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NextSeq 500/550 v2.3.0

The Illumina NextSeq 500/550 Integration v2.3.0 includes the following features and functionality:

  • Added support for Oracle Linux. For compatibility, refer to NextSeq 500/550 Integration v2.3.0 Release Notes.

  • A new NextSeq 500/550 v1.1 workflow that maps to lab protocols and instrument runs. This workflow includes a minor bug fix and has no additional changes from the previous version.

  • Automated generation of the sample sheet. This sample sheet is used with the bcl2fastq2 v2.20.0 analysis software.

  • Automated tracking of the NextSeq sequencing runs and parsing of run statistics into Clarity LIMS, which includes the following information:

    • Sequencing run metrics

    • Sequencing run parameters

    • Real-Time Analysis v2 (RTA2) run directory location and other run-specific information

  • Preconfigured Library Prep Validation v2.3.1 workflow used for validation purposes. This workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing v1.1 workflow. For more information, refer to NextSeq 500/550 Integration v2.3.0 User Interaction, Validation and Troubleshooting.

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