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TruSight Oncology 500 ctDNA v1.1

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Overview

TruSight Oncology 500 ctDNA v1.1 includes the following functionality:

  • Preconfigured TruSight Oncology 500 ctDNA v1.1 protocol that supports the preparation of 48 libraries from cfDNA.

  • Automated calculation of sample and buffer volumes.

  • Automated calculation or display of reagents at every step in the protocol.

  • Automatic step transition when required.

  • Automatic placement of samples (when necessary).

  • Automated assignment of QC Pass/Fail, based on user-selected threshold values. A routing script that allows sequencing of libraries using any Illumina sequencing instrument.

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Protocol 1: TruSight Oncology 500 ctDNA v1.1

Protocol Type = Library Prep

Next Steps Configuration

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Step 1: Prepare cfDNA, Perform End Repair and A-Tailing (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Prepare cfDNA, Perform End Repair and A-Tailing (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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The version of Prepare cfDNA, Perform End Repair and A-Tailing master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightCount Samples and Calculate Master Mixhashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightCalculate Normalizationhashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Placement = Enabled

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 2: Ligate Adapters (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Ligate Adapters (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = No Outputs

  • Reagent Kits

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The version of Ligate Adapters master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 3: Clean Up Ligation (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Clean Up Ligation (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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The version of Clean Up Ligation master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 4: Index PCR (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Index PCR (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Add Labels

  • Derived Sample Generation = Fixed, 1

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The version of Index PCR master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Add Labels

  • Label Groups

    • TruSight Oncology 500 ctDNA NovaSeq

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Record Details

  • Step Data (Master Step Fields)

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Step 5: Set Up First Hybridization (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Set Up First Hybridization (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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The version of Set Up First Hybridization master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 6: Capture Target One (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Capture Targets (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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The version of Capture Targets master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightCount Samples and Calculate Master Mixhashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 7: Set Up Second Hybridization (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Set Up Second Hybridization (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = No Outputs

  • Reagent Kits

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The version of Set Up Second Hybridization master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 8: Capture Target Two (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Capture Targets (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

circle-info

The version of Capture Targets master step name may be different depending on the version of IPP installed.

hashtag
Automations

chevron-rightCount Samples and Calculate Master Mixhashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 9: Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = No Outputs

  • Reagent Kits

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The version of Amplified Enriched Libraries master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 10: Clean Up Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)

  • Master Step Name = Clean Up Amplified Enriched Libraries (TruSight Oncology 500 ctDNA v1.1)

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

circle-info

The version of Clean Up Amplified Enriched Libraries master step name may be different depending on the version of IPP installed.

hashtag
Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

hashtag
Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 2

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

    • TruSight Oncology 500 ctDNA, Box 3

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Placement Pattern = Column
  • Destination Containers

    • 96 well plate

  • Decimal Places Displayed = 2

    ERA1-B (uL)

    Numeric

    Decimal Places Displayed = 2

    Final Volume (uL)

    Numeric

    Default = 50

    Total Samples

    Numeric

    Default = 0

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • TruSight Oncology 500 ctDNA, Box 1
    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website = www.illumina.comarrow-up-right

  • TruSight Oncology 500 ctDNA, Box 2

    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website =

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Default = 5

    Add UMI1 (uL) to each well

    Numeric

    Default = 10

    Note

    Multiline Text

    Default = Immediately after ligation: add 5ul STL

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 3

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Step File Placeholders
    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
    Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Default = 5

    Thermal Cycler Program

    Text

    Default = I-PCR

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 5

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

    • TruSight Oncology 500 ctDNA, Box 6

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

    • TruSight Oncology 500 ctDNA, Box 7

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Default = 10

    Add TCB1 (uL) to each well

    Numeric

    Default = 15

    Thermal Cycler Program

    Text

    Default = HYB1

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 5

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

    • TruSight Oncology 500 ctDNA, Box 6

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Decimal Places Displayed = 0

    Total Samples

    Numeric

    Default = 0

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • TruSight Oncology 500 ctDNA, Box 5
    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website = www.illumina.comarrow-up-right

  • TruSight Oncology 500 ctDNA, Box 6

    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website =

  • TruSight Oncology 500 ctDNA, Box 7

    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website =

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Default = 10

    Add TCB1 (uL) to each well

    Numeric

    Default = 15

    Thermal Cycler Program

    Text

    Default = HYB2

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 3

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

    • TruSight Oncology 500 ctDNA, Box 5

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

    • TruSight Oncology 500 ctDNA, Box 6

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Decimal Places Displayed = 2

    Total Samples

    Numeric

    Default = 0

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • TruSight Oncology 500 ctDNA, Box 6
    • Supplier = Illumina

    • Catalog Number = 20039252; 20039253; 20039254

    • Website = www.illumina.comarrow-up-right

    Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Default = 5

    Thermal Cycler Program

    Text

    Default = EL-PCR

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • TruSight Oncology 500 ctDNA, Box 3

      • Supplier = Illumina

      • Catalog Number = 20039252; 20039253; 20039254

      • Website =

  • Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Step File Placeholders
    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Desired Input (ng)

    Numeric

    Required Field

    Default = 30

    ERA1-A (uL)

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Add ALB1 (uL) to each well

    Numeric

    Default = 60

    Add LIG3 (uL) to each well

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    80% EtOH Prep Date

    Date

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Add EPM (uL) to each well

    Numeric

    Default = 20

    Add UPxx (uL) to each well

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Add OPD2 (uL) to each well

    Numeric

    Default = 5

    Add TCA1 (uL) to each well

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    EE2 (uL)

    Numeric

    Decimal Places Displayed = 0

    HP3 (uL)

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Add OPD2 (uL) to each well

    Numeric

    Default = 5

    Add TCA1 (uL) to each well

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    EE2 (uL)

    Numeric

    Decimal Places Displayed = 2

    HP3 (uL)

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Add EPM (uL) to each well

    Numeric

    Default = 20

    Add PPC3 (uL) to each well

    Numeric

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    80% EtOH Prep Date

    Date

    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'step.::Total Samples:: = step.::Total Samples:: + 1' \
    -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'output.::Concentration:: = input.::Concentration:: ; \
    output.::Conc. Units:: = input.::Conc. Units:: ; \
    step.::ERA1-B (uL):: = step.::Total Samples:: * (26/3) ; \
    step.::ERA1-A (uL):: = step.::Total Samples:: * (10/3) ' \
    -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t true \
    -h false \
    -exp 'output.::Sample Volume (uL):: = (step.::Desired Input (ng):: / output.::Concentration:: ) ; \
    output.::Buffer Volume (uL):: = step.::Final Volume (uL):: - output.::Sample Volume (uL):: ' \
    -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'step.::Total Samples:: = step.::Total Samples:: + 1' \
    -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'step.::EE2 (uL):: = step.::Total Samples:: * (95/3) ; \
    step.::HP3 (uL):: = step.::Total Samples:: * (5/3) ' \
    -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'step.::Total Samples:: = step.::Total Samples:: + 1' \
    -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
    script:evaluateDynamicExpression \
    -t false \
    -h false \
    -exp 'step.::EE2 (uL):: = step.::Total Samples:: * (95/3) ; \
    step.::HP3 (uL):: = step.::Total Samples:: * (5/3) ' \
    -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    Website = www.illumina.comarrow-up-right

    Conc. Units

    Text

    Required Field

    Derived Sample

    Sample Name

    Built-in

    Derived Sample

    Sample Volume (uL)

    Numeric

    Decimal Places Displayed = 2

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Well

    Built-in

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    Sample Name

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    Project Name

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    Website = www.illumina.comarrow-up-right
    Website = www.illumina.comarrow-up-right

    Well

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    Sample Name

    Built-in

    Project

    Project Name

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    Website = www.illumina.comarrow-up-right

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

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    Project

    Project Name

    Built-in

    Website = www.illumina.comarrow-up-right
    Website = www.illumina.comarrow-up-right

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

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    Project

    Project Name

    Built-in

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    Built-in

    Derived Sample

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    Options

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    Derived Sample

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    Required Field

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    Container

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    Built-in

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    Sample Name

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    Well

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    Derived Sample

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    Project Name

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    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right
    www.illumina.comarrow-up-right

    Derived Sample

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    Container

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    Container

    Container

    Container

    Container

    Container

    Container