The Standard Workflow AmpliSeq for Illumina BRCA Panel is part of the preconfigured AmpliSeq for Illumina BRCA Panel protocols supporting the preparation of uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
This protocol includes the following:
Preconfigured AmpliSeq for Illumina BRCA Panel protocol that supports the preparation of uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
A routing script that allows sequencing of libraries using any Illumina sequencing instrument.
Protocol 1: Standard Workflow (AmpliSeq for Illumina BRCA Panel v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina BRCA Panel v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina BRCA Panel v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
Automations
Count Samples and Calculate Master Mix
Trigger Location = Record Details
Trigger Style = Automatic upon entry
Set Next Step - Advance
Trigger Location = Record Details
Trigger Style = Automatic upon exit
Calculate Total Samples and Calculate Master Mix
Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Record Details
Group of Defaults
AmpliSeq for CHS V2
Thermal Cycler Program = AMP
Equalizer Workflow
Thermal Cycler Program = EQUAL
Standard Workflow
Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Standard Workflow
Master Step Fields
Step 3: Perform Second Clean Up (AmpliSeq for Illumina BRCA Panel v1.1)
Master Step Name = Perform Second Clean Up (AmpliSeq for Illumina v1.1)
