TruSight Oncology 500 v1.1
Protocol 1: DNA/RNA FFPE Extraction (TruSight Oncology 500 v1.1)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: DNA/RNA FFPE Extraction (TruSight Oncology 500 v1.1)
Master Step Name = DNA/RNA FFPE Extraction (TSO 500 HT v1.1)
Derived Sample Generation = Fixed, 2
Set Sample Type
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Remove
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Route DNA/RNA samples
Trigger Style = Automatic upon exit
Route DNA/RNA samples - HT
Trigger Location = Not Used
ℹ The actual version of the workflows and steps in the routing automation script may be different depending on the version of IPP installed.
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Protocol 2: RNA QC and cDNA Synthesis (TruSight Oncology 500 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Qubit RNA QC (TruSight Oncology 500 v1.1)
Master Step Name = Qubit RNA QC (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Assign QC flags - RNA
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Denature and Anneal RNA (TruSight Oncology 500 v1.1)
Master Step Name = Denature and Anneal RNA (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
RNA Sample Dilution
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 3: First Strand cDNA Synthesis (TruSight Oncology 500 v1.1)
Master Step Name = First Strand cDNA Synthesis v2.0
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 4: Second Strand cDNA Synthesis (TruSight Oncology 500 v1.1)
Master Step Name = Second Strand cDNA Synthesis (TruSight Oncology 500 HT v1.1)
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 5: cDNA Clean Up (TruSight Oncology 500 v1.1)
Master Step Name = cDNA Clean Up (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Assign Sample Type
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Remove
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Routing cDNA Samples
Trigger Style = Automatic upon exit
Routing cDNA Samples - HT
Trigger Location = Not Used
ℹ The actual version of the workflows and steps in the routing automation script may be different depending on the version of IPP installed.
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Protocol 3: DNA QC and Fragmentation (TruSight Oncology 500 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Qubit DNA QC (TruSight Oncology 500 v1.1)
Master Step Name = Qubit DNA QC (TruSight Oncology 500 HT v1.1)
Measurement Generation = Fixed, 1
Assign QC flags - DNA
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Fragment DNA (TruSight Oncology 500 v1.1)
Master Step Name = Fragment DNA (TruSight Oncology 500 HT v1.1)
Derived Sample Generation = Fixed, 1
Dilution Calculation
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Protocol 4: Library Prep (TruSight Oncology 500 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: End Repair and A-Tailing (TruSight Oncology 500 v1.1)
Master Step Name = End Repair and A-Tailing (TruSight Oncology 500 HT v1.1)
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Ligate Adapters (TruSight Oncology 500 v1.1)
Master Step Name = Ligate Adapters (TruSight Oncology 500 HT v1.1)
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 3: Clean Up Ligation (TruSight Oncology 500 v1.1)
Master Step Name = Clean Up v2.0
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 4: Index PCR (TruSight Oncology 500 v1.1)
Master Step Name = Index PCR (TruSight Oncology 500 HT v1.1)
Derived Sample Generation = Fixed, 1
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Label Groups
TruSight Oncology 500 NextSeq
Step Data (Master Step Fields)
Protocol 5: Hybridization (TruSight Oncology 500 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Set Up First Hybridization (TruSight Oncology 500 v1.1)
Master Step Name = Hybridize v2.0
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Library Dilution
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Capture Targets One (TruSight Oncology 500 v1.1)
Master Step Name = Capture Targets One (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 3: Set Up Second Hybridization (TruSight Oncology 500 v1.1)
Master Step Name = Set Up Second Hybridization (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 4: Capture Targets Two (TruSight Oncology 500 v1.1)
Master Step Name = Capture Targets Two (TSO 500 v1.1)
Derived Sample Generation = Fixed, 1
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 5: Amplify Enriched Library (TruSight Oncology 500 v1.1)
Master Step Name = Amplify Enriched Library v2.0
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 6: Clean Up Amplified Enriched Library (TruSight Oncology 500 v1.1)
Master Step Name = Clean Up v2.0
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
