Equalizer v1.1
Protocol 1: Equalizer Workflow (AmpliSeq for Illumina Focus Panel v1.1)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina Focus Panel v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina Focus Panel v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Calculate Total Samples and Calculate Master Mix
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Group of Defaults
AmpliSeq for CHS V2
Thermal Cycler Program = AMP
Equalizer Workflow
Thermal Cycler Program = EQUAL
Standard Workflow
Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Equalizer Workflow
Step 3: Perform Capture and Clean Up (AmpliSeq for Illumina Focus Panel v1.1)
Master Step Name = Perform Capture and Clean Up (AmpliSeq for Illumina v1.1)
Derived Sample Generation = Fixed, 1
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step File Placeholders
Log - Automatically attached
Step 4: Elute Library (AmpliSeq for Illumina Focus Panel v1.1)
Master Step Name = Elute Library (AmpliSeq for Illumina v1.1)
Derived Sample Generation = Fixed, 1
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
