The integration includes the following features:

• Preconfigured NovaSeq X Series Sequencing v1.1 workflow that maps to lab protocols and instrument runs.

• The following steps in the NovaSeq X Series Sequencing v1.1 preconfigured protocol:

  • Assign Analysis Configuration Template (NovaSeq X Series Sequencing v1.1)

  • Make Bulk Pool (NovaSeq X Series Sequencing v1.1)

  • Dilute and Denature (NovaSeq X Series Sequencing v1.1)

  • Load to Library Tube Strip (NovaSeq X Series Sequencing v1.1)

  • AUTOMATED - Sequencing Run (NovaSeq X Series Sequencing v1.1)

  • AUTOMATED - Analysis Run (NovaSeq X Series Sequencing v1.1)

• Automatic validation of run setup information before sample sheet generation or planned run creation.

• Automated generation of the sample sheet. This sample sheet is used to start the sequencing run on the NovaSeq X Series Control Software through Local run mode.

• Automated creation of a planned run on Illumina Connected Analytics (ICA). The NovaSeq X Series Control Software retrieves the planned run for use with the Cloud run mode.

• Automated tracking of the NovaSeq X Series sequencing run and parsing of run statistics from BaseSpace Sequence Hub to Clarity LIMS.

• Automated tracking of the NovaSeq X Series analysis run and high level analysis summary from BaseSpace Sequence Hub to Clarity LIMS.

• Support for configuration of multiple secondary analyses with multiple library prep kits (LPKs) and index adapter kits (IAKs) for the planned run. The analysis is either performed by ICA in Cloud mode or by DRAGEN on-board the instrument in Local mode.

• [Optional] Preconfigured Library Prep Validation workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep required to produce libraries that are ready for the NovaSeq X Series Sequencing v1.1 workflow.

• [Optional] Clarity LIMS Product Analytics (CLPA) integration that can be used with ICA. The CLPA integration installation and configuration is required. For more information, refer to Clarity LIMS Product Analytics Integration v1.3.1 Configuration.

Last Updated: November 2024

Release Date: July 2024

Document Version: 2

These release notes describe the key changes to software components for the Clarity LIMS NovaSeq X Series Integration Package v1.2.1.

Prerequisites

• BaseSpace Sequence Hub account that has the Enterprise or Professional subscription

Compatibility

Refer to Compatibility under Instruments & Integrations.

Defects Fixed

• Fixed the issue of unable to generate sample sheet and planned run when any sample in the pools has been assigned QC flag prior to entering the Load to Library Tube Strip step.

Known Limitations

• The analysis step tracks only the first run, but not subsequent queued runs.

• The Assign Analysis Configuration Template step expects the input samples to be unpooled libraries.

Known Issues

• On the Make Bulk Pool step, the log displays a warning when the Calculate Volume automation is triggered and at least one pool consists of multiple inputs. This issue is caused by the output custom field being reset multiple times at the end of the automation. This issue does not affect the Calculat Volume automation functionality.

Documentations

The NovaSeq X Series Integration v1.2.1 guides follows exactly that of NovaSeq X Series Integration v1.2.0 with the following updates:

• NovaSeq X Series Integration v1.2.0 Configuration

  • NovaSeq X Series Integration Package version has been updated from v1.2.0 to v1.2.1.

• NovaSeq X Series Integration v1.2.0 User Interaction, Validation and Troubleshooting

  • NovaSeq X Series Integration Package version has been updated from v1.2.0 to v1.2.1.

Revision History