The Equalizer Workflow AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is part of the AmpliSeq for Illumina Transcriptome Human Gene Expression Panel protocols which include the following functionality:
Preconfigured AmpliSeq for Illumina Transcriptome Human Gene Expression Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of total RNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes. Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
There is no extraction protocol in this workflow because the samples are already extracted.
Protocol 1: Equalizer Workflow (AmpliSeq for Illumina Transcriptome Human Gene Expression Panel v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina Transcriptome Human Gene Expression Panel v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina Transcriptome Human Gene Expression Panel v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Automations
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Calculate Total Samples and Calculate Master Mix
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Group of Defaults
AmpliSeq for CHS V2
Thermal Cycler Program = AMP
Equalizer Workflow
Thermal Cycler Program = EQUAL
Standard Workflow
Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Equalizer Workflow
Master Step Fields
Step 3: Perform Capture and Clean Up (AmpliSeq for Illumina Transcriptome Human Gene Expression Panel v1.1)
Master Step Name = Perform Capture and Clean Up (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step File Placeholders
Log - Automatically attached
Sample Table
Step 4: Elute Library (AmpliSeq for Illumina Transcriptome Human Gene Expression Panel v1.1)
Master Step Name = Elute Library (AmpliSeq for Illumina v1.1)
