Master Step Name = Extend and Stain BeadChip-Infinium v1.2
Step Type = No Outputs
Reagent Kits
Automations
Set Array Instrument
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Routing Script - iScan
Trigger Location = Step
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Naming Convention = {InputItemName}
Reagent Kits
MA1-Infinium
Supplier = Illumina
Website =
MA2-Infinium
Supplier = Illumina
Website =
NaOH
RAM-Infinium
Supplier = Illumina
Website =
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Placement Pattern = Column
Destination Containers
96 well plate
Default = Infinium XT iSelect-96 Kit
Instruction Notes
Multiline Text
Read Only
Default =
Add 20 µl MA1 to each well of the plate.
Transfer 4 µl DNA sample from the DNA plate to the corresponding wells of the plate.
Add 4 µl 0.1N NaOH in to each sample well of the plate.
Add 35 μl MA2 in to each sample well of the plate.
Add 35 μl RAM in to each sample well of the plate.
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Default = Incubate the plate in the Illumina Hybridization Oven for 20–24 hours at 37°C.
ℹ The workflow version and step version for the Array Instrument may vary depending on the version of the IPP.
Sample Table (Column Headers)
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Derived Sample
DNA Plate Barcode
Text
Read Only
Derived Sample
Infinium Kit Name
Text
Read Only
Default =
Without allowing pipette tips to touch BeadChip surfaces, fill the reservoir of each flow-through chamber as follows:
150 µl RA1. Incubate for 30 seconds. Repeat 5 times.
225 µl LX1. Incubate for 10 minutes.Repeat once.
225 µl LX2. Incubate for 10 minutes. Repeat once.
300 µl EML. Incubate for 15 minutes.
250 µl 95% formamide/1 mM EDTA. Incubate for 1 minute. Repeat twice.Incubate 5 minutes.
Set the the chamber rack temperature to the temperature indicated on the SML tube.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Stain Instruction Notes
Multiline Text
Read Only
Default =
Fill the reservoir of each flow-through chamber as follows:
250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl ATM. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes. g 250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl ATM. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
250 µl SML. Incubate for 10 minutes.
250 µl XC3. Incubate for 1 minute. Repeat twice.
Wait 5 minutes.
Wash and Coat Beadships Instruction Notes
Multiline Text
Read Only
Default =
Add 310 ml PB1 to the PB1 wash dish.
Add 310 ml XC4 to the XC4 wash dish
