NovaSeq X Series On-Prem v1.1.0

Release Date: April 2026

These Release Notes describe the key changes to software components for the Clarity LIMS NovaSeq X Series On Premise Integration Package version 1.1.0.

Compatibility

Refer to Compatibility under Instruments & Integrations.

New Features

Features provided with this release of the integration package are as follows.

• Create planned runs without analysis (Generate BCLs only)
• Sequencing run metrics are now displayed per-run and per-lane in the AUTOMATED - Sequencing Run step
• Added support for NovaSeq X 5B flow cell types
• Added support for NovaSeq X 1.5B flow cell with 600 cycle kit
• New NovaSeq X Series On-Prem Sequencing v2.0 workflow available on Illumina Preset Protocols v2.12.
• The Make Bulk Pool and Calculate Volumes automation scripts have been updated to align with the latest NovaSeq X Series System Sequencing Protocol.

Enhancement

• The integration supports --bcl-sampleproject-subdirectories option of BCL Convert.
• Both indexed and pooled libraries are now supported as input for the NovaSeq X v2.0 sequencing protocol.

Defects Fixed

• Fixed Calculation automation on Dilute and Denature step

Known Limitations

• Tracks only the analysis configured with the planned run. Does not track analysis requeue or analysis triggered externally with the same planned run.
• The integration does not support pools with samples assigned with Analysis Configuration Templates from different instruments.
• BCL autodetect is not supported
• The override cycles will shows 151 cycles instead of 301 cycles for Read 1 and Read 2 when choosing TruSeq PCR-Free kit and TruSeq Nano Library Prep Kit with 1.5B flowcell with 600 cycle kit. Refer to NovaSeq X Series On-Prem Integration v1.1.0 User Interaction, Validation and Troubleshooting for troubleshooting guide.