The Illumina RNA Prep with Enrichment (L) Tagmentation workflow includes the following functionality.
Preset Illumina RNA Prep with Enrichment (L) Tagmentation protocols that support the generation of enriched libraries for dual-indexed, paired-end sequencing.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Denature RNA v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Synthesize First Strand cDNA v1.0
Step Type = No Outputs
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Synthesize Second Strand cDNA v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP, 5 ml
Supplier = Beckman Coulter
Catalog Number = A63880
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Tagment cDNA v1.0
Step Type = No Outputs
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Amplify Tagmented cDNA v1.0
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}-{AppliedReagentLabels}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Column
Destination Containers
96 well plate
Label Groups
IDT for Illumina DNA-RNA UD Indexes Set A Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set B Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set C Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set D Tagmentation
Illumina DNA-RNA UD Indexes Set A Tagmentation
Illumina DNA-RNA UD Indexes Set B Tagmentation
Illumina DNA-RNA UD Indexes Set C Tagmentation
Illumina DNA-RNA UD Indexes Set D Tagmentation
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Clean Library (Illumina RNA Prep with Enrichment (L) Tagmentation v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP, 5 ml
Supplier = Beckman Coulter
Catalog Number = A63880
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Qubit Library QC v1.0
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
QC Result File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = BioAnalyzer Library QC v1.0
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Column
Destination Containers
BioAnalyzer DNA High Sensitivity Chip
BioAnalyzer DNA 1000 Chip
Step Data (Master Step Fields)
Step File Placeholders
Bioanalyzer Driver File - Automatically attached
Bioanalyzer Driver File Generation Log File - Automatically attached
Bioanalyzer XML Result File (required) - Manually uploaded
Result File (optional) - Manually uploaded
PDF Summary File (optional) - Manually uploaded
Bioanalyzer XML Parsing Log File - Automatically attached
QC Assignment Log File - Automatically attached
QC Assignment Report - Automatically attached
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Dilute Libraries v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Hybridize Probes (Illumina RNA Prep with Enrichment (L) Tagmentation v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina Exome Panel
Supplier = Illumina
Catalog Number = 20020183
Website = www.illumina.com
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Manually uploaded
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Capture Hybridize Probes v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Amplify Enriched Library v1.0
Step Type = No Outputs
Reagent Kits
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Manually uploaded
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Clean Up Enriched Library v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP, 5 ml
Supplier = Beckman Coulter
Catalog Number = A63880
Illumina RNA Prep with Enrichment (L) Tagmentation
Supplier = Illumina
Catalog Number = 16 - 20040536, 96 - 20040537
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Final Diluted Concentration (ng/uL)
Numeric
Required Field
Default = 10
Range = 10 To 100
Final Volume (uL)
Numeric
Default = 8.5
Instruction Notes
Multiline Text
Read Only
Default = Enter Final Diluted Concentration and Starting Concentration before clicking on the Calculate Sample and Water Volume button.
Thermal Cycler Program
Text
Default = DEN_RNA
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 17 µl 65°C for 5 minutes Hold at 4°C
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Nuclease-free Ultrapure Water (uL)
Numeric
Decimal Places Displayed = 0
Derived Sample
Sample Name
Built-in
Derived Sample
SampleVolume (uL)
Numeric
Decimal Places Displayed = 0
Derived Sample
Starting Concentration (ng/uL)
Numeric
Decimal Places Displayed = 0
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
FSA (uL)
Numeric
Decimal Places Displayed = 0
Instruction Notes
Multiline Text
Read Only
Default = Add 8 uL of First Strand Synthesis Master Mix to each sample.
RVT (uL)
Numeric
Decimal Places Displayed = 0
Thermal Cycler Program
Text
Default = FSS
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 25 µl 25°C for 10 minutes 42°C for 15 minutes 70°C for 15 minutes Hold at 4°C
Total Samples
Numeric
Default = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Thermal Cycler Program
Text
Default = SSS
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 40°C Reaction volume is 50 µl u 16°C for 1 hour Hold at 4°C
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
EBLTL (uL)
Numeric
Instruction Notes
Multiline Text
Read Only
Default = Add 32.5 µl Tagmentation Master Mix to each well.
Nuclease-free ultrapure water (uL)
Numeric
TB1 (uL)
Numeric
Thermal Cycler Program
Text
Default = TAG
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 50 µl u 55°C for 5 minutes Hold at 10°C
Total Samples
Numeric
Default = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
EPM (uL)
Numeric
Decimal Places Displayed = 0
Instruction Notes
Multiline Text
Read Only
Default = Add 40 µl PCR Master Mix to each well.
Nuclease-free ultrapure water (uL)
Numeric
Decimal Places Displayed = 0
PCR Cycles
Text Dropdown
Custom Entries
Presets
14 cycles for High-quality RNA with DV200 > 80%
17 cycles for FFPE and RNA with DV200 < 80%
Thermal Cycler Program
Text
Default = TAG_PCR
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 50 µl u 72°C for 3 minutes u 98°C for 3 minutes X cycles of: u 98°C for 20 seconds u 60°C for 30 seconds 72°C for 1 minute u 72°C for 3 minutes Hold at 10°C
Total Samples
Numeric
Default = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration
Criteria 1 - Threshold Value
Numeric
Default = 200
Criteria 2 - Operator
Text
Default = <=
Criteria 2 - Source Data Field
Text
Default = Concentration
Criteria 2 - Threshold Value
Numeric
Default = 1500
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Sample Name
Built-in
Measurement
Concentration
Numeric
Decimal Places Displayed = 2
Measurement
Conc. Units
Text
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Criteria 1 - Source Data Field
Text Dropdown
Custom Entries
Presets
Peak 2 Size - bp
Peak 2 Conc.
Peak 2 Molarity
Criteria 1 - Threshold
Numeric
Criteria 2 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Criteria 2 - Source Data Field
Text Dropdown
Custom Entries
Presets
Peak 2 Size - bp
Peak 2 Conc.
Peak 2 Molarity
Criteria 2 - Threshold
Numeric
Use strict matching for Bioanalyzer results
Toggle Switch
Default = None Set
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Enrichment Plexity
Numeric Dropdown
Required Field
Custom Entries
Presets
1
3
Final Diluted Concentration (ng/uL)
Numeric
Default = 200
Instruction Notes
Multiline Text
Read Only
Default = In one well of a new PCR plate, combine the applicable number of 200 ng libraries: If Enrichment Plexity is 1 then add 7.5 ul of the sample. If Enrichment Plexity is 3 then add 2.5 ul of each sample to one well with a final volume of 7.5 ul.
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Final Volume (uL)
Numeric
Decimal Places Displayed = 0
Derived Sample
RSB (uL)
Numeric
Decimal Places Displayed = 0
Derived Sample
Sample Name
Built-in
Derived Sample
SampleVolume (uL)
Numeric
Decimal Places Displayed = 0
Derived Sample
Starting Concentration (ng/uL)
Numeric
Decimal Places Displayed = 0
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Thermal Cycler Program
Text
Default = HYB
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 25 µl u 95°C for 5 minutes 18 cycles of 1 minute each: u 94°C for the first cycle Decrease 2°C per subsequent cycle 58°C for 90 minutes Hold at 58°C for ≤ 24 hours
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
EEL (uL)
Numeric
Decimal Places Displayed = 0
HP3 (uL)
Numeric
Decimal Places Displayed = 0
Instruction Notes
Multiine Text
Read Only
Default = Add 23 µl Elution Master Mix to each well.
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 70°C Reaction volume is 100 µl Hold at 58°C
Total Samples
Numeric
Default = 0
Decimal Places Displayed = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Thermal Cycler Program
Text
Default = AMP
Thermal Cycler Program Notes
Multiline Text
Read Only
Default = Choose the preheat lid option and set to 100°C Reaction volume is 50 µl 98°C for 30 seconds 14 cycles of: 98°C for 10 seconds 60°C for 30 seconds 72°C for 30 seconds 72°C for 5 minutes Hold at 10°C
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Sort Sample (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = No Outputs
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Blood Lysis (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP beads, 5 ml
Supplier = Beckman Coulter
Catalog Number = A63880
Website = www.beckman.com
Flex Lysis Reagent Kit
Supplier = Illumina
Catalog Number = 20015884
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Saliva Lysis (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP, 5 ml
Supplier = Beckman Coulter
Catalog Number = A63880
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Qubit gDNA QC (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
QC Log File - Automatically attached
QC Result File - Automatically attached
Upload File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Dilute gDNA (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Qubit FFPE DNA QC (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
QC Log File - Automatically attached
QC Result File - Automatically attached
Upload File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Dilute FFPE DNA (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Tagment Genomic DNA (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina DNA Prep -Tagmentation (S) Beads
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Illumina DNA/RNA Prep - Tagmentation PCR Reagents
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Post Tagmentation Cleanup (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = No Outputs
Reagent Kits
Illumina DNA/RNA Prep - Tagmentation Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Amplify Tagmented DNA (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina DNA Fast Hyb - Enrichment PCR + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Label Groups
IDT for Illumina DNA-RNA UD Indexes Set A Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set A-D Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set B Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set C Tagmentation
IDT for Illumina DNA-RNA UD Indexes Set D Tagmentation
Illumina DNA-RNA UD Indexes Set A B C D Tagmentation
Illumina DNA-RNA UD Indexes Set A Tagmentation
Illumina DNA-RNA UD Indexes Set B Tagmentation
Illumina DNA-RNA UD Indexes Set C Tagmentation
Illumina DNA-RNA UD Indexes Set D Tagmentation
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Clean Up Libraries (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP Beads
Supplier = Thermo
Catalog Number = NC9959336; NC9933872
Illumina DNA Prep -Tagmentation (S) Beads
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Bioanalyzer QC Qualify Pre-Enriched Libraries (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
BioAnalyzer DNA High Sensitivity Chip
BioAnalyzer DNA 1000 Chip
Step Data (Master Step Fields)
Step File Placeholders
Bioanalyzer Input File - Automatically attached
Bioanalyzer Input File Generation Log File - Automatically attached
Bioanalyzer XML Result File (required) - Manually uploaded
Result File (optional) - Manually uploaded
PDF Summary File (optional) - Manually uploaded
Bioanalyzer XML Parsing Log File - Automatically attached
QC Assignment Log File - Automatically attached
QC Assignment Report - Automatically attached
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Show
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Pool Pre-Enriched Libraries (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Pooling
Aliquot Generation = Fixed, 1
Naming Convention = {PoolName}
Reagent Kits
Agencourt AMPure XP Beads
Supplier = Thermo
Catalog Number = NC9959336; NC9933872
Illumina DNA Prep -Tagmentation (S) Beads
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Label Uniqueness = On
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Defaults
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Qubit (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
QC Log File - Automatically attached
QC Result File - Automatically attached
Upload File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Master Step Name = Pool Pre-Enriched Libraries - Mass (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Pooling
Aliquot Generation = Fixed, 1
Naming Convention = {PoolName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Label Uniqueness = On
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Defaults
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Hybridize Probes (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina DNA Fast Hyb - Enrichment Beads + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Illumina DNA Fast Hyb - Enrichment PCR + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Capture Hybridize Probes (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina DNA Fast Hyb - Enrichment Beads + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Illumina DNA Fast Hyb - Enrichment PCR + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Amplify Enriched Library (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Illumina DNA Fast Hyb - Enrichment PCR + Buffers
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Clean Up Amplified Enriched Library (Illumina DNA Prep with Enrichment (S) Tagmentation v1.2)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Agencourt AMPure XP Beads
Supplier = Thermo
Catalog Number = NC9959336; NC9933872
Illumina DNA Prep -Tagmentation (S) Beads
Supplier = Illumina
Catalog Number = 20025519; 20025520
Website = illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
The version of the workflow and step for the routing script may be different depending on the version of IPP installed.
The version of the workflow and step for the routing script may be different depending on the version of IPP installed.
The version of the workflow and step for the routing script may be different depending on the version of IPP installed.
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
NFE Sample Type
Text Dropdown
Required Field
Presets
Blood
Saliva
gDNA
FFPE DNA
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
BLB (uL)
Numeric
Decimal Places Displayed = 2
PK1 (uL)
Numeric
Decimal Places Displayed = 2
Thermal Cycler Program
Text
Default = BLP
Total Samples
Numeric
Default = 0
Water (uL)
Numeric
Decimal Places Displayed = 2
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
80% EtOH (uL)
Numeric
Derived Sample
NFE Sample Type
Text Dropdown
Required Field
Presets
Blood
Saliva
gDNA
FFPE DNA
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (uL)
Numeric
Decimal Places Displayed = 2
Derived Sample
Water for Resuspension (uL)
Numeric
Default = 30
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration
Criteria 1 - Threshold Value
Numeric
Criteria 2 - Operator
Text
Default = <=
Criteria 2 - Source Data Field
Text
Default = Concentration
Criteria 2 - Threshold Value
Numeric
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Sample Name
Built-in
Measurement
Concentration
Numeric
Decimal Places Displayed = 2
Measurement
Conc. Units
Text
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Desired gDNA Concentration (ng/uL)
Numeric
Decimal Places Displayed = 2
Dilution Buffer
Text Dropdown
Custom Entries
Presets
RSB
10 mM Tris-HCI, pH 7.5–8.5
Total Volume (uL)
Numeric
Decimal Places Displayed = 2
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Dilution Volume (uL)
Numeric
Derived Sample
Final Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (uL)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration
Criteria 1 - Threshold Value
Numeric
Criteria 2 - Operator
Text
Default = <=
Criteria 2 - Source Data Field
Text
Default = Concentration
Criteria 2 - Threshold Value
Numeric
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Sample Name
Built-in
Measurement
Concentration
Numeric
Decimal Places Displayed = 2
Measurement
Conc. Units
Text
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Desired gDNA Concentration (ng/uL)
Numeric
Decimal Places Displayed = 2
Dilution Buffer
Text Dropdown
Custom Entries
Presets
RSB
10 mM Tris-HCI, pH 7.5–8.5
Total Volume (uL)
Numeric
Decimal Places Displayed = 2
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Dilution Volume (uL)
Numeric
Derived Sample
Final Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (uL)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Desired Input Amount (ng)
Numeric
Range = 50 To 1000
eBLT (uL)
Numeric
Notes
Multiline Text
Default = Desired Input Amount should be between 50-1000 ng.
TB1 (uL)
Numeric
Thermal Cycler Instructions
Multiline Text
Default = Choose the preheat lid option and set to 100C Set the reaction volume to 50 uL 55C for 5 minutes Hold at 10C
Thermal Cycler Program
Text
Default = TAG
Total Samples
Numeric
Default = 0
Total Volume (uL)
Numeric
Default = 30
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Desired Input Amount (ng)
Numeric
Derived Sample
NFE Sample Type
Text Dropdown
Required Field
Presets
Blood
Saliva
gDNA
FFPE DNA
Derived Sample
Nuclease-free water (uL)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (uL)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
NFE Sample Type
Text Dropdown
Required Field
Presets
Blood
Saliva
gDNA
FFPE DNA
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
EPM (uL)
Numeric
Nuclease-free water (uL)
Numeric
Thermal Cycler Program
Text
Default = eBLT PCR
Thermal Cycler Program Instructions
Multiline Text
Default = Choose the preheat lid option and set to 100°C Set the reaction volume to 50 uL 72C for 3 minutes 98C for 3 minutes X cycles of: X = 98C for 20 seconds X = 0C for 30 seconds X = 72C for 1 minute 72C for 3 minutes Hold at 10C
Total Samples
Numeric
Default = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
NFE Sample Type
Text Dropdown
Required Field
Presets
Blood
Saliva
gDNA
FFPE DNA
Derived Sample
PCR Cycles
Numeric
Decimal Places Displayed = 0
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
80% EtOH Prep Date
Date
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text
Default = >=
Criteria 1 - Source Data Field
Text
Default = Peak 2 Size - bp
Criteria 1 - Threshold Value
Numeric
Default = 300
Criteria 2 - Operator
Text
Default = <=
Criteria 2 - Source Data Field
Text
Default = Peak 2 Size - bp
Criteria 1 - Threshold Value
Numeric
Default = 400
Use strict matching for Bioanalyzer results
Toggle Switch
Default = None Set
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Measurement
A260/280 ratio
Numeric
Decimal Places Displayed = 2
Measurement
Number of Peaks found
Numeric
Decimal Places Displayed = 0
Measurement
Number of Regions found
Numeric
Decimal Places Displayed = 0
Measurement
Peak 1 % Integrated Area
Numeric
Decimal Places Displayed = 2
Measurement
Peak 1 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Peak 1 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Peak 1 MW
Numeric
Decimal Places Displayed = 0
Measurement
Peak 1 Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Peak 2 % Integrated Area
Numeric
Decimal Places Displayed = 2
Measurement
Peak 2 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Peak 2 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Peak 2 MW
Numeric
Decimal Places Displayed = 0
Measurement
Peak 2 Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Peak 3 % Integrated Area
Numeric
Decimal Places Displayed = 2
Measurement
Peak 3 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Peak 3 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Peak 3 MW
Numeric
Decimal Places Displayed = 0
Measurement
Peak 3 Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Peak 4 % Integrated Area
Numeric
Decimal Places Displayed = 2
Measurement
Peak 4 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Peak 4 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Peak 4 MW
Numeric
Decimal Places Displayed = 0
Measurement
Peak 4 Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Peak 5 % Integrated Area
Numeric
Decimal Places Displayed = 2
Measurement
Peak 5 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Peak 5 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Peak 5 MW
Numeric
Decimal Places Displayed = 0
Measurement
Peak 5 Size - bp
Numeric
Decimal Places Displayed = 2
Measurement
Region 1 % of Total
Numeric
Decimal Places Displayed = 2
Measurement
Region 1 Average Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Region 1 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Region 1 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Region 2 % of Total
Numeric
Decimal Places Displayed = 2
Measurement
Region 2 Average Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Region 2 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Region 2 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Region 3 % of Total
Numeric
Decimal Places Displayed = 2
Measurement
Region 3 Average Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Region 3 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Region 3 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Region 4 % of Total
Numeric
Decimal Places Displayed = 2
Measurement
Region 4 Average Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Region 4 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Region 4 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Region 5 % of Total
Numeric
Decimal Places Displayed = 2
Measurement
Region 5 Average Size - bp
Numeric
Decimal Places Displayed = 0
Measurement
Region 5 Conc.
Numeric
Decimal Places Displayed = 2
Measurement
Region 5 Molarity
Numeric
Decimal Places Displayed = 2
Measurement
Sample Comment
Text
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Library Pool Plexity
Text Dropdown
Required Field
Custom Entries
Presets
1-plex
12-plex
Total Pooled Volume (uL)
Numeric
Read Only
Default = 30
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Pre-Enriched Library Volume (uL)
Numeric
Derived Sample
RSB Volume (uL)
Numeric
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration
Criteria 1 - Threshold Value
Numeric
Criteria 2 - Operator
Text
Default = <=
Criteria 2 - Source Data Field
Text
Default = Concentration
Criteria 1 - Threshold Value
Numeric
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Measurement
Concentration
Numeric
Decimal Places Displayed = 2
Measurement
Conc. Units
Text
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
Library Pool Plexity
Text Dropdown
Required Field
Presets
1-plex
12-plex
Pre-Enriched Library Sample (ng)
Numeric
Read Only
Default = 500
Total Pooled Volume (uL)
Numeric
Read Only
Default = 30
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration
Numeric
Required Field
Decimal Places Displayed = 2
Derived Sample
Conc. Units
Text
Required Field
Derived Sample
Pre-Enriched Library Volume (uL)
Numeric
Derived Sample
RSB Volume (uL)
Numeric
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
PCR Cycles
Numeric
Thermal Cycler Program
Text
Default = NF-HYB program
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
EE1 (uL)
Numeric
Decimal Places Displayed = 2
HP3 (uL)
Numeric
Decimal Places Displayed = 2
Total Samples
Numeric
Default = 0
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Field Name
Field Type
Options
Additional Options and Dropdown Items
PCR Cycles
Numeric
Thermal Cycler Program
Text
Default = AMP
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Project
Project Name
Built-in
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in