Normalization Buffer Volumes

Available from: Clarity LIMS v1.0

This section discusses the normalizationBufferVolumes script, which is used to calculate buffer volumes.

Script Overview

The normalizationBufferVolumes script generates a comma-separated file — Normalization buffer volumes.csv — file that holds the calculated buffer volumes information. The generated file is a attached to the Library Normalization step in the LIMS.

The script calculates the 'Volume of Dilution Buffer (uL)' as follows:

• "Volume of Dilution Buffer (uL)" = ( ("Source Volume (uL)" * "Source Conc.") / "Destination Conc." ) - "Source Volume (uL)"

Script Parameters

Command Line Example

Configuration

The following step UDFs are configured on the Library Normalization step:

• Library volume (ul) transferred to DESTINATION plate: Numeric, Default = 10

• Normalized conc. (nM): Numeric, Default = 2

• Maximum destination container volume (ul): Numeric, Default = 800

The inputs to the step must have values for the following sample UDFs:

• Concentration: Numeric

• Conc. Units: Single Line Text

Rules & constraints

• One sample input generates one sample output and one shared result file.

• The CSV file header contains the date and a record of the user who generated the file.

• The contents of the CSV file are ordered by source plate and then source well.

Additional information

Other scripts you may find useful: