# IPP v2.4.0

Release Date: April 2023

These Release Notes describe the key changes to the Illumina Preset Protocols (IPP) from version 2.3.

This release is an optional software update for customers interested in the new features available.

If upgrading from a version before version 2.2, review the release notes for v2.2 for a list of features and bug fixes introduced in that version.

## Compatibility

* Clarity LIMS v5.1 and later
* NGS Extensions Package v5.22 and later

## New Features

* Supports the following workflows:
  * NovaSeqDx v1.0
  * IGA NovaSeq Sequencing v2.0
  * IGA Library Prep Automated v2.0
  * NextSeq 1000/2000 v2.3 workflow (has the P1/P2 long read reagent cartridge support)
  * Library Prep Validation v2.2.0 workflow (routes to the NovaSeqDx instrument)

## Defects Fixed

* Fixed dilution calculation for Illumina DNA Prep with Enrichment S Tagmentation workflow as follows.
  * Renamed step custom fields.
  * Modified the Sample Details table.
  * Modified the Calculate Dilution Volume & Total Volume and Set Next Step - Remove & Copy NFE Sample Type and Desired gDNA Input (ng) automation scripts in the Dilute gDNA and Dilute FFPE DNA steps of the Prep Sample Protocol.
* Removed the unsupported None option for the Analysis Workflow field in the Load to Reagent Cartridge step of the NextSeq 1000/2000 workflow. All planned runs that are created have the analysis configuration associated.
* Fixed the duplicated Normalized Libraries 1 master step for the Nextera Mate Pair and TruSeq Methyl Capture EPIC workflows.
* Fixed the concentration limit in the Illumina DNA PCR-Free Library Prep Manual workflow.
* Made the Samplesheet Format field a required field for the NovaSeq 6000 workflow.
* Fixed the Validate Library Tube automation script to accept only an uppercase barcode in the NovaSeq 6000 workflow.

## Known Issues

This release contains the following known issues in the NextSeq 1000/2000 v2.1 workflow:

* Sample Name can only contain alphanumeric, dash, and underscore characters. For more information, refer to NextSeq 1000/2000 Integration v2.3 Validation and Troubleshooting in the Clarity LIMS Integrations & Tool Kits documentation.
* Having two samples with the same name in the same sequencing run is not supported.