Last Updated: November 2024

Release Date: July 2024

Document Version: 2

These release notes describe the key changes to software components for the Clarity LIMS NextSeq 1000/2000 Integration Package v2.5.1.

Compatibility

Refer to Compatibility under Instruments & Integrations.

Defect Repairs

• Fixed the issue of unable to generate sample sheet and planned run when any sample in the pool has been assigned QC flag prior to entering the Load to Reagent Cartridge step.

Known Issues

• Sequencing run error triggered by disk space full on instrument causes RunParameters.xml to be empty and the run event cannot be processed on Clarity LIMS. This error is unlikely to occur as pre-run checks on the instrument check for sufficient disk space before run is started.

Documentations

The NextSeq 1000/2000 Integration v2.5.1 guides follow exactly that of the following NextSeq 1000/2000 Integration v2.5.0 with the following modifications:

• NextSeq 1000/2000 Integration v2.5.0 Configuration

  • NextSeq 1000/2000 Integration Package version has been updated from v2.5.0 to v2.5.1.

• NextSeq 1000/2000 Integration v2.5.0 User Interaction, Validation and Troubleshooting

  • NextSeq 1000/2000 Integration Package version has been updated from v2.5.0 to v2.5.1.

Revision History

The integration includes the following features:

• Preconfigured NextSeq 1000/2000 Sequencing v2.4 workflow that maps to lab protocols and instrument runs.

• The following preconfigured protocols:

  • Library Pooling and Dilution (NextSeq 1000/2000 Sequencing v2.4)

  • Load To Reagent Cartridge (NextSeq 1000/2000 Sequencing v2.4)

  • AUTOMATED - Sequencing Run (NextSeq 1000/2000 Sequencing v2.4)

  • Demultiplexing (NextSeq 1000/2000 Sequencing v2.4)

• Automatic validation of run setup information before sample sheet generation or planned run creation.

• Automated generation of a sample sheet. The sample sheet is used to start the sequencing run on NextSeq 1000/2000 Control Software (NCS) via Local run mode.

• Automated creation of a planned run. The NCS retrieves and starts the planned run via Hybrid or Cloud run mode.

• Automated tracking of the NextSeq 1000/2000 sequencing run and parsing of run statistics from BaseSpace Sequence Hub into Clarity LIMS.

• Automated tracking of the secondary DRAGEN analysis (up to BCL Convert only) and parsing of demultiplexing metrics from BaseSpace Sequence Hub into Clarity LIMS.

• The following Cloud analysis supported applications:

  • BCL Convert v4.2.7 or earlier under BaseSpace Sequence Hub DRAGEN Analysis

  • BCL Convert v4.2.7 under ICA Workflow Session Tracking

• Local analysis supported BCL Convert v4.2.7 and earlier.

• [Optional] Preconfigured Library Prep Validation workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep workflow required to produce libraries tagged with index sequences. At the end of the step, these libraries are routed to NextSeq 1000/2000 Sequencing v2.4 workflow.

• [Optional] An integration of Clarity LIMS Product Analytics (CLPA) that make Clarity LIMS data available to the ICA and CLPA. This integration also requires installation and configuration. For more information, refer to .