iLASS Infinium Genotyping with PGx Assay v1.0
The following global fields are used by the workflow. If these fields are already defined, they do not need to be added again.
From Configuration, select the Custom Fields tab.
Under Global Fields, select Container.
Under Field Type, select Text.
Under Field Options, make the following selections:
Under Field Type, select Multiline Text.
Under Field Options, make the following selections:
Protocol 1: Automated Target Prep with PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Select First Workflow Step (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Select First Workflow Step with PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Select First Step with PGx v1.0
Trigger Location = Record Details
Trigger Style = Manual button
Step Data (Master Step Fields)
Step 2: Batch DNA Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name: Batch DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Derived Sample Generation = Fixed, 1
Placement = Enabled
Sample Grouping = Group by Containers
Placement Pattern = Same Shape
Step Data (Master Step Fields)
Step 3: Make WGA and PGx Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Make WGA and PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Derived Sample Generation = Fixed, 2
Placement = Enabled
Sample Grouping = Group by Containers
Placement Pattern = Same Shape
Step Data (Master Step Fields)
Step 4: Make WGA and PGx Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Make WGA and PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Derived Sample Generation = Fixed, 2
Placement = Enabled
Sample Grouping = Group by Containers
Placement Pattern = Same Shape
Step Data (Master Step Fields)
Step 5: Amplify WGA Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Amplify WGA Manual (ILASS Infinium Genotyping Assay v1.0)
Set Next Step - Advance v1.0
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Step Data (Master Step Fields)
Step 6: Amplify PGx Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Amplify PGx Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Set Next Step - Advance v1.0
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Step Data (Master Step Fields)
Step 7: Recombine and Fragment DNA Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Recombine and Fragment DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Aliquot Generation = Fixed, 1
Sample Grouping = Group by Containers
Placement = Enabled
Step Data (Master Step Fields)
Step 8: Precipitate DNA Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Precipitate DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Data (Master Step Fields)
Step 9: Resuspend DNA Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Resuspend DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Data (Master Step Fields)
Protocol 2: Automated BeadChip Processing with PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Hybridize DNA to the BeadChip Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Hybridize DNA to the BeadChip Automated (ILASS Infinium Genotyping Assay v1.0)
Derived Sample Generation = Fixed, 1
Placement = Enabled
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Prepare Hyb Chamber Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Prepare Hyb Chamber Manual (ILASS Infinium Genotyping Assay v1.0)
Set Next Step - Advance v1.0
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Step Data (Master Step Fields)
Step 3: Wash BeadChips Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Wash BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Set Next Step - Advance v1.0
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Step Data (Master Step Fields)
Step 4: Extend and Stain BeadChips Automated (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Extend and Stain BeadChips Automated (ILASS Infinium Genotyping Assay v1.0)
Step Data (Master Step Fields)
Step 5: Wash and Coat BeadChips Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Wash and Coat BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Step Data (Master Step Fields)
Protocol 3: Infinium Array Imaging on iScan (ILASS Infinium Genotyping Assay with PGx v1.0)
Protocol Type = Other
Next Steps Configuration
Step 1: Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay v1.0)
Set Next Step - Advance v1.0
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Step Data (Master Step Fields)
Protocol 4: Infinium Analysis (ILASS Infinium Genotyping Assay with PGx v1.0)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Invoke Infinium Analysis (ILASS Infinium Genotyping Assay with PGx v1.0)
Master Step Name = Invoke Infinium Analysis (ILASS Infinium Genotyping Assay v1.0)
Start Analysis v1.0
Trigger Location = Record Details
Trigger Style = Manual button
Step Data (Master Step Fields)
