The Library Prep AmpliSeq for Illumina Cancer HotSpot Panel V2 is part of the AmpliSeq for Illumina Cancer HotSpot Panel V2 protocols that include the following functionality:
Preconfigured AmpliSeq for Illumina Cancer HotSpot Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol 1: Library Prep (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Dilute DNA (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Dilute DNA (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Automations
Copy Concentration and Set Sample Volume
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Calculate Input Amount
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 2: Qubit (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Qubit (AmpliSeq for Illumina v1.1)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Automations
Assign QC flags (Qubit QC)
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step and Copy Concentration
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 3: Dilute DNA to Final Concentration (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Dilute DNA (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Automations
Copy Concentration and Set Sample Volume
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Calculate Input Amount
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 4: Amplify Targets (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Amplify Targets (AmpliSeq for Cancer Hotspot Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Automations
Concentration,Total Volume and Input Amount - Cancer HotSpot v2
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Calculate Prep Input - CH v2
Trigger Location = Record Details
Trigger Style = Manual button
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 5: Partially Digested Amplicons (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Partially Digested Amplicons (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 6: Ligate Indexes (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Ligate Indexes (AmpliSeq for Illumina v1.1)
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Route AmpliSeq Samples - CHP v2
Trigger Location = Step
Trigger Style = Automatic upon exit
Route AmpliSeq Samples - Immune Repertoire Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - BRCA
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - Childhood Cancer Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - CP
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - Custom DNA Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - Focus Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - Immune Response Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples - Myeloid Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
Route AmpliSeq Samples -Transcriptome Human GEx Panel
Trigger Location = Not Used
ℹ The workflow version and step version for the routing script may vary depending on the version of the IPP.
The Equalizer Workflow AmpliSeq for Illumina Cancer HotSpot Panel V2 is part of the AmpliSeq for Illumina Cancer HotSpot Panel V2 protocols that include the following functionality:
Preconfigured AmpliSeq for Illumina Cancer HotSpot Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol 1: Equalizer Workflow (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Automations
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Calculate Total Samples and Calculate Master Mix
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Group of Defaults
AmpliSeq for CHS V2
Thermal Cycler Program = AMP
Equalizer Workflow
Thermal Cycler Program = EQUAL
Standard Workflow
Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Equalizer Workflow
Master Step Fields
Step 3: Perform Capture and Clean Up (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Perform Capture and Clean Up (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step File Placeholders
Log - Automatically attached
Sample Table
Step 4: Elute Library (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Elute Library (AmpliSeq for Illumina v1.1)
The Standard Workflow AmpliSeq for Illumina Cancer HotSpot Panel V2 is part of the AmpliSeq for Illumina Cancer HotSpot Panel V2 protocols that include the following functionality:
Preconfigured AmpliSeq for Illumina Cancer HotSpot Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol 1: Standard Workflow (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Protocol Type = Sample Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Calculate Total Samples and Calculate Master Mix
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Group of Defaults
AmpliSeq for CHS V2
Thermal Cycler Program = AMP
Equalizer Workflow
Thermal Cycler Program = EQUAL
Standard Workflow
Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Standard Workflow
Master Step Fields
Step 3: Perform Second Clean Up (AmpliSeq for Illumina Cancer HotSpot Panel V2 v1.1)
Master Step Name = Perform Second Clean Up (AmpliSeq for Illumina v1.1)
