# Request A Lab Requeue

Biosamples that have not produced the requested amount of yield are marked as Missing Yield. Use a Lab Requeue to request more sequencing yield from the lab. After the requeue request is set to Acknowledged, incoming yield is applied to the request. For more information about yield calculations, see [Yield](https://help.connected.illumina.com/basespace/automate/yield).

A lab requeue requests additional biosample data. For information about requeueing a run with a fixed sample sheet, see [Fix Sample Sheet](https://help.connected.illumina.com/basespace/runs/fix-sample-sheet).

The lab requeue returns the biosample to one of three stages in the lab preparation process.

* **Biosample** — The original biosample is prepared in a new library for sequencing.
* **Library** — An existing library is used for sequencing.
* **Pool** — An existing pool is used for sequencing. You can track the status or cancel the requeue from the Requeues tab of the biosample details page.

### Requeue a Biosample

Request a Biosample Requeue if there were problems with the sequencing data from a biosample, or the existing library or pool does not have enough volume to produce the required yield. This request initiates a new library preparation from the original biosample.

1. Open the biosample details page for the biosample you want to requeue.
2. Select the **Summary** tab.
3. Select the **File** drop-down arrow, point to **Requeue**, and then select **Biosample**.
4. In the Requeue Biosample dialog, specify the requeue parameters.
   * Library Prep kit
   * Requested yield (Gbp/Mbp)
5. Select **Save**.

### Requeue a Library

1. Open the biosample details page for the biosample you want to requeue.
2. Select the **Libraries** tab.
3. Select the checkbox for the library you want to requeue. You can only requeue one library at a time.
4. Select the **File** drop-down arrow, point to **Lab Requeue**, and then select **Library**.
5. In the Requeue Library dialog, specify the requeue parameters.
   * Requested yield
6. Select **Save**.

### Requeue a Pool

1. Open the biosample details page for the biosample you want to requeue.
2. Select the **Libraries** tab.
3. Select the pool name for the desired library. If more than one pool exists for the library, select **Pools**, and then select the desired pool from the list.
4. Select the **File** drop-down arrow, point to **Lab Requeue**, and then select **Pool**.
5. In the Requeue Pool dialog, specify the requeue parameters.
   * Required pool yield
   * Unit of measurement
6. Select **Save**.

### Acknowledge a Lab Requeue Request

Pending Lab Requeues are listed the Requeues tab of the Biosample Details page. When you have determined that your lab can fulfill the request, change the status to Acknowledged.

For information about acknowledging requeues using the BaseSpace Sequence Hub API, see the developer documentation at [developer.basespace.illumina.com](https://developer.basespace.illumina.com/).

{% hint style="info" %}
If the requeue is not acknowledged, incoming yield is applied to the original prep request.
{% endhint %}

### Cancel a Requeue

You can cancel a pending requeue. You might do this if the requeue is not needed, or there were errors in the requeue.

1. Open the biosample details page for the requeued biosample.
2. Select the **Requeues** tab.
3. To cancel a library, do the following:
   1. Select a library.
   2. Select the **Status** drop-down arrow, point to **Cancel**, and then select **Library Requeue**
4. To cancel a pool, do the following:
   1. Select a pool.
   2. Select the **Status** drop-down arrow, point to **Cancel**, and then select **Pool Requeue**