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iScan System v1.0

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Overview

The iScan workflow includes the following functionality:

  • Preset protocol.

  • Ability to attach the scan log to the samples.

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Protocol 1: Infinium Array Imaging (iScan v1.0)

Protocol Type = Other

Next Steps Configuration

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Step 1: Image Infinium BeadChip (iScan v1.0)

  • Master Step Name = Image Infinium BeadChip - iScan v1.0

  • Step Type = No Outputs

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Automations

chevron-rightSet Array Secondary Analysishashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightRouting Script - GenomeStudiohashtag
  • Trigger Location = Step

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Sample Table (Column Headers)

Category

Field Name

Field Type

Options

Additional Options and Dropdown Items

Container

Container Name

Built-in

Container

LIMS ID (Container)

Built-in

Step File Placeholders
  • Log - Automatically attached

  • Scan Log - Manually uploaded

  • Sample Table

    • Sample Display Default = Collapse

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -exp 'if (input.::Infinium Kit Name:: == ::Infinium MethylationEPIC Kit:: ) { input.::Array Secondary Analysis:: = ::GS-METHYLATION::} ; if (input.::Infinium Kit Name:: != ::Infinium MethylationEPIC Kit:: ) { input.::Array Secondary Analysis:: = ::GS-GENOTYPING::}' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {stepURI:v2} -l {compoundOutputFileLuid0} script:changeWorkflow \
    \
    --FIELD_NAME 'Array Secondary Analysis' \
    --FIELD_VALUE 'GS-GENOTYPING' \
    --WORKFLOW 'GenomeStudio v1.0' \
    --STEP 'Create Infinium Genotyping Analysis Sample Sheet (GenomeStudio v1.0)' \
    --INPUTS_OR_OUTPUTS 'INPUTS' \
    --FIELD_NAME 'Array Secondary Analysis' \
    --FIELD_VALUE 'GS-METHYLATION' \
    --WORKFLOW 'GenomeStudio v1.0' \
    --STEP 'Create Infinium Methylation Analysis Sample Sheet (GenomeStudio v1.0)' \
    --INPUTS_OR_OUTPUTS 'INPUTS'"

    BeadChip Position

    Text

    Read Only

    Derived Sample

    DNA Plate Barcode

    Text

    Read Only

    Derived Sample

    Infinium Kit Name

    Text

    Read Only

    Derived Sample

    Sample Name

    Built-in

    Derived Sample

    Sample_Well

    Text

    Project

    Project Name

    Built-in

    Container

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Derived Sample

    Waiting

    Built-in

    Project

    Project Name

    Built-in

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Derived Sample

    Array Secondary Analysis

    Text Dropdown

    • Presets

      • GS-GENOTYPING

      • GS-METHYLATION

    • Default = GS-GENOTYPING

    Derived Sample

    BeadChip Barcode

    Text

    Read Only

    Derived Sample