# NovaSeq X Series v1.4.0

Release Date: April 2026

These release notes describe the key changes to software components for the Clarity LIMS NovaSeq X Series Integration Package v1.4.0.

## Prerequisites

* BaseSpace Sequence Hub account that has the Enterprise or Professional subscription

## Compatibility

Refer to [Compatibility](/clarity-lims/instruments-and-integrations/compatibility.md#novaseq-x-series) under Instruments & Integrations.

## New Features

Features provided with this release of the integration package are as follows.

* Create planned runs without analysis (Generate BCLs only)
* Sequencing run metrics are now displayed per-run and per-lane in the AUTOMATED - Sequencing Run step
* Added support for NovaSeq X 5B flow cell type
* Added support for NovaSeq X 1.5B flow cell with 600 cycle kit
* The Make Bulk Pool and Calculate Volumes automation scripts have been updated to align with the latest NovaSeq X Series System Sequencing Protocol.
* New NovaSeq X Series Sequencing v2.0 workflow available on Illumina Preset Protocols v2.12.

{% hint style="info" %}
Refer to [NovaSeq X Series Sequencing v2.0 Configuration](/clarity-lims/instruments-and-integrations/novaseq-x-series/novaseq-x-series-workflow-and-configuration/novaseq-x-series-workflow-and-configuration-v2.0/novaseq-x-series-sequencing-v2.0-configuration.md) for details on changes in automations, configuration and run metrics.
{% endhint %}

## Enhancement

* Both indexed and pooled libraries are now supported as input for the NovaSeq X Series Sequencing v2.0 protocol.
* The integration support *--bcl-sampleproject-subdirectories* option of BCL Convert.
* Running of 'Validate Run Setup and Create Sample Sheet' more than one time now update the created planned run instead of creating as duplicate planned run
* Ability to create Sample Sheet without creating the planned run.

## Defects Fixed

* Fixed Calculation automation on Dilute and Denature step

## Known Limitations

* Tracks only the analysis configured with the planned run. Does not track analysis requeue or analysis triggered externally with the same planned run.
* The integration does not support pools with samples assigned with Analysis Configuration Templates from different instruments.
* The cloud integration does not support local analysis. Error message is displayed when Create Planned Run is selected.
* The override cycles will shows 151 cycles instead of 301 cycles for Read 1 and Read 2 when choosing TruSeq PCR-Free kit and TruSeq Nano Library Prep Kit with 1.5B flowcell with 600 cycle kit. Refer to [NovaSeq X Series Integration v1.4.0 User Interaction, Validation and Troubleshooting](/clarity-lims/instruments-and-integrations/novaseq-x-series/novaseqx/novaseqx-v1.4.0-release-notes/novaseqx-v1.4.0-user-interaction-validation-and-troubleshooting.md#values-in-override-cycles-is-different-from-read-lengths-in-the-run-review-page) for troubleshooting guide.
* Retrieve ACT List automation will fail when ACT contain obsolete kits. Refer to [NovaSeq X Series Integration v1.4.0 User Interaction, Validation and Troubleshooting](/clarity-lims/instruments-and-integrations/novaseq-x-series/novaseqx/novaseqx-v1.4.0-release-notes/novaseqx-v1.4.0-user-interaction-validation-and-troubleshooting.md#retrieve-act-list-fails-when-assigning-analysis-configuration-template) for troubleshooting guide.


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