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Illumina Lab Automation Software Solution (iLASS) Infinium Genotyping v1.1 consists of three workflows:
iLASS Infinium Genotyping Assay v1.1
iLASS Infinium Genotyping Assay with PGx v1.1
iLASS Infinium Batch DNA v1.1
The iLASS Infinium Batch DNA v1.1 workflow is a single-step protocol that supports preparation of automated cherry-picking of samples.
iLASS Infinium Genotyping Assay v1.1 and iLASS Infinium Genotyping Assay with PGx v1.1 workflows, each includes a preconfigured protocol with the following functionality:
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step of the protocol.
Automatic step transition when required.
Automatic placement of samples when required.
Automated assignment of QC Pass/Fail based on the user-selected threshold values.
A routing script that allows library sequencing with any Illumina instrument.
Illumina Lab Automation Software Solution (iLASS) Infinium Genotyping v1.0 consists of two workflows:
iLASS Infinium Genotyping Assay v1.0
iLASS Infinium Genotyping Assay with PGx v1.0
Each workflow includes a preconfigured protocol with the following functionality:
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step of the protocol.
Automatic step transition when required.
Automatic placement of samples when required.
Automated assignment of QC Pass/Fail based on the user-selected threshold values.
A routing script that allows library sequencing with any Illumina instrument.
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Make WGA (ILASS Infinium Genotyping Assay v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Container
Master Step Name = Amplify WGA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Fragment DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
FMS-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Precipitate DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
PM1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Resuspend DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
IHX-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Prepare Hyb Chamber (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB2-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Hybridize DNA to the BeadChip (ILASS Infinium Genotyping Assay v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
Infinium Genotyping Array EX-24
Infinium Genotyping Array EX-48
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Wash BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Extend and Stain BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
ATX-Infinium
Supplier = Illumina
EML-Infinium
Supplier = Illumina
LX1-Infinium
Supplier = Illumina
LX2-Infinium
Supplier = Illumina
PB1-Infinium
Supplier = Illumina
STX-Infinium
Supplier = Illumina
XC3-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Wash and Coat BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
XC4-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Other
Next Steps Configuration
Master Step Name = Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay v1.1)
Step Type = Analysis
Derived Sample Generation = Fixed, 1
Instrument Types
iScan
Vendor = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Scan Log - Manually uploaded
Run Metrics File - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Measurement
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Invoke Infinium Analysis (ILASS Infinium Genotyping Assay v1.1)
Step Type = Analysis
Derived Sample Generation = Fixed, 1
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Analysis Report - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Batch DNA (ILASS Infinium Batch DNA v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Plate Manifest - Manually uploaded
Log - Automatically attached
Plate Transfer Plan - Manually uploaded
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name: Make WGA and PGx (ILASS Infinium Genotyping Assay v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 2
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
MSM-Infinium
Supplier = Illumina
PGM-Infinium
Supplier = Illumina
PGP-Infinium
Supplier = Illumina
RPM-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Container
Master Step Name = Amplify WGA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Amplify PGx (ILASS Infinium Genotyping Assay with PGx v1.1)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Recombine and Fragment DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = Pooling
Aliquot Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
FMS-Infinium
Supplier = Illumina
Label Uniqueness = Off
Sample Grouping = Group by Containers
Well Sort Order = Row
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Container
Master Step Name = Precipitate DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
PM1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Resuspend DNA (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
IHX-Infinium
Supplier = Illumina
RA1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Prepare Hyb Chamber (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB2-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Hybridize DNA to the BeadChip (ILASS Infinium Genotyping Assay v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
Infinium Genotyping with PGx Array LCG-8
Infinium Genotyping with PGx Array EX-24
Infinium Genotyping with PGx Array EX-48
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Wash BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Extend and Stain BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
ATM-Infinium
Supplier = Illumina
ATX-Infinium
Supplier = Illumina
EML-Infinium
Supplier = Illumina
LX1-Infinium
Supplier = Illumina
LX2-Infinium
Supplier = Illumina
PB1-Infinium
Supplier = Illumina
RA1-Infinium
Supplier = Illumina
SML-Infinium
Supplier = Illumina
STX-Infinium
Supplier = Illumina
XC3-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Wash and Coat BeadChips (ILASS Infinium Genotyping Assay v1.1)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
XC4-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Other
Next Steps Configuration
Master Step Name = Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay v1.1)
Step Type = Analysis
Derived Sample Generation = Fixed, 1
Instrument Types
iScan
Vendor = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Scan Log - Manually uploaded
Run Metrics File - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Measurement
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Invoke Infinium Analysis (ILASS Infinium Genotyping Assay v1.1)
Step Type = Analysis
Derived Sample Generation = Fixed, 1
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Analysis Report - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Select First Workflow Step (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Batch DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standards
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Plate Manifest - Manually uploaded
Log - Automatically attached
Plate Transfer Plan - Manually uploaded
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name: Make WGA (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Container
Master Step Name = Make WGA (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Amplify WGA Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Fragment DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
FMS-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Precipitate DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
PMX-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Resuspend DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
IBX-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Hybridize DNA to the BeadChip Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
Infinium Genotyping Array EX-24
Infinium Genotyping Array EX-48
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Prepare Hyb Chamber Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB2-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Wash BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Extend and Stain BeadChips Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
ATX-Infinium
Supplier = Illumina
EML-Infinium
Supplier = Illumina
LX1-Infinium
Supplier = Illumina
LX2-Infinium
Supplier = Illumina
PB1-Infinium
Supplier = Illumina
STX-Infinium
Supplier = Illumina
XC3-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Wash and Coat BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
XC4-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Other
Next Steps Configuration
Master Step Name = Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
iScan
Vendor = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Invoke Infinium Analysis (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table (Global Step Fields)
Derived Sample
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Related Source Container
Text
Related WGA Container
Text
Step Run History
Multiline Text
Experiment Name
Text
Read Only
Product Type
Text
Read Only
Last Run Datetime
Text
Read Only
IsInProgress
Toggle Switch
Read Only
Default = No
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Incubate the WGA plate in the Illumina Hybridization Oven for 20–24 hours at 37°C.
Field Name
Field Type
Options
Additional Options and Dropdown Items
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
100% 2-Propanol Prep Date
Date
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
IsRecombined
Toggle Switch
Default = No
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Add 400 µl PB2 to the top and bottom wells of each beadchip subchamber in the hybridization chamber.
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Fill two wash dish with 200ml PB1 each and labelled Wash 1 and Wash2
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
IsRecombined
Toggle Switch
Default = No
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Add 310 ml PB1 to the PB1 wash dish. Add 310 ml XC4 to the XC4 wash dish.
Field Name
Field Type
Options
Additional Options and Dropdown Items
Array Instrument
Text
Default = iScan
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Barcode
Text
Read Only
Instrument Type
Text
Read Only
Instrument
Text
Read Only
Instrument Run ID
Text
Read Only
Run Start Time
Text
Read Only
Run Complete Time
Text
Read Only
Run Status
Text
Read Only
Failure Reason
Text
Read Only
Comment
Multiline Text
Log
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Name
Text
Read Only
Sample Section
Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Imaging Result
Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Analysis Configuration ID
Text
Required Field
Analysis Run Name
Text
Required Field
Analysis Start Time
Text
Read Only
Analysis Complete Time
Text
Read Only
Analysis Status
Text
Read Only
Log
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Name
Text
Read Only
Sample Section
Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Plating Source Barcode
Text
Plating Source Well
Text
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Related Source Container | Text |
Related PGX Container | Text |
Related WGA Container | Text |
Step Run History | Multiline Text |
Experiment Name | Text | Read Only |
Product Type | Text | Read Only |
Last Run Datetime | Text | Read Only |
IsInProgress | Toggle Switch | Read Only | Default = No |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Incubate the WGA plate in the Illumina Hybridization Oven for 20–24 hours at 37°C. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Thermocycler Start Time | Text |
Thermocycler End Time | Text |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Incubate the WGA plate in the Illumina Hybridization Oven for 20–24 hours at 37°C. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Related Input Containers | Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
100% 2-Propanol Prep Date | Date |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
IsRecombined | Toggle Switch | Default = No |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Add 400 µl PB2 to the top and bottom wells of each beadchip subchamber in the hybridization chamber. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Fill two wash dish with 200ml PB1 each and labelled Wash 1 and Wash2 |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
IsRecombined | Toggle Switch | Default = No |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Add 310 ml PB1 to the PB1 wash dish. Add 310 ml XC4 to the XC4 wash dish. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Array Instrument | Text | Default = iScan |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Barcode | Text | Read Only |
Instrument Type | Text | Read Only |
Instrument | Text | Read Only |
Instrument Run ID | Text | Read Only |
Run Start Time | Text | Read Only |
Run Complete Time | Text | Read Only |
Run Status | Text | Read Only |
Failure Reason | Text | Read Only |
Comment | Multiline Text |
Log | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Name | Text | Read Only |
Sample Section | Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Imaging Result | Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Analysis Configuration ID | Text | Required Field |
Analysis Run Name | Text | Required Field |
Analysis Start Time | Text | Read Only |
Analysis Complete Time | Text | Read Only |
Analysis Status | Text | Read Only |
Log | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Name | Text | Read Only |
Sample Section | Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
First Workflow Step Options | Text Dropdown | Required Field | Presets
|
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Plating Source Barcode | Text |
Plating Source Well | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Related Source Container | Text |
Related WGA Container | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Incubate the WGA plate in the Illumina Hybridization Oven for 20–24 hours at 37°C. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
100% 2-Propanol Prep Date | Date |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
IsRecombined | Toggle Switch | Default = No |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Plate Transfer Plan | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Add 400 µl PB2 to the top and bottom wells of each beadchip subchamber in the hybridization chamber. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Fill two wash dish with 200ml PB1 each and labelled Wash 1 and Wash2 |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
IsRecombined | Toggle Switch | Default = No |
Source DNA Plate Barcode | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Batch ID | Text | Read Only |
Comment | Multiline Text |
Instruction Notes | Multiline Text | Read Only | Default = Add 310 ml PB1 to the PB1 wash dish. Add 310 ml XC4 to the XC4 wash dish. |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Barcode | Text | Read Only |
Instrument Type | Text | Read Only |
Instrument | Text | Read Only |
Instrument Run ID | Text | Read Only |
Run Start Time | Text | Read Only |
Run Complete Time | Text | Read Only |
Run Status | Text | Read Only |
Failure Reason | Text | Read Only |
Log | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Name | Text | Read Only |
Sample Section | Text | Read Only |
Imaging Result | Text |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Analysis Configuration ID | Text | Required Field |
Analysis Run Name | Text | Required Field |
Analysis Start Time | Text | Read Only |
Analysis Complete Time | Text | Read Only |
Analysis Status | Text | Read Only |
Log | Multiline Text | Read Only |
Field Name | Field Type | Options | Additional Options and Dropdown Items |
BeadChip Name | Text | Read Only |
Sample Section | Text | Read Only |
Analysis Result | Text | Read Only |
The following global fields are used by the workflow. If these fields are already defined, they do not need to be added again.
From Configuration, select the Custom Fields tab.
Under Global Fields, select Container.
Select Product Type.
Under Field Type, select Text.
Under Field Options, make the following selections:
Required Field — No
Read Only — Yes
Select Step Run History.
Under Field Type, select Multiline Text.
Under Field Options, make the following selections:
Required Field — No
Read Only — No
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Select First Workflow Step with PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name: Batch DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standards
Derived Sample Generation = Fixed, 1
Instrument Types
Hamilton Star
Vendor = Hamilton
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Plate Manifest - Manually uploaded
Log - Automatically attached
Plate Transfer Plan - Manually uploaded
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Make WGA and PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 2
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
MSM-Infinium
Supplier = Illumina
PGM-Infinium
Supplier = Illumina
PGP-Infinium
Supplier = Illumina
RPM-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Container
Master Step Name = Make WGA and PGx (ILASS Infinium Genotyping Assay with PGx v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 2
Instrument Types
Hamilton Star
Vendor = Hamilton
Reagent Kits
AAX-Infinium
Supplier = Illumina
MA1-Infinium
Supplier = Illumina
MA2-Infinium
Supplier = Illumina
MSM-Infinium
Supplier = Illumina
PGM-Infinium
Supplier = Illumina
PGP-Infinium
Supplier = Illumina
RPM-Infinium
Supplier = Illumina
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Same Shape
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Amplify WGA Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Amplify PGx Manual (ILASS Infinium Genotyping Assay with PGx v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Recombine and Fragment DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = Pooling
Aliquot Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
FMS-Infinium
Supplier = Illumina
Label Uniqueness = Off
Sample Grouping = Group by Containers
Well Sort Order = Row
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Precipitate DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
PM1-Infinium
Supplier = Illumina
PMX-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Resuspend DNA Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
IBX-Infinium
Supplier = Illumina
RA1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Hybridize DNA to the BeadChip Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Instrument Types
Tecan EVO
Vendor = Tecan
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
Infinium Genotyping with PGx Array LCG-8
Infinium Genotyping with PGx Array EX-24
Infinium Genotyping with PGx Array EX-48
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Prepare Hyb Chamber Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB2-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Wash BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Master Step Name = Extend and Stain BeadChips Automated (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
Tecan EVO
Vendor = Tecan
Reagent Kits
ATM-Infinium
Supplier = Illumina
ATX-Infinium
Supplier = Illumina
EML-Infinium
Supplier = Illumina
LX1-Infinium
Supplier = Illumina
LX2-Infinium
Supplier = Illumina
PB1-Infinium
Supplier = Illumina
RA1-Infinium
Supplier = Illumina
SML-Infinium
Supplier = Illumina
STX-Infinium
Supplier = Illumina
XC3-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Sample Table (Global Step Fields)
Derived Sample
Master Step Name = Wash and Coat BeadChips Manual (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Reagent Kits
PB1-Infinium
Supplier = Illumina
XC4-Infinium
Supplier = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
AssayRunLog - Manually uploaded
Protocol Type = Other
Next Steps Configuration
Master Step Name = Image Infinium BeadChip on iScan (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Instrument Types
iScan
Vendor = Illumina
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table (Global Step Fields)
Derived Sample
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Invoke Infinium Analysis (ILASS Infinium Genotyping Assay v1.0)
Step Type = No Outputs
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table (Global Step Fields)
Derived Sample
Field Name
Field Type
Options
Additional Options and Dropdown Items
First Workflow Step Options with PGx
Text Dropdown
Required Field
Presets
Run Make WGA and PGx Step Manually
Run Batch DNA Step on Hamilton
Run Make WGA and PGx Step on Hamilton
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Plating Source Barcode
Text
Plating Source Well
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Related Source Container
Text
Related PGX Container
Text
Related WGA Container
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Incubate the WGA plate in the Illumina Hybridization Oven for 20–24 hours at 37°C.
Field Name
Field Type
Options
Additional Options and Dropdown Items
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Thermocycler Start Time
Text
Thermocycler End Time
Text
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Place the PGx plate in the thermal cycler and start the cycling program
Field Name
Field Type
Options
Additional Options and Dropdown Items
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
100% 2-Propanol Prep Date
Date
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
IsRecombined
Toggle Switch
Default = No
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Plate Transfer Plan
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Add 400 µl PB2 to the top and bottom wells of each beadchip subchamber in the hybridization chamber.
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Fill two wash dish with 200ml PB1 each and labelled Wash 1 and Wash2
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
IsRecombined
Toggle Switch
Default = No
Source DNA Plate Barcode
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Batch ID
Text
Read Only
Comment
Multiline Text
Instruction Notes
Multiline Text
Read Only
Default = Add 310 ml PB1 to the PB1 wash dish. Add 310 ml XC4 to the XC4 wash dish.
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Barcode
Text
Read Only
Instrument Type
Text
Read Only
Instrument
Text
Read Only
Instrument Run ID
Text
Read Only
Run Start Time
Text
Read Only
Run Complete Time
Text
Read Only
Run Status
Text
Read Only
Failure Reason
Text
Read Only
Log
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Name
Text
Read Only
Sample Section
Text
Read Only
Imaging Result
Text
Field Name
Field Type
Options
Additional Options and Dropdown Items
Analysis Configuration ID
Text
Required Field
Analysis Run Name
Text
Required Field
Analysis Start Time
Text
Read Only
Analysis Complete Time
Text
Read Only
Analysis Status
Text
Read Only
Log
Multiline Text
Read Only
Field Name
Field Type
Options
Additional Options and Dropdown Items
BeadChip Name
Text
Read Only
Sample Section
Text
Read Only
Analysis Result
Text
Read Only