TruSight Oncology 500 ctDNA v1.1 TruSight Oncology 500 ctDNA v1.1 includes the following functionality:
Preconfigured TruSight Oncology 500 ctDNA v1.1 protocol that supports the preparation of 48 libraries from cfDNA.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples (when necessary).
Automated assignment of QC Pass/Fail, based on user-selected threshold values. A routing script that allows sequencing of libraries using any Illumina sequencing instrument.
Protocol 1: TruSight Oncology 500 ctDNA v1.1
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Prepare cfDNA, Perform End Repair and A-Tailing (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Prepare cfDNA, Perform End Repair and A-Tailing (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Count Samples and Calculate Master Mixhashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Calculate Normalizationhashtag Trigger Location = Record Details
Trigger Style = Manual button
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Ligate Adapters (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Ligate Adapters (TruSight Oncology 500 ctDNA v1.1)
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 3: Clean Up Ligation (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Clean Up Ligation (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 4: Index PCR (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Index PCR (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Label Groups
TruSight Oncology 500 ctDNA NovaSeq
Step Data (Master Step Fields)
Step 5: Set Up First Hybridization (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Set Up First Hybridization (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 6: Capture Target One (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Capture Targets (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Count Samples and Calculate Master Mixhashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 7: Set Up Second Hybridization (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Set Up Second Hybridization (TruSight Oncology 500 ctDNA v1.1)
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 8: Capture Target Two (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Capture Targets (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Count Samples and Calculate Master Mixhashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 9: Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 10: Clean Up Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)
Master Step Name = Clean Up Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)
Derived Sample Generation = Fixed, 1
chevron-right Set Next Step - Advancehashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)