Release Notes

Last Updated: November 2024

Release Date: June 2024

Document Version: 2

These release notes describe the key changes to Illumina Preset Protocols (IPP) v2.8.

Compatibility

Refer to Compatibility under Instruments & Integrations.

New Features

• Added a new NextSeq 1000/2000 On-Prem Sequencing v1.0 protocol that supports the NextSeq 1000/2000 On-Prem Integration Package v1.0.

• Updated the NextSeq 1000/2000 Sequencing v2.4 protocol to support NextSeq 1000/2000 Integration Package v2.5.

• Updated the following protocols to support the new Illumina DNA/RNA UD Indexes Sets A–D:

  • Illumina COVIDSeq Workflow

  • Illumina DNA PCR-Free Library Prep Manual

  • Illumina DNA Prep (M) Tagmentation

  • Illumina Stranded mRNA Prep Ligation

  • Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus

  • Illumina DNA Prep with Enrichment (S) Tagmentation

  • Illumina RNA Prep with Enrichment (L) Tagmentation

• Updated the sequencing instrument presets and routing scripts to remove the HiSeq series of instruments.

• Updated the step output naming convention for the Library Prep Validation protocol.

Defects Fixed

• Removed Sample_Well field from MiSeq-generated sample sheet for sample sheet to be compatible with the MiSeq RUO Local Run Manager v4.1.

Known Issues

• There is an import issue when you try to import TruSeq RNA Exome v1.05 from IPP v2.8 after it has been imported to Clarity LIMS from IPP v2.7. This issue is due to a missed protocol version upgrade for the TruSeq RNA Exome in IPP v2.8.

• Due to a known with the config slicer tool, importing workflows and protocols from IPP on Clarity v6.2 and v6.3 requires longer time (~ 8 times) compared to earlier versions (Clarity v6.1 and below).

Revision History

Version	Changes
2	Updated Compatibility section to reference Compatibility matrix table.
1	Initial release.