RNA Initial QC 5.1.2

Overview

The initial RNA QC protocol contains steps to track quality control data from commonly used QC instruments. Through data integrations, QC data can be added to Clarity LIMS directly from the instrument outputs.

Highlights of this protocol include:

• Data integrations for Bioanalyzer 2100, Nanodrop 8000, CaliperGX, and Tapestation 2200, including creation of the input file and parsing of data output.

• Assignment of pass/fail QC based on chosen criteria.

• Aggregation of data from multiple QC steps.

Protocol 1: RNA Initial QC 5.1.2

• Protocol Type = QC

• Capacity = 50

• QC Filters

  • Bioanalyzer QC (RNA) 5.1.2

    • Did not pass - Bioanalyzer QC (RNA) 5.1.2

  • NanoDrop QC (RNA) 5.1.2

    • Did not pass - NanoDrop QC (RNA) 5.1.2

  • Qubit QC (RNA) 5.1.2

    • Did not pass - Qubit QC (RNA) 5.1.2

  • PicoGreen QC (RNA) 5.1.2

    • Did not pass - PicoGreen QC (RNA) 5.1.2

  • CaliperGX QC 5.1.2

    • Did not pass - CaliperGX QC 5.1.2

  • Tapestation QC (RNA) 5.1.2

    • Did not pass - Tapestation QC (RNA) 5.1.2

  • Aggregate QC (RNA) 5.1.2

    • Passed - Bioanalyzer QC (RNA) 5.1.2 OR

    • Passed - NanoDrop QC (RNA) 5.1.2 OR

    • Passed - Qubit QC (RNA) 5.1.2 OR

    • Passed - PicoGreen QC (RNA) 5.1.2 OR

    • Passed - CaliperGX QC 5.1.2 OR

    • Passed - Tapestation QC (RNA) 5.1.2

Step: Bioanalyzer QC (RNA) 5.1.2

• Master Step Name = Bioanalyzer QC (RNA) 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} Bioanalyzer

Automations

Generate Bioanalyzer driver file

• Trigger Location = Record Details

• Trigger Style = Automatic upon entry

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
      script:driver_file_generator \
        -i {processURI:v2} \
        -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv \
        -o {compoundOutputFileLuid0}.csv \
        -l {compoundOutputFileLuid1} \
      && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
      script:addBlankLines \
        -i {stepURI:v2} \
        -f {compoundOutputFileLuid0}.csv \
        -l {compoundOutputFileLuid1} \
        -sep COMMA \
        -b ',False,' \
        -h 1 \
        -c LIMSID \
        -pre 'Sample '"

Parse Bioanalyzer XML and assign QC flags

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {processURI:v2} \
      script:parseBioAnalyzer \
        -inputFile {compoundOutputFileLuid2} \
        -log {compoundOutputFileLuid5} \
        -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerRNAConfig.groovy' \
      script:assignQC \
        -log {compoundOutputFileLuid6} \
        -qcResult {compoundOutputFileLuid7}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in
    Submitted Sample	Sample Type	Text

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Derived Sample	Workflow	Text
    Project	Project Name	Built-in

Placement = Enabled

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

  • Placement Pattern = Column

• Destination Containers

  • BioAnalyzer RNA Nano Chip

  

  * BioAnalyzer RNA Pico Chip

  

Record Details

• Step Data

  • Step Data Heading = Begin by uploading the required Bioanalyzer XML result file

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Criteria 1 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 1 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units RIN RNA Area 28s/18s ratio Number of Peaks found Peak 1 Size - bp Peak 1 Conc. Peak 1 Molarity Peak 2 Size - bp Peak 2 Conc. Peak 2 Molarity Peak 3 Size - bp Peak 3 Conc. Peak 3 Molarity Peak 4 Size - bp Peak 4 Conc. Peak 4 Molarity Peak 5 Size - bp Peak 5 Conc. Peak 5 Molarity Number of Regions found Region 1 Average Size - bp Region 1 Conc. Region 1 Molarity Region 2 Average Size - bp Region 2 Conc. Region 2 Molarity Region 3 Average Size - bp Region 3 Conc. Region 3 Molarity Region 4 Average Size - bp Region 4 Conc. Region 4 Molarity Region 5 Average Size - bp Region 5 Conc. Region 5 Molarity
    Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 2
    Criteria 2 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 2 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units RIN RNA Area 28s/18s ratio Number of Peaks found Peak 1 Size - bp Peak 1 Conc. Peak 1 Molarity Peak 2 Size - bp Peak 2 Conc. Peak 2 Molarity Peak 3 Size - bp Peak 3 Conc. Peak 3 Molarity Peak 4 Size - bp Peak 4 Conc. Peak 4 Molarity Peak 5 Size - bp Peak 5 Conc. Peak 5 Molarity Number of Regions found Region 1 Average Size - bp Region 1 Conc. Region 1 Molarity Region 2 Average Size - bp Region 2 Conc. Region 2 Molarity Region 3 Average Size - bp Region 3 Conc. Region 3 Molarity Region 4 Average Size - bp Region 4 Conc. Region 4 Molarity Region 5 Average Size - bp Region 5 Conc. Region 5 Molarity
    Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 2
    Use strict matching for Bioanalyzer results	Toggle Switch		Default = None Set

• Step File Placeholders

  • Bioanalyzer Driver File - Automatically attached

  • Bioanalyzer Driver File Generation Log File - Automatically attached

  • Bioanalyzer XML Result File (required) - Manually uploaded

  • Result File (optional) - Manually uploaded

  • PDF Summary File (optional) - Manually uploaded

  • Bioanalyzer XML Parsing Log File - Automatically attached

  • QC Assignment Log File - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Measurement	28s/18s ratio	Numeric		Decimal Places Displayed = 2
    Measurement	BA Sample Name	Text
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Conc. Units	Text
    Measurement	Number of Peaks found	Numeric		Decimal Places Displayed = 0
    Measurement	Number of Regions found	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 1 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 1 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 1 Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 2 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 2 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 2 Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 3 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 3 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 3 Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 4 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 4 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 4 Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 5 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 5 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 5 Size - bp	Numeric		Decimal Places Displayed = 2
    Measurement	Region 1 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 1 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 1 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 2 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 2 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 2 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 3 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 3 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 3 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 4 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 4 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 4 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 5 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 5 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 5 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	RIN	Numeric		Decimal Places Displayed = 2
    Measurement	RNA Area	Numeric		Decimal Places Displayed = 2
    Measurement	Sample Comment	Text
    Project	Project Name	Built-in

Step: NanoDrop QC (RNA) 5.1.2

• Master Step Name = NanoDrop QC (RNA) 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} NanoDrop

Automations

Generate NanoDrop driver file

• Trigger Location = Record Details

• Trigger Style = Automatic upon entry

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
      script:driver_file_generator \
        -i {processURI:v2} \
        -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/nd_driver_file_template.csv \
        -o {compoundOutputFileLuid0}.csv \
        -l {compoundOutputFileLuid1}"

Parse NanoDrop NDT and assign QC flags

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {processURI:v2} \
      script:parseCSV \
        -inputFile {compoundOutputFileLuid2} \
        -log {compoundOutputFileLuid3} \
        -headerRow '2' \
        -separator 'TAB' \
        -sampleLocation 'Sample ID' \
        -measurementUDFMap 'Concentration::ng/ul' \
        -measurementUDFMap 'A260/280 ratio::260/280' \
        -measurementUDFMap 'A260/230 ratio::260/230' \
        -measurementUDFMap 'A260::260' \
        -relaxed 'true' \
      script:assignQC \
        -log {compoundOutputFileLuid4} \
        -qcResult {compoundOutputFileLuid5}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in
    Submitted Sample	Sample Type	Text

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Derived Sample	Workflow	Text
    Project	Project Name	Built-in

Record Details

• Step Data

  • Step Data Heading = Begin by uploading the required NanoDrop NDT result file

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Criteria 1 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 1 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units A260 A260/280 ratio A260/230 ratio
    Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 2
    Criteria 2 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 2 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units A260 A260/280 ratio A260/230 ratio
    Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 2

• Step File Placeholders

  • NanoDrop Driver File - Automatically attached

  • NanoDrop Driver File Generation Log File - Automatically attached

  • NanoDrop NDT Result File (required) - Manually uploaded

  • NanoDrop NDT Parsing Log File - Automatically attached

  • QC Assignment Log File - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Measurement	A260	Numeric		Decimal Places Displayed = 2
    Measurement	A260/230 ratio	Numeric		Decimal Places Displayed = 2
    Measurement	A260/280 ratio	Numeric		Decimal Places Displayed = 2
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Conc. Units	Text
    Project	Project Name	Built-in

Step: Qubit QC (RNA) 5.1.2

• Master Step Name = Qubit QC (RNA) 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} Qubit

Automations

Assign QC flags

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
      script:assignQC \
        -i {processURI:v2} \
        -log {compoundOutputFileLuid1} \
        -qcResult {compoundOutputFileLuid2}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in
    Submitted Sample	Sample Type	Text

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Derived Sample	Workflow	Text
    Project	Project Name	Built-in

Record Details

• Step Data

  • Step Data Heading = Begin by completing the Sample Details table

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Criteria 1 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 1 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units
    Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 2
    Criteria 2 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 2 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units
    Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 2

• Step File Placeholders

  • Qubit Result File - Manually uploaded

  • QC Assignment Log File - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Conc. Units	Text
    Project	Project Name	Built-in

Step: PicoGreen QC (RNA) 5.1.2

• Master Step Name = PicoGreen QC (RNA) 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} PicoGreen

Automations

Assign QC flags

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
      script:assignQC \
        -i {processURI:v2} \
        -log {compoundOutputFileLuid1} \
        -qcResult {compoundOutputFileLuid2}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Containers

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in
    Submitted Sample	Sample Type	Text

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Derived Sample	Workflow	Text
    Project	Project Name	Built-in

Placement = Enabled

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

  • Placement Pattern = Column

• Destination Containers

  • Tube

  • 96 well plate

Record Details

• Step Data

  • Step Data Heading = Begin by completing the Sample Details table

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Criteria 1 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 1 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units
    Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 2
    Criteria 2 - Operator	Text Dropdown		Presets >= <= = !=
    Criteria 2 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units
    Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 2

• Step File Placeholders

  • PicoGreen Result File - Manually uploaded

  • QC Assignment Log File - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Concentration	Numeric	Required Field	Decimal Places Displayed = 2
    Derived Sample	Conc. Units	Text	Required Field
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Conc. Units	Text
    Project	Project Name	Built-in

Step: CaliperGX QC 5.1.2

• Master Step Name = CaliperGX QC 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} CaliperGX

Automations

Generate Driver File

• Trigger Location = Record Details

• Trigger Style = Automatic upon entry

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
      script:driver_file_generator \
        -i {processURI:v2} \
        -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/caliperGX_driver_file_template.csv \
        -o {compoundOutputFileLuid0}.csv \
        -l {compoundOutputFileLuid1}"

Parse CaliperGX CSV

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {processURI:v2} \
      script:parseCSV \
        -inputFile {compoundOutputFileLuid2} \
        -log {compoundOutputFileLuid3} \
        -headerRow '1' \
        -separator 'comma' \
        -containerName 'Plate Name' \
        -wellPosition 'Well Label' \
        -measurementUDFMap 'Concentration::Region[100-1000] Conc. (ng/ul)' \
        -measurementUDFMap 'Size (bp)::Region[100-1000] Size at Maximum [BP]' \
        -relaxed 'true' \
        -matchOutput 'true' \
      script:assignQC \
        -log {compoundOutputFileLuid4} \
        -qcResult {compoundOutputFileLuid5}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	LIMS ID (Container)	Built-in
    Project	Project Name	Built-in

Placement = Enabled

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

  • Placement Pattern = Column

• Destination Containers

  • 384 well plate

  • 96 well plate

Record Details

Group of Defaults

Caliper Defaults

• Criteria 1 - Operator = >=

• Criteria 1 - Source Data Field = Concentration

• Criteria 1 - Threshold Value = 0

• Criteria 2 - Operator = >=

• Criteria 2 - Source Data Field = Size (bp)

• Criteria 2 - Threshold Value = 300

• Step Data

  • Group of Defaults = Caliper Defaults

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Criteria 1 - Operator	Text Dropdown	Custom Entries	Presets >= <= = !=
    Criteria 1 - Source Data Field	Text Dropdown	Custom Entries	Presets Concentration
    Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 0
    Criteria 2 - Operator	Text Dropdown	Custom Entries	Presets >= <= = !=
    Criteria 2 - Source Data Field	Text Dropdown	Custom Entries	Presets Size (bp)
    Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 0

• Step File Placeholders

  • Driver File - Automatically attached

  • Driver File Log - Automatically attached

  • CaliperGX WellTable (required) - Manually uploaded

  • CaliperGX Parsing Log File - Automatically attached

  • QC Assignment Log File - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	LIMS ID (Container)	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Size (bp)	Numeric		Decimal Places Displayed = 0
    Project	Project Name	Built-in

Step: Tapestation QC (RNA) 5.1.2

• Master Step Name = Tapestation QC (RNA) 5.1.2

• Step Type = Standard QC

• Measurement Generation = Fixed, 1

• Naming Convention = {InputItemName} Tapestation

Automations

Generate Tapestation Input Sampletable CSV

• Trigger Location = Record Details

• Trigger Style = Automatic upon entry

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
      script:driver_file_generator \
        -i {processURI:v2} \
        -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/tapestation_driver_file_template.csv \
        -o {compoundOutputFileLuid0}.csv \
        -l {compoundOutputFileLuid1}"

Parse Tapestation XML and assign QC flags

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/XmlSampleNameParser.jar -u {username} -p {password} \
      script:parseXmlBySampleName \
        -i {processURI:v2} \
        -inputFile {compoundOutputFileLuid2} \
        -log {compoundOutputFileLuid3}.html \
        -configFile /opt/gls/clarity/extensions/conf/v5/tapestation/defaultTapeStationRNAConfig.groovy \
      && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
      script:assignQC \
        -i {processURI:v2} \
        -log {compoundOutputFileLuid4} \
        -qcResult {compoundOutputFileLuid5}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	LIMS ID (Container)	Built-in
    Project	Project Name	Built-in

Record Details

• Step Data (Master Step Fields)

  Field Name	Field Type	Options	Additional Options and Dropdown Items
  Criteria 1 - Operator	Text Dropdown		Presets >= <= = != Default = >=
  Criteria 1 - Source Data Field	Text Dropdown		Presets Conc. Units Concentration Molarity Units MW Units Peak 1 % Integrated Area Peak 1 Conc. Peak 1 MW Peak 2 % Integrated Area Peak 2 Conc. Peak 2 MW Peak 3 % Integrated Area Peak 3 Conc. Peak 3 MW Peak 4 % Integrated Area Peak 4 Conc. Peak 4 MW Peak 5 % Integrated Area Peak 5 Conc. Peak 5 MW Region 1 % of Total Region 1 Average Size - bp Region 1 Conc. Region 1 Molarity Region 2 % of Total Region 2 Average Size - bp Region 2 Conc. Region 2 Molarity Region 3 % of Total Region 3 Average Size - bp Region 3 Conc. Region 3 Molarity Region 4 % of Total Region 4 Average Size - bp Region 4 Conc. Region 4 Molarity Region 5 % of Total Region 5 Average Size - bp Region 5 Conc. Region 5 Molarity Default = Conc. Units
  Criteria 1 - Threshold Value	Numeric		Decimal Places Displayed = 0
  Criteria 2 - Operator	Text Dropdown		Presets >= <= = != Default = >=
  Criteria 2 - Source Data Field	Text Dropdown		Presets Concentration Conc. Units Peak 1 Conc. Peak 2 Conc. Peak 3 Conc. Peak 4 Conc. Peak 5 Conc. Region 1 Average Size - bp Region 1 Conc. Region 1 Molarity Region 2 Average Size - bp Region 2 Conc. Region 2 Molarity Region 3 Average Size - bp Region 3 Conc. Region 3 Molarity Region 4 Average Size - bp Region 4 Conc. Region 4 Molarity Region 5 Average Size - bp Region 5 Conc. Region 5 Molarity Region 1 % of Total Region 2 % of Total Region 3 % of Total Region 4 % of Total Region 5 % of Total Peak 1 MW Peak 2 MW Peak 3 MW Peak 4 MW Peak 5 MW Molarity Units Peak 1 % Integrated Area Peak 2 % Integrated Area Peak 3 % Integrated Area Peak 4 % Integrated Area Peak 5 % Integrated Area MW Units Default = Concentration
  Criteria 2 - Threshold Value	Numeric		Decimal Places Displayed = 0

• Step File Placeholders

  • TapeStation Input SampleTable - Automatically attached

  • Sample Table Generation Log - Automatically attached

  • Tapestation Output XML (required) - Manually uploaded

  • Tapestation XML Parsing Log - Automatically attached

  • QC Assignment Log - Automatically attached

  • QC Assignment Report - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • File Column Options

    • File Column Display = Hide

    • File Attachment Method = Auto

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	LIMS ID (Container)	Built-in
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Measurement	28s/18s ratio	Numeric		Decimal Places Displayed = 2
    Measurement	Concentration	Numeric		Decimal Places Displayed = 2
    Measurement	Conc. Units	Text
    Measurement	Molarity Units	Text
    Measurement	MW Units	Text
    Measurement	Peak 1 % Integrated Area	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 1 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 1 MW	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 2 % Integrated Area	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 2 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 2 MW	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 3 % Integrated Area	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 3 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 3 MW	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 4 % Integrated Area	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 4 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 4 MW	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 5 % Integrated Area	Numeric		Decimal Places Displayed = 2
    Measurement	Peak 5 MW	Numeric		Decimal Places Displayed = 0
    Measurement	Peak 5 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 1 % of Total	Numeric		Decimal Places Displayed = 2
    Measurement	Region 1 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 1 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 1 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 2 % of Total	Numeric		Decimal Places Displayed = 2
    Measurement	Region 2 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 2 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 2 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 3 % of Total	Numeric		Decimal Places Displayed = 2
    Measurement	Region 3 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 3 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 3 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 4 % of Total	Numeric		Decimal Places Displayed = 2
    Measurement	Region 4 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 4 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 4 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	Region 5 % of Total	Numeric		Decimal Places Displayed = 2
    Measurement	Region 5 Average Size - bp	Numeric		Decimal Places Displayed = 0
    Measurement	Region 5 Conc.	Numeric		Decimal Places Displayed = 2
    Measurement	Region 5 Molarity	Numeric		Decimal Places Displayed = 2
    Measurement	RIN	Numeric		Decimal Places Displayed = 2
    Project	Project Name	Built-in

Step: Aggregate QC (RNA) 5.1.2

• Master Step Name = Aggregate QC (RNA) 5.1.2

• Step Type = No Outputs

Automations

Aggregate QC Flags and Copy Fields

• Trigger Location = Record Details

• Trigger Style = Manual button

bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
      script:setUDF \
        -i {processURI:v2} \
        -f 'Progress' \
        -t '//input/@uri->//sample/@uri' \
        -v 'Initial sample QC complete' \
      script:aggregateQC \
        -i {stepURI:v2} \
        -c 'true' \
        -a 'true' \
        -log {compoundOutputFileLuid0} \
        -aggregatelog {compoundOutputFileLuid1} \
        -copylog {compoundOutputFileLuid2}"

Queue/Ice Bucket

• Defaults

  • Sample Grouping = Group by Projects

  • Well Sort Order = Row

• Sample Table

  • Column Headers

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Well	Built-in
    Derived Sample	Sample Name	Built-in
    Derived Sample	Waiting	Built-in
    Derived Sample	Workflow	Text
    Project	Project Name	Built-in

  • Expanded View Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in

Record Details

Group of Defaults

CaliperGX Values

• Bioanalzyer QC (RNA) 5.1.2 = Use if available (Priority 5)

• CaliperGX QC 5.1.2 = Use if available (Priority 5)

• Copy task 1 - Source Field = Concentration

• Copy task 1 - Source Step = CaliperGX QC 5.1.2

• Copy task 2 - Source Field = Conc. Units

• Copy task 2 - Source Step = CaliperGX QC 5.1.2

• NanoDrop QC (RNA) 5.1.2 = Use if available (Priority 5)

• PicoGreen QC (RNA) 5.1.2 = Use if available (Priority 5)

• Qubit QC (RNA) 5.1.2 = Use if available (Priority 5)

• Tapestation QC (RNA) 5.1.2 = Use if available (Priority 5)

• Step Data

  • Step Data Heading = Begin by verifying copy tasks and aggregation priorities

  • Group of Defaults = CaliperGX Values

  • Master Step Fields

    Field Name	Field Type	Options	Additional Options and Dropdown Items
    Copy task 1 - Source Field	Text Dropdown		Presets Concentration Conc. Units
    Copy task 1 - Source Step	Text Dropdown		Presets Bioanalyzer QC (RNA) 5.1.2 NanoDrop QC (RNA) 5.1.2 PicoGreen QC (RNA) 5.1.2 Qubit QC (RNA) 5.1.2 CaliperGX QC 5.1.2 Tapestation QC (RNA) 5.1.2
    Copy task 2 - Source Field	Text Dropdown		Presets Concentration Conc. Units
    Copy task 2 - Source Step	Text Dropdown		Presets Bioanalyzer QC (RNA) 5.1.2 NanoDrop QC (RNA) 5.1.2 PicoGreen QC (RNA) 5.1.2 Qubit QC (RNA) 5.1.2 CaliperGX QC 5.1.2 Tapestation QC (RNA) 5.1.2
    Copy task 3 - Source Field	Text Dropdown		Presets Concentration Conc. Units A260/280 ratio
    Copy task 3 - Source Step	Text Dropdown	Custom Entries	Presets Bioanalyzer QC (RNA) 5.1.2 NanoDrop QC (RNA) 5.1.2 PicoGreen QC (RNA) 5.1.2 Qubit QC (RNA) 5.1.2 CaliperGX QC 5.1.2 Tapestation QC (RNA) 5.1.2

• Step File Placeholders

  • Aggregation Script Log - Automatically attached

  • QC Flag Aggregation Log - Automatically attached

  • QC UDF Copy Log - Automatically attached

• Sample Table

  • Enable QC Flags = Yes

  • Sample Display Default = Collapse

  • Well Sort Order = Row

  • Table Columns - Global Fields

    Category	Field Name	Field Type	Options	Additional Options and Dropdown Items
    Container	Container Name	Built-in
    Container	Well	Built-in
    Derived Sample	Concentration	Numeric	Required Field	Decimal Places Displayed = 2
    Derived Sample	Conc. Units	Text	Required Field
    Derived Sample	Sample Name	Built-in
    Project	Project Name	Built-in