NovaSeq X Series v1.4.0

Release Date: April 2026

These release notes describe the key changes to software components for the Clarity LIMS NovaSeq X Series Integration Package v1.4.0.

Prerequisites

• BaseSpace Sequence Hub account that has the Enterprise or Professional subscription

Compatibility

Refer to Compatibility under Instruments & Integrations.

New Features

Features provided with this release of the integration package are as follows.

• Create planned runs without analysis (Generate BCLs only)

• Sequencing run metrics are now displayed per-run and per-lane in the AUTOMATED - Sequencing Run step

• Added support for NovaSeq X 5B flow cell type

• Added support for NovaSeq X 1.5B flow cell with 600 cycle kit

• The Make Bulk Pool and Calculate Volumes automation scripts have been updated to align with the latest NovaSeq X Series System Sequencing Protocol.

• New NovaSeq X Series Sequencing v2.0 workflow available on Illumina Preset Protocols v2.12.

Refer to NovaSeq X Series Sequencing v2.0 Configuration for details on changes in automations, configuration and run metrics.

Enhancement

• Both indexed and pooled libraries are now supported as input for the NovaSeq X Series Sequencing v2.0 protocol.

• The integration support --bcl-sampleproject-subdirectories option of BCL Convert.

• Running of 'Validate Run Setup and Create Sample Sheet' more than one time now update the created planned run instead of creating as duplicate planned run

• Ability to create Sample Sheet without creating the planned run.

Defects Fixed

• Fixed Calculation automation on Dilute and Denature step

Known Limitations

• Tracks only the analysis configured with the planned run. Does not track analysis requeue or analysis triggered externally with the same planned run.

• The integration does not support pools with samples assigned with Analysis Configuration Templates from different instruments.

• The cloud integration does not support local analysis. Error message is displayed when Create Planned Run is selected.

• The override cycles will shows 151 cycles instead of 301 cycles for Read 1 and Read 2 when choosing TruSeq PCR-Free kit and TruSeq Nano Library Prep Kit with 1.5B flowcell with 600 cycle kit. Refer to NovaSeq X Series Integration v1.4.0 User Interaction, Validation and Troubleshooting for troubleshooting guide.

• Retrieve ACT List automation will fail when ACT contain obsolete kits. Refer to NovaSeq X Series Integration v1.4.0 User Interaction, Validation and Troubleshooting for troubleshooting guide.