Master Step Name = Extend and Stain BeadChip-Infinium v1.2
Step Type = No Outputs
Reagent Kits
Automations
Set Array Instrument
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Routing Script - iScan
Trigger Location = Step
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Naming Convention = {InputItemName}
Reagent Kits
1X TE
Lambda DNA
PicoGreen dsDNA quantification reagent
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Placement Pattern = Row
Destination Containers
96 well plate
Lambda serial dilution instruction notes
Multiline Text
Read Only
Default =
Add 66.7 uL 1X TE to well B1.
Add 100 µl 1X TE to the remaining wells of column 1.
Transfer 133.3 µl Lambda DNA from well A1 to well B1. Pipette up and down several times to mix.
Transfer 100 µl from well B1 to well C1. Pipette up and down several times to mix.
Repeat the sequential transfer of 100 µl for wells D1, E1, F1, and G1. Do not transfer from well G1 to H1. Well H1 serves as the blank 0 ng/µl Lambda DNA.
No. of QDNA Plates
Numeric Dropdown
Presets
1
2
PicoGreen (uL)
Numeric
Read Only
Sample QDNA Plate instruction notes
Multiline Text
Read Only
Default =
Transfer 195 μl PicoGreen/1xTE dilution to each sample well of the Sample QDNA plate.
Add 2 µl DNA sample to each well containing PicoGreen/1xTE
Standard QDNA Plate instruction notes
Multiline Text
Read Only
Default =
Transfer 195 µl PicoGreen/1X TE dilution from the Standard DNA plate to columns 1 and 2 of the Standard QDNA plate.
Transfer 2 µl stock Lambda DNA dilution from the Standard DNA plate to columns 1 and 2 of the Standard QDNA plate.
Stock Lambda DNA (ng/uL)
Numeric
Stock Lambda DNA for A1 (uL)
Numeric
Read Only
Step File Placeholders
Log - Automatically attached
Results File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Collapse
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Naming Convention = {InputItemName}
Reagent Kits
MA1-Infinium
Supplier = Illumina
Website =
MSM-Infinium
Supplier = Illumina
Website =
NaOH
RPM-Infinium
Supplier = Illumina
Website =
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Placement Pattern = Same Shape
Destination Containers
96 well plate
Default =
Add 20 µl MA1 to each well of the MSA3 plate.
Transfer 4 µl DNA sample from the DNA plate to the corresponding wells of the plate
Add 4 µl 0.1N NaOH in to each sample well of the plate
Add 68 μl RPM in to each sample well of the plate.
Add 75 μl MSM in to each sample well of the plate
Vortex at 1600 rpm for 1 minute, and then pulse centrifuge at 280 × g.
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Default = Incubate the plate in the Illumina Hybridization Oven for 20–24 hours at 37°C.
ℹ The workflow version and step version for the Array Instrument may vary depending on the version of the IPP.
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Derived Sample
DNA Plate Barcode
Text
Read Only
Derived Sample
Infinium Kit Name
Text
Read Only
Default =
Without allowing pipette tips to touch BeadChip surfaces, fill the reservoir of each flow-through chamber as follows:
150 µl RA1. Incubate for 30 seconds. Repeat 5 times.
225 µl LX1. Incubate for 10 minutes.Repeat once.
225 µl LX2. Incubate for 10 minutes. Repeat once.
300 µl EML. Incubate for 15 minutes.
250 µl 95% formamide/1 mM EDTA. Incubate for 1 minute. Repeat twice.Incubate 5 minutes.
Set the the chamber rack temperature to the temperature indicated on the SML tube.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Stain Instruction Notes
Multiline Text
Read Only
Default =
Fill the reservoir of each flow-through chamber as follows:
250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl ATM. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes. g 250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl ATM. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
Wash and Coat Beadships Instruction Notes
Multiline Text
Read Only
Default =
Add 310 ml PB1 to the PB1 wash dish.
Add 310 ml XC4 to the XC4 wash dish
