeout, requiring a lab requeue to request additional sequencing data
A biosample manifest is imported with a prep request required yield of 105 Gbp.
80 Gbp of QC-passed FASTQ data sets are received. The actual yield is increased and the amount still pending is changed to 15 GBP to reflect the remaining balance. The expected yield does not change because it represents the total amount expected from the lab and includes actual yield and pending yield.
Yield timeout is reached with no additional yield received.
The default yield timeout is four days, however you can configure a different timeout period using the Basespace Sequence Hub API. For more information, see the developer documentation at .
When the yield timeout is reached, BaseSpace Sequence Hub assumes that the lab is unable to produce the requested quantity. The amount pending is classified as missing, and the expected yield is reduced to the actual yield received, indicating that no additional data are expected from the lab.
__
(table scrolls to the right)
A lab requeue for more yield is initiated. In this example, the user requests more than the missing amount.
Expected yield updates. The updated amount reflects the quantity received from the initial request and the additional amount requested from the lab requeue. The required yield does not change because that is the amount of data required for analysis.
Missing yield is reset to zero.
Requested yield updates on the Requeues tab.
(table scrolls to the right)
The lab produces 47 Gbp of QC-passed FASTQ data sets. Actual and expected yield are updated to reflect the total amount received from the lab.
(table scrolls to the right)
Required
Expected
Actual
Pending
3a: Yield Requested
+105
+105
0
+105
Total
105
105
0
105
Required
Expected
Actual
Pending
3a: Yield Requested
+105
+105
0
+105
3b: Partial Yield Received
+80
–80
Total
105
105
80
25
Required
Expected
Actual
Pending
Missing
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
Total
105
80
80
0
25
Required
Expected
Actual
Pending
Missing
Requested
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
3d: Lab Requeue for Additional Yield
+30
+30
–25
+30
Total
105
110
80
30
0
30
Required
Expected
Actual
Pending
Missing
Requested
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
3d: Lab Requeue for Additional Yield
+30
+30
–25
+30
3e: Partial Lab Requeue Data Received
+20
–20
Total
105
110
100
10
0
30
Required
Expected
Actual
Pending
Missing
Requested
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
3d: Lab Requeue for Additional Yield
+30
+30
–25
+30
3e: Partial Lab Requeue Data Received
+20
–20
3f: Lab Requeue Expires
–10
–10
+10
Total
105
100
100
0
10
30
Required
Expected
Actual
Pending
Missing
Requested
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
3d: Lab Requeue for Additional Yield
+30
+30
–25
+30
3e: Partial Lab Requeue Data Received
+20
–20
3f: Lab Requeue Expires
–10
–10
+10
3g: New Lab Requeue
+10
+10
–10
+10
Total
105
110
100
10
0
40
Required
Expected
Actual
Pending
Missing
Requested
3a: Yield Requested
+105
+105
0
+105
0
3b: Partial Yield Received
+80
–80
0
3c: Yield Timeout
-25
–25
+25
3d: Lab Requeue for Additional Yield
+30
+30
–25
+30
3e: Partial Lab Requeue Data Received
+20
–20
3f: Lab Requeue Expires
–10
–10
+10
3g: New Lab Requeue
+10
+10
–10
+10
3h: Lab Requeue Data Received
+5
–5
–10
Total
105
110
105
5
0
40
Required | Expected | Actual | Pending |
1a: Yield Requested | +105 | +105 | 0 | +105 |
Total | 105 | 105 | 0 | 105 |
Required | Expected | Actual | Pending |
1a: Yield Requested | +105 | +105 | 0 | +105 |
1b: Partial Yield Received | +80 | –80 |
Total | 105 | 105 | 80 | 25 |
Required | Expected | Actual | Pending |
1a: Yield Requested | +105 | +105 | 0 | +105 |
1b: Partial Yield Received | +80 | –80 |
1c: Balance of Yield Received | +25 | –25 |
Total | 105 | 105 | 105 |
Required | Expected | Actual | Pending | Missing |
2a: Yield Requested | +105 | +105 | 0 | +105 | 0 |
2b: Partial Yield Received | +80 | –80 | 0 |
2c: Yield Timeout | -25 | –25 | +25 |
Total | 105 | 80 | 80 | 0 | 25 |
Required | Expected | Actual | Pending | Missing | Requested |
2a: Yield Requested | +105 | +105 | 0 | +105 | 0 |
2b: Partial Yield Received | +80 | –80 | - |
2c: Yield Timeout | -25 | –25 | +25 |
2d: Lab Requeue for Additional Yield | +30 | +30 | –25 | +30 |
Total |
Required | Expected | Actual | Pending | Missing | Requested |
2a: Yield Requested | +105 | +105 | 0 | +105 | 0 |
2b: Partial Yield Received | +80 | –80 | - |
2c: Yield Timeout | -25 | –25 | +25 |
2d: Lab Requeue for Additional Yield | +30 | +30 | –25 | +30 |
2e: Balance of Yield Received | +17 | +47 | –30 |
Total | 105 | 127 | 127 | 0 | 0 | 30 |
Required | Expected | Actual | Pending |
2a: Yield Requested | +105 | +105 | 0 | +105 |
Total | 105 | 105 | 0 | 105 |
Required | Expected | Actual | Pending |
2a: Yield Requested | +105 | +105 | 0 | +105 |
2b: Partial Yield Received | +80 | –80 |
Total | 105 | 105 | 80 | 25 |