TruSeq Methyl Capture EPIC v2.0 Protocol 1: TruSeq Methyl Capture EPIC v2.0
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Fragment DNA (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Fragment DNA (TruSeq Methyl Capture EPIC v2.0)
Derived Sample Generation = Fixed, 1
chevron-right Calculate Master Mixhashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Normalize gDNAhashtag Trigger Location = Record Details
Trigger Style = Manual button
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Bioanalyzer QC (DNA) (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Bioanalyzer QC (DNA) 5.1.2
Measurement Generation = Fixed, 1
chevron-right Generate Bioanalyzer driver filehashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Parse Bioanalyzer XML and assign QC flagshashtag Trigger Location = Record Details
Trigger Style = Manual button
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Set Next Step - Output PASS/FAILhashtag Trigger Location = Not Used
chevron-right Set Next Step and Copy to Inputhashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Group of Defaults
chevron-right NRCChashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right Peak 2 Size Thresholdshashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq ChIP-Seqhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Molarity
chevron-right TruSeq Methyl Capture EPIChashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq Rapid Exomehashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
Step Data
Group of Defaults = TruSeq Methyl Capture EPIC
Step 3: Clean Up Fragmented DNA (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Clean Up Fragmented DNA (TruSeq Methyl Capture EPIC v2.0)
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 4: Repair Ends (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Repair Ends (TruSeq Methyl Capture EPIC v2.0)
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 5: Adenylate 3' Ends (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Adenylate 3' Ends v2.0
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 6: Ligate Adapters (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Ligate Adapters v2.0
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Copy to Outputhashtag Trigger Location = Not Used
chevron-right Set Next Step and Copy to Inputhashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Label Groups
TruSeq Methyl Capture EPIC
Step Data (Master Step Fields)
Step 7: Clean Up and Pool Ligated Fragments (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Clean Up and Pool v2.0
Aliquot Generation = Fixed, 1
Naming Convention = {PoolName}
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Defaults
Sample Grouping = Group by Containers
Step File Placeholders
Log File - Manually uploaded
Step 8: Hybridize Probes (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Hybridize v2.0
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Library Dilutionhashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 9: Capture Hybridized Probes (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Capture Hybridized Probes v2.0
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Calculate Master Mixhashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 10: Perform Second Hybridization (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Perform Second Hybridization v2.0
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 11: Perform Second Capture (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Perform Second Capture (TruSeq Methyl Capture EPIC v2.0)
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - IF/ELSEhashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 12: Bisulfite Conversion (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Bisulfite Conversion v1.0
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Copy Placement Informationhashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 13: Amplify Enriched Library (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Amplify Enriched Library v2.0
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 14: Clean Up Amplified Enriched Library (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Clean Up v2.0
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 15: Bioanalyzer QC (Library Validation) (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Bioanalyzer QC (Library Validation) v2.0
Measurement Generation = Fixed, 1
chevron-right Generate Bioanalyzer Input filehashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Parse Bioanalyzer XML, Calculate nM and assign QC flagshashtag Trigger Location = Record Details
Trigger Style = Manual button
chevron-right Set Next Step - Output PASS/FAILhashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Parse Bioanalyzer XML and assign QC flagshashtag Trigger Location = Not Used
chevron-right Parse Bioanalyzer XML, Assign QC flags, and Copy Concentrationshashtag Trigger Location = Not Used
chevron-right Parse Bioanalyzer XML, Copy nM and Assign QC flagshashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Group of Defaults
chevron-right Nextera DNA Flex Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right Nextera Mate Pair Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right Nextera XT DNA Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right NRCC Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq ChIP-Seq Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TruSeq Exome Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq Methyl Capture EPIC Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq Rapid Exome Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq RNA Access Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq RNA Exome Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSeq Small RNA Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TruSeq Stranded mRNA Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TruSeq Stranded Total RNA Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TruSeq Targeted RNA Expression Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Peak 2 Size - bp
chevron-right TruSight Myeloid Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TruSight RNA Fusion Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
chevron-right TSCA Library Validationhashtag Criteria 1 - Operator = >=
Criteria 1 - Source Data Field = Region 1 Average Size - bp
Step Data
Group of Defaults = TruSeq Methyl Capture EPIC Library Validation
Step 16: Normalize Libraries (TruSeq Methyl Capture EPIC v2.0)
Master Step Name = Normalize Libraries 1 v2.0.10
Derived Sample Generation = Fixed, 1
chevron-right Normalization Calculations - Option 1hashtag Trigger Location = Record Details
Trigger Style = Manual button
chevron-right Set Next Step - Removehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right Routing script - Normalize Librarieshashtag Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Control Types
HCC1187 breast cancer tumor (TruSeq Methyl EPIC v1.0)
HCC1187 normal (BL) (TruSeq Methyl EPIC v1.0)
Catalog Number = CRL2322-D
Jurkat (TruSeq Methyl EPIC v1.0)
Catalog Number = D1255815
NA12878 (TruSeq Methyl EPIC v1.0)
Supplier = Coriell Institute
Sample Table
Column Headers
Additional Options and Dropdown Items
Placement Pattern = Row
Decimal Places Displayed = 2
Presets
HCC1187 normal (BL) (ATCC, catalog # CRL2323-D)
NA12878 (Coriell Institute, catalog # NA12878)
HCC1187 breast cancer tumor (ATCC, catalog # CRL2322)
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Step File Placeholders
Log File - Manually uploaded
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {InputItemName} Bioanalyzer
Sample Table
Column Headers
Additional Options and Dropdown Items
Placement Pattern = Column
Destination Containers
BioAnalyzer DNA 1000 Chip
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Use strict matching for Bioanalyzer results = No
Criteria 1 - Threshold Value = 100.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Use strict matching for Bioanalyzer results = No
Criteria 1 - Threshold Value = 5.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Molarity
Criteria 2 - Threshold Value = 10.00
Use strict matching for Bioanalyzer results = No
Criteria 1 - Threshold Value = 100.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 300.00
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Use strict matching for Bioanalyzer results = No
Additional Options and Dropdown Items
Criteria 1 - Source Data Field
Criteria 1 - Threshold Value
Decimal Places Displayed = 2
Criteria 2 - Source Data Field
Criteria 2 - Threshold Value
Decimal Places Displayed = 2
Use strict matching for Bioanalyzer results
Step File Placeholders
Bioanalyzer Driver File - Automatically attached
Bioanalyzer Driver File Generation Log File - Automatically attached
Bioanalyzer XML Result File (required) - Manually uploaded
Result File (optional) - Manually uploaded
PDF Summary File (optional) - Manually uploaded
Bioanalyzer XML Parsing Log File - Automatically attached
QC Assignment Log File - Automatically attached
QC Assignment Report - Automatically attached
Sample Table
Sample Display Default = Expand
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {SubmittedSampleName}
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Table Columns - Global Fields
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Well Sort Order = Row
Sample Display Default = Expand
Table Columns - Global Fields
Additional Options and Dropdown Items
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 5 - Probes
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Placement Pattern = Row
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 5 - Probes
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
ℹ The actual TruSeq Methyl Capture EPIC version used in the automation script may vary depending on the version of IPP installed.
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Manually uploaded
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 1 - Bisulphite Conversion Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
KAPA HiFi HotStart Uracil+ Ready Mix (2X)
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Reagent Kits
TruSeq Methyl Capture EPIC HT/LT - Box 2 - SPB Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
TruSeq Methyl Capture EPIC HT/LT - Box 4 - Core Reagents
Catalog Number = LT Catalog # - FC-151-1002; HT Catalog # - FC-151-1003
Sample Table
Column Headers
Additional Options and Dropdown Items
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
Naming Convention = {InputItemName} Bioanalyzer
Sample Table (Column Headers)
Additional Options and Dropdown Items
Placement Pattern = Column
Destination Containers
BioAnalyzer DNA High Sensitivity Chip
BioAnalyzer DNA 1000 Chip
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,500.00
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Average Size - bp
Criteria 2 - Threshold Value = 400.00
Criteria 1 - Threshold Value = 250.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Criteria 1 - Threshold Value = 350.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Average Size - bp
Criteria 2 - Threshold Value = 400.00
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 1,000.00
Criteria 1 - Threshold Value = 200.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 300.00
Criteria 1 - Threshold Value = 200.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 500.00
Criteria 1 - Threshold Value = 200.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 500.00
Criteria 1 - Threshold Value = 200.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 320.00
Criteria 1 - Threshold Value = 100.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Average Size - bp
Criteria 2 - Threshold Value = 200.00
Criteria 1 - Threshold Value = 250.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Average Size - bp
Criteria 2 - Threshold Value = 275.00
Criteria 1 - Threshold Value = 250.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Average Size - bp
Criteria 2 - Threshold Value = 275.00
Criteria 1 - Threshold Value = 100.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Peak 2 Size - bp
Criteria 2 - Threshold Value = 300.00
Criteria 1 - Threshold Value = 150.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Size - bp
Criteria 2 - Threshold Value = 400.00
Criteria 1 - Threshold Value = 160.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Size - bp
Criteria 2 - Threshold Value = 700.00
Criteria 1 - Threshold Value = 300.00
Criteria 2 - Operator = <=
Criteria 2 - Source Data Field = Region 1 Size - bp
Criteria 2 - Threshold Value = 400.00
Additional Options and Dropdown Items
Criteria 1 - Source Data Field
Criteria 1 - Threshold Value
Decimal Places Displayed = 2
Criteria 2 - Source Data Field
Criteria 2 - Threshold Value
Decimal Places Displayed = 2
Use strict matching for Bioanalyzer results
Step File Placeholders
Bioanalyzer Input File - Automatically attached
Bioanalyzer Input File Generation Log File - Automatically attached
Bioanalyzer XML Result File (required) - Manually uploaded
Result File (optional) - Manually uploaded
PDF Summary File (optional) - Manually uploaded
Bioanalyzer XML Parsing Log File - Automatically attached
QC Assignment Log File - Automatically attached
QC Assignment Report - Automatically attached
Sample Table
Sample Display Default = Expand
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Naming Convention = {InputItemName}
ℹ The field value and the actual version of the workflows and steps in the routing automation script may be different depending on the version of IPP installed.
Sample Table
Column Headers
Additional Options and Dropdown Items
Decimal Places Displayed = 2
Target Normalization (nM)
Decimal Places Displayed = 2
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Table Columns - Global Fields
ℹ The preset options for Derived Sample Sequencing Instrument may vary depending on the version of the IPP.
Additional Options and Dropdown Items
Control Concentration (ng/ul)
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Additional Options and Dropdown Items
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp '(step.::Total Samples:: = step.::Total Samples:: + 1)' -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'step.::RSB (mL):: = step.::Total Samples:: * 5 * 1.1 ; step.::0.5 M EDTA (uL):: = step.::Total Samples:: * 10 * 1.1 ; if (!input.name.contains(::TruSeq Methyl EPIC v1.0::)) {output.::Concentration:: = input.::Concentration:: ; output.::Conc. Units:: = input.::Conc. Units::}' -log {compoundOutputFileLuid1}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'if (input.name.contains( ::(TruSeq Methyl EPIC v1.0)::) ) { output.::Concentration:: = step.::Control Concentration (ng/ul):: ; output.::Conc. Units:: = ::ng/uL:: ; output.::Sample Volume (ul):: = 500 / step.::Control Concentration (ng/ul):: ; output.::Buffer Volume (ul):: = 50 - output.::Sample Volume (ul):: } else {output.::Sample Volume (ul):: = 500 / input.::Concentration:: ; output.::Buffer Volume (ul):: = 50 - output.::Sample Volume (ul)::}' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
script:driver_file_generator \
-i {processURI:v2} \
-t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv \
-o {compoundOutputFileLuid0}.csv \
-l {compoundOutputFileLuid1} \
&& /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
script:addBlankLines \
-i {stepURI:v2} \
-f {compoundOutputFileLuid0}.csv \
-l {compoundOutputFileLuid1} \
-sep COMMA \
-b ',False,' \
-h 1 \
-c LIMSID \
-pre 'Sample '"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {processURI:v2} \
script:parseBioAnalyzer \
-inputFile {compoundOutputFileLuid2} \
-log {compoundOutputFileLuid5} \
-configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' \
script:assignQC \
-log {compoundOutputFileLuid6} \
-qcResult {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t true \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -excludeControls true -exp 'if (output.QC == true) { nextStep = ::ADVANCE:: } else { nextStep = ::ESCALATE:: }' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'nextStep = ::ADVANCE:: ; input.::Concentration (ng/ul):: = output.::Concentration::' -log {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'output.::Target Insert Size (bp):: = input.::Target Insert Size (bp)::' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'nextStep = ::ADVANCE:: ; input.::Concentration (ng/ul):: = output.::Concentration::' -log {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2:http} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Library Volume (ul):: = 200 / input.::Concentration (ng/ul):: ; output.::RSB Volume (ul):: = 10 - output.::Library Volume (ul)::' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2:http} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'step.::Total Number of Samples:: = step.::Total Number of Samples:: + 1' -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2:http} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'step.::Enrichment Elution Buffer 1 (ul):: = 28.5 * step.::Total Number of Samples:: ; step.::2N NaOH (ul):: = 1.5 * step.::Total Number of Samples::' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'if (step.::Bisulfite Conversion?:: == ::True:: ) {nextStep = ::Bisulfite Conversion (TruSeq Methyl Capture EPIC v2.0.11):: } else {nextStep = ::Amplify Enriched Library (TruSeq Methyl Capture EPIC v2.0.11):: }' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'output.::Sample_Plate:: = output.container.name' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
script:evaluateDynamicExpression \
-t false \
-h false \
-exp 'nextStep = ::ADVANCE::' \
-log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar script:driver_file_generator -i {processURI:v2} -u {username} -p {password} -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv -o {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar script:addBlankLines -i {stepURI:v2} -u {username} -p {password} -f {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1} -sep COMMA -b ',False,' -h 1 -c LIMSID -pre 'Sample '"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; output.::Molarity (nM):: = (output.::Concentration:: * 1000000) / (660 * output.::Region 1 Average Size - bp::) ; input.::Molarity (nM):: = output.::Molarity (nM):: ; output.::Conc. Units:: = ::ng/ul::' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -excludeControls true -exp 'if (output.QC == true) { nextStep = ::ADVANCE:: } else { nextStep = ::ESCALATE:: }' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; input.::Concentration:: = output.::Concentration:: ; output.::Conc. Units:: = ::ng/ul:: ; input.::Conc. Units:: = output.::Conc. Units::' -log {compoundOutputFileLuid8}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'if (output.::Conc. Units::.contains(::pg::)) {output.::Molarity (nM):: = output.::Region 1 Molarity:: / 1000} else {output.::Molarity (nM):: = output.::Region 1 Molarity::} ; (input.::Molarity (nM):: = output.::Molarity (nM)::) ' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Molarity (nM):: = input.::Molarity (nM):: ; if (output.::Molarity (nM):: <= step.::Target Normalization (nM)::) {output.::Sample Volume (ul):: = step.::Final Volume (ul):: ; output.::Buffer Volume (ul):: = 0 ; output.::Normalized Molarity (nM):: = output.::Molarity (nM)::} else {output.::Sample Volume (ul):: = (step.::Target Normalization (nM):: * step.::Final Volume (ul):: ) / input.::Molarity (nM):: ; output.::Buffer Volume (ul):: = step.::Final Volume (ul):: - output.::Sample Volume (ul):: ; output.::Normalized Molarity (nM):: = step.::Target Normalization (nM)::}' -log {compoundOutputFileLuid0}"
Copy bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'nextStep = ::REMOVE::' -log {compoundOutputFileLuid0}"
Copy bash -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {stepURI:v2} -l {compoundOutputFileLuid0} script:changeWorkflow \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'MiSeq' \
--WORKFLOW 'MiSeq Sequencing v3.2' \
--STEP 'Library Pooling (MiSeq v3.2)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NextSeq' \
--WORKFLOW 'NextSeq 500/550 Sequencing v1.2' \
--STEP 'Library Pooling (NextSeq 500/550 v1.2)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NovaSeq 2.0' \
--WORKFLOW 'NovaSeq 6000 v2.3' \
--STEP 'Define Run Format (NovaSeq 6000 v2.3)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NovaSeq 3.0' \
--WORKFLOW 'NovaSeq 6000 v3.8' \
--STEP 'Define Run Format (NovaSeq 6000 v3.8)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NovaSeqDx' \
--WORKFLOW 'NovaSeqDx v1.2' \
--STEP 'Define Run Format (NovaSeqDx v1.2)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NextSeq 1000/2000' \
--WORKFLOW 'NextSeq 1000/2000 Sequencing v2.4' \
--STEP 'Library Pooling and Dilution (NextSeq 1000/2000 Sequencing v2.4)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NovaSeq X Series' \
--WORKFLOW 'NovaSeq X Series v1.1' \
--STEP 'Assign Analysis Configuration Template (NovaSeq X Series Sequencing v1.1)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS' \
\
--FIELD_NAME 'Sequencing Instrument' \
--FIELD_VALUE 'NextSeq 1000/2000 On-Prem' \
--WORKFLOW 'NextSeq 1000/2000 On-Prem Sequencing v1.0' \
--STEP 'Library Pooling and Dilution (NextSeq 1000/2000 On-Prem Sequencing v1.0)' \
--INPUTS_OR_OUTPUTS 'OUTPUTS'" Conc. = 20
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 1 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 2 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 3 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 4 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 5 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 1 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 2 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 3 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 4 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Region 5 Average Size - bp
Decimal Places Displayed = 0
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
Decimal Places Displayed = 2
HeLa (Biochain, catalog # D1255811)
Jurkat (Biochain, catalog # D1255815)
Region 1 Average Size - bp
Region 2 Average Size - bp
Region 3 Average Size - bp
Region 4 Average Size - bp
Region 5 Average Size - bp
Region 1 Average Size - bp
Region 2 Average Size - bp
Region 3 Average Size - bp
Region 4 Average Size - bp
Region 5 Average Size - bp
Region 1 Average Size - bp
Region 2 Average Size - bp
Region 3 Average Size - bp
Region 4 Average Size - bp
Region 5 Average Size - bp
Region 1 Average Size - bp
Region 2 Average Size - bp
Region 3 Average Size - bp
Region 4 Average Size - bp
Region 5 Average Size - bp
Additional Options and Dropdown Items
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Decimal Places Displayed = 2
Additional Options and Dropdown Items
Additional Options and Dropdown Items
NextSeq 1000/2000 On-Prem