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Nextera Rapid Capture Custom Enrichment v2.0

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Protocol 1: Nextera Rapid Capture Custom Enrichment v2.0

Protocol Type = Library Prep

Next Steps Configuration

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Step 1: Tagment Genomic DNA (NRCC v2.0)

  • Master Step Name = Tagment v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

  • Naming Convention = {SubmittedSampleName}

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Automations

chevron-rightNormalize gDNAhashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightNormalize gDNA (Nextera XT)hashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Placement = Enabled

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 2: Clean Up Tagmented DNA (NRCC v2.0)

  • Master Step Name = Clean Up v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

  • Naming Convention = {InputItemName}

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 3: Bioanalyzer QC (DNA) (NRCC v2.0)

  • Master Step Name = Bioanalyzer QC (DNA) 5.1.2

  • Step Type = Standard QC

  • Measurement Generation = Fixed, 1

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The version of Bioanalyzer QC (DNA) master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightGenerate Bioanalyzer driver filehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightParse Bioanalyzer XML and assign QC flagshashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightSet Next Step - Output PASS/FAILhashtag
  • Trigger Location = Not Used

chevron-rightSet Next Step and Copy to Inputhashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Placement = Enabled

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

Group of Defaults

chevron-rightNRCChashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightPeak 2 Size Thresholdshashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq ChIP-Seqhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Molarity

chevron-rightTruSeq Methyl Capture EPIChashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq Rapid Exomehashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

  • Step Data

    • Group of Defaults = NRCC

    • Master Step Fields

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Step 4: Amplify Tagmented DNA (NRCC v2.0)

  • Master Step Name = Amplify Tagmented DNA v2.0

  • Step Type = Add Labels

  • Derived Sample Generation = Fixed, 1

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Add Labels

  • Label Groups

    • Nextera Rapid Capture Custom Enrichment (NRCC)

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Record Details

  • Step Data (Master Step Fields)

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Step 5: Clean Up Amplified DNA (NRCC v2.0)

  • Master Step Name = Clean Up v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

  • Naming Convention = {InputItemName}

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 6: Bioanalyzer QC (Library Validation) (NRCC v2.0)

  • Master Step Name = Bioanalyzer QC (Library Validation) v2.0

  • Step Type = Standard QC

  • Measurement Generation = Fixed, 1

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Automations

chevron-rightGenerate Bioanalyzer Input filehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightParse Bioanalyzer XML, Calculate nM and assign QC flagshashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Output PASS/FAILhashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightParse Bioanalyzer XML, Assign QC flags, and Copy Concentrationshashtag
  • Trigger Location = Not Used

chevron-rightParse Bioanalyzer XML and assign QC flagshashtag
  • Trigger Location = Not Used

chevron-rightParse Bioanalyzer XML, Copy nM and Assign QC flagshashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Placement = Enabled

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Column

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Record Details

  • Step Data (Master Step Fields)

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Step 7: Hybridize Probes (NRCC v2.0)

  • Master Step Name = Hybridize with Pooling v2.0

  • Step Type = Pooling

  • Aliquot Generation = Fixed, 1

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Pooling

  • Label Uniqueness = On

  • Defaults

    • Sample Grouping = Group by Containers

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Placement = Enabled

  • Defaults

    • Well Sort Order = Row

    • Placement Pattern = Column

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Record Details

  • Step Data (Master Step Fields)

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Step 8: Capture Hybridized Probes (NRCC v2.0)

  • Master Step Name = Capture Hybridized Probes v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightCalculate Master Mixhashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 9: Perform Second Hybridization (NRCC v2.0)

  • Master Step Name = Perform Second Hybridization v2.0

  • Step Type = No Outputs

  • Reagent Kits

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 10: Perform Second Capture (NRCC v2.0)

  • Master Step Name = Perform Second Capture v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 11: Clean Up Captured Library (NRCC v2.0)

  • Master Step Name = Clean Up v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

  • Naming Convention = {InputItemName}

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 12: Amplify Enriched Library (NRCC v2.0)

  • Master Step Name = Amplify Enriched Library v2.0

  • Step Type = No Outputs

  • Reagent Kits

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 13: Clean Up Amplified Enriched Library (NRCC v2.0)

  • Master Step Name = Clean Up v2.0

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

  • Naming Convention = {InputItemName}

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Automations

chevron-rightSet Next Step - Advancehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

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Step 14: Bioanalyzer QC (Library Validation) (NRCC v2.0)

  • Master Step Name = Bioanalyzer QC (Library Validation) v2.0

  • Step Type = Standard QC

  • Measurement Generation = Fixed, 1

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Automations

chevron-rightGenerate Bioanalyzer Input filehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon entry

chevron-rightParse Bioanalyzer XML, Copy nM and Assign QC flagshashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Output PASS/FAILhashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightParse Bioanalyzer XML, Assign QC flags, and Copy Concentrationshashtag
  • Trigger Location = Not Used

chevron-rightParse Bioanalyzer XML and assign QC flagshashtag
  • Trigger Location = Not Used

chevron-rightParse Bioanalyzer XML, Calculate nM and assign QC flagshashtag
  • Trigger Location = Not Used

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Placement = Enabled

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Column

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Record Details

Group of Defaults

chevron-rightNextera DNA Flex Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightNextera Mate Pair Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightNextera XT DNA Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightNRCC Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq ChIP-Seq Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTruSeq Exome Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq Methyl Capture EPIC Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq Rapid Exome Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq RNA Access Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq RNA Exome Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSeq Small RNA Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTruSeq Stranded mRNA Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTruSeq Stranded Total RNA Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTruSeq Targeted RNA Expression Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Peak 2 Size - bp

chevron-rightTruSight Myeloid Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTruSight RNA Fusion Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

chevron-rightTSCA Library Validationhashtag
  • Criteria 1 - Operator = >=

  • Criteria 1 - Source Data Field = Region 1 Average Size - bp

  • Step Data

    • Group of Defaults = NRCC Library Validation

    • Master Step Fields

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Step 15: Normalize Libraries (NRCC v2.0)

  • Master Step Name = Normalize Libraries 1 v2.0.10

  • Step Type = Standard

  • Derived Sample Generation = Fixed, 1

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The version of Normalized Libraries 1 master step name may be different depending on the version of IPP installed.

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Automations

chevron-rightNormalization Calculations - Option 1hashtag
  • Trigger Location = Record Details

  • Trigger Style = Manual button

chevron-rightSet Next Step - Removehashtag
  • Trigger Location = Record Details

  • Trigger Style = Automatic upon exit

chevron-rightRouting script - Normalize Librarieshashtag
  • Trigger Location = Step

  • Trigger Style = Automatic upon exit

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Queue/Ice Bucket

  • Defaults

    • Sample Grouping = Group by Containers

    • Well Sort Order = Row

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Record Details

  • Step Data (Master Step Fields)

  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Placement Pattern = Row
  • Destination Containers

    • 96 well plate

  • Step File Placeholders
    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName} Bioanalyzer
    Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Placement Pattern = Column
  • Destination Containers

    • BioAnalyzer DNA High Sensitivity Chip

    • BioAnalyzer DNA 1000 Chip

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Use strict matching for Bioanalyzer results = No

  • Criteria 1 - Threshold Value = 100.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Use strict matching for Bioanalyzer results = No

  • Criteria 1 - Threshold Value = 5.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Molarity

  • Criteria 2 - Threshold Value = 10.00

  • Use strict matching for Bioanalyzer results = No

  • Criteria 1 - Threshold Value = 100.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 300.00

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Use strict matching for Bioanalyzer results = No

  • Options

    Additional Options and Dropdown Items

    Criteria 1 - Operator

    Text Dropdown

    Presets

    • >=

    • <=

    • =

    Criteria 1 - Source Data Field

    Text Dropdown

    Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 1 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Criteria 2 - Operator

    Text Dropdown

    Presets

    • >=

    • <=

    • =

    Criteria 2 - Source Data Field

    Text Dropdown

    Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 2 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Use strict matching for Bioanalyzer results

    Toggle Switch

    Default = None Set

  • Step File Placeholders

    • Bioanalyzer Driver File - Automatically attached

    • Bioanalyzer Driver File Generation Log File - Automatically attached

    • Bioanalyzer XML Result File (required) - Manually uploaded

    • Result File (optional) - Manually uploaded

    • PDF Summary File (optional) - Manually uploaded

    • Bioanalyzer XML Parsing Log File - Automatically attached

    • QC Assignment Log File - Automatically attached

    • QC Assignment Report - Automatically attached

  • Sample Table

    • Enable QC Flags = Yes

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • File Column Options

      • File Column Display = Hide

      • File Attachment Method = Auto

    • Table Columns - Global Fields

  • Naming Convention = {SubmittedSampleName}
  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Default = NLM AMP

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName} Bioanalyzer
    Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Placement Pattern = Column
  • Destination Containers

    • BioAnalyzer DNA High Sensitivity Chip

    • BioAnalyzer DNA 1000 Chip

  • Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 1 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Criteria 2 - Operator

    Text Dropdown

    Custom Entries

    Presets

    • >=

    • <=

    • =

    Criteria 2 - Source Data Field

    Text Dropdown

    Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 2 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Use strict matching for Bioanalyzer results

    Toggle Switch

    Default = None Set

  • Step File Placeholders

    • Bioanalyzer Input File - Automatically attached

    • Bioanalyzer Input File Generation Log File - Automatically attached

    • Bioanalyzer XML Result File (required) - Manually uploaded

    • Result File (optional) - Manually uploaded

    • PDF Summary File (optional) - Manually uploaded

    • Bioanalyzer XML Parsing Log File - Automatically attached

    • QC Assignment Log File - Automatically attached

    • QC Assignment Report - Automatically attached

  • Sample Table

    • Enable QC Flags = Yes

    • Sample Display Default = Expand

    • Well Sort Order = Column

    • File Column Options

      • File Column Display = Hide

      • File Attachment Method = Auto

    • Table Columns - Global Fields

  • Naming Convention = {PoolName}
  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 4

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Well Sort Order = Row
    Destination Containers
    • 96 well plate

    Presets

    • RNA HYB

    • NRC HYB

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Step File Placeholders
    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Nextera Rapid Capture Custom Enrichment Kit, Box 2
    • Supplier = Illumina

    • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

    • Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

  • Nextera Rapid Capture Custom Enrichment Kit, Box 4

    • Supplier = Illumina

    • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

    • Website =

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Default = NRC HYB

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Step File Placeholders
    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Nextera Rapid Capture Custom Enrichment Kit, Box 2
    • Supplier = Illumina

    • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

    • Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    Presets

    • AMP10

    • AMP12

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Reagent Kits

    • Nextera Rapid Capture Custom Enrichment Kit, Box 1

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

      • Website =

    • Nextera Rapid Capture Custom Enrichment Kit, Box 2

      • Supplier = Illumina

      • Catalog Number = FC-140-1007; FC-140-1008; FC-140-1009

  • Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName} Bioanalyzer
    Sample Table (Column Headers)

    Category

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Container

    Container Name

    Built-in

    Container

    LIMS ID (Container)

    Built-in

    Placement Pattern = Column
  • Destination Containers

    • BioAnalyzer DNA High Sensitivity Chip

    • BioAnalyzer DNA 1000 Chip

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,500.00

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Average Size - bp

  • Criteria 2 - Threshold Value = 400.00

  • Criteria 1 - Threshold Value = 250.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Criteria 1 - Threshold Value = 350.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Average Size - bp

  • Criteria 2 - Threshold Value = 400.00

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 1,000.00

  • Criteria 1 - Threshold Value = 200.00

  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 300.00

  • Criteria 1 - Threshold Value = 200.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 500.00

  • Criteria 1 - Threshold Value = 200.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 320.00

  • Criteria 1 - Threshold Value = 200.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 320.00

  • Criteria 1 - Threshold Value = 100.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Average Size - bp

  • Criteria 2 - Threshold Value = 200.00

  • Criteria 1 - Threshold Value = 250.00

  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Average Size - bp

  • Criteria 2 - Threshold Value = 275.00

  • Criteria 1 - Threshold Value = 250.00

  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Average Size - bp

  • Criteria 2 - Threshold Value = 275.00

  • Criteria 1 - Threshold Value = 100.00

  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Peak 2 Size - bp

  • Criteria 2 - Threshold Value = 300.00

  • Criteria 1 - Threshold Value = 150.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Size - bp

  • Criteria 2 - Threshold Value = 400.00

  • Criteria 1 - Threshold Value = 160.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Size - bp

  • Criteria 2 - Threshold Value = 700.00

  • Criteria 1 - Threshold Value = 300.00
  • Criteria 2 - Operator = <=

  • Criteria 2 - Source Data Field = Region 1 Size - bp

  • Criteria 2 - Threshold Value = 400.00

  • Options

    Additional Options and Dropdown Items

    Criteria 1 - Operator

    Text Dropdown

    Custom Entries

    Presets

    • >=

    • <=

    • =

    Criteria 1 - Source Data Field

    Text Dropdown

    Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 1 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Criteria 2 - Operator

    Text Dropdown

    Custom Entries

    Presets

    • >=

    • <=

    • =

    Criteria 2 - Source Data Field

    Text Dropdown

    Presets

    • Concentration

    • Conc. Units

    • Number of Peaks found

    Criteria 2 - Threshold Value

    Numeric

    Decimal Places Displayed = 2

    Use strict matching for Bioanalyzer results

    Toggle Switch

    Default = None Set

  • Step File Placeholders

    • Bioanalyzer Input File - Automatically attached

    • Bioanalyzer Input File Generation Log File - Automatically attached

    • Bioanalyzer XML Result File (required) - Manually uploaded

    • Result File (optional) - Manually uploaded

    • PDF Summary File (optional) - Manually uploaded

    • Bioanalyzer XML Parsing Log File - Automatically attached

    • QC Assignment Log File - Automatically attached

    • QC Assignment Report - Automatically attached

  • Sample Table

    • Enable QC Flags = Yes

    • Sample Display Default = Expand

    • Well Sort Order = Column

    • File Column Options

      • File Column Display = Hide

      • File Attachment Method = Auto

    • Table Columns - Global Fields

  • Naming Convention = {InputItemName}
    ℹ The field value and actual version of the workflows and steps in the routing automation script may be different depending on the version of IPP installed.
    Sample Table
    • Column Headers

      Category

      Field Name

      Field Type

      Options

      Additional Options and Dropdown Items

      Container

      Container Name

      Built-in

    • Expanded View Fields

    • Decimal Places Displayed = 2

    Target Normalization (nM)

    Numeric

    Required Field

    • Default = 2

    • Decimal Places Displayed = 2

  • Step File Placeholders

    • Log File - Automatically attached

  • Sample Table

    • Sample Display Default = Expand

    • Well Sort Order = Row

    • Table Columns - Global Fields

      ℹ The preset options for Derived Sample Sequencing Instrument may vary depending on the version of the IPP.

  • Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    80% EtOH Prep Date

    Date

    Field Name

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Thermal Cycler Program

    Text

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    80% EtOH Prep Date

    Date

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Criteria 1 - Operator

    Text Dropdown

    Custom Entries

    Presets

    • >=

    • <=

    • =

    • !=

    Criteria 1 - Source Data Field

    Text Dropdown

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Thermal Cycler Program

    Text Dropdown

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Thermal Cycler Program

    Text

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    80% EtOH Prep Date

    Date

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Thermal Cycler Program

    Text Dropdown

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    80% EtOH Prep Date

    Date

    Field Name

    Field Name

    Field Type

    Options

    Additional Options and Dropdown Items

    Comment

    Multiline Text

    Final Volume (ul)

    Numeric

    Field Type

    Custom Entries

    • Required Field

    • Custom Entries

    Field Type

    Required Field

    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Sample Volume (ul):: = 50 / input.::Concentration:: ; output.::Buffer Volume (ul):: = 10 - output.::Sample Volume (ul)::' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Sample Volume (ul):: = (0.2 * 5) / input.::Concentration:: ; output.::Buffer Volume (ul):: = 5 - output.::Sample Volume (ul)::' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar -u {username} -p {password} \
          script:driver_file_generator \
            -i {processURI:v2} \
            -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv \
            -o {compoundOutputFileLuid0}.csv \
            -l {compoundOutputFileLuid1}  \
          && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} \
          script:addBlankLines \
            -i {stepURI:v2} \
            -f {compoundOutputFileLuid0}.csv \
            -l {compoundOutputFileLuid1} \
            -sep COMMA \
            -b ',False,' \
            -h 1 \
            -c LIMSID \
            -pre 'Sample '"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {processURI:v2} \
          script:parseBioAnalyzer \
            -inputFile {compoundOutputFileLuid2} \
            -log {compoundOutputFileLuid5} \
            -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' \
          script:assignQC \
            -log {compoundOutputFileLuid6} \
            -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t true \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -excludeControls true -exp 'if (output.QC == true) { nextStep = ::ADVANCE:: } else { nextStep = ::ESCALATE:: }' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'nextStep = ::ADVANCE:: ; input.::Concentration (ng/ul):: = output.::Concentration::' -log {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar script:driver_file_generator -i {processURI:v2} -u {username} -p {password} -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv -o {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1}  && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar script:addBlankLines -i {stepURI:v2} -u {username} -p {password} -f {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1} -sep COMMA -b ',False,' -h 1 -c LIMSID -pre 'Sample '"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; output.::Molarity (nM):: = (output.::Concentration:: * 1000000) / (660 * output.::Region 1 Average Size - bp::) ; input.::Molarity (nM):: = output.::Molarity (nM):: ; output.::Conc. Units:: = ::ng/ul::' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -excludeControls true -exp 'if (output.QC == true) { nextStep = ::ADVANCE:: } else { nextStep = ::ESCALATE:: }' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; input.::Concentration:: = output.::Concentration:: ; output.::Conc. Units:: = ::ng/ul:: ; input.::Conc. Units:: = output.::Conc. Units::' -log {compoundOutputFileLuid8}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'if (output.::Conc. Units::.contains(::pg::)) {output.::Molarity (nM):: = output.::Region 1 Molarity:: / 1000} else {output.::Molarity (nM):: = output.::Region 1 Molarity::} ; (input.::Molarity (nM):: = output.::Molarity (nM)::) ' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2:http} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'step.::Total Number of Samples:: = step.::Total Number of Samples:: + 1' -log {compoundOutputFileLuid0} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2:http} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'step.::Enrichment Elution Buffer 1 (ul):: = 28.5 * step.::Total Number of Samples:: ; step.::2N NaOH (ul):: = 1.5 * step.::Total Number of Samples::' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} \
          script:evaluateDynamicExpression \
          -t false \
          -h false \
          -exp 'nextStep = ::ADVANCE::' \
          -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/DriverFileGenerator.jar script:driver_file_generator -i {processURI:v2} -u {username} -p {password} -t /opt/gls/clarity/extensions/ngs-common/v5/EPP/conf/readonly/bioA_driver_file_template.csv -o {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1}  && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar script:addBlankLines -i {stepURI:v2} -u {username} -p {password} -f {compoundOutputFileLuid0}.csv -l {compoundOutputFileLuid1} -sep COMMA -b ',False,' -h 1 -c LIMSID -pre 'Sample '"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'if (output.::Conc. Units::.contains(::pg::)) {output.::Molarity (nM):: = output.::Region 1 Molarity:: / 1000} else {output.::Molarity (nM):: = output.::Region 1 Molarity::} ; (input.::Molarity (nM):: = output.::Molarity (nM)::) ' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -excludeControls true -exp 'if (output.QC == true) { nextStep = ::ADVANCE:: } else { nextStep = ::ESCALATE:: }' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; input.::Concentration:: = output.::Concentration:: ; output.::Conc. Units:: = ::ng/ul:: ; input.::Conc. Units:: = output.::Conc. Units::' -log {compoundOutputFileLuid8}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:parseBioAnalyzer -inputFile {compoundOutputFileLuid2} -log {compoundOutputFileLuid5} -configFile '/opt/gls/clarity/extensions/conf/v5/bioanalyzer/defaultBioAnalyzerDNAConfig.groovy' && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Concentration:: = output.::Region 1 Conc.:: ; output.::Molarity (nM):: = (output.::Concentration:: * 1000000) / (660 * output.::Region 1 Average Size - bp::) ; input.::Molarity (nM):: = output.::Molarity (nM):: ; output.::Conc. Units:: = ::ng/ul::' -log {compoundOutputFileLuid8} && /opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {processURI:v2} -u {username} -p {password} script:assignQC -log {compoundOutputFileLuid6} -qcResult {compoundOutputFileLuid7}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t true -h false -exp 'output.::Molarity (nM):: = input.::Molarity (nM):: ; if (output.::Molarity (nM):: <= step.::Target Normalization (nM)::) {output.::Sample Volume (ul):: = step.::Final Volume (ul):: ; output.::Buffer Volume (ul):: = 0 ; output.::Normalized Molarity (nM):: = output.::Molarity (nM)::} else {output.::Sample Volume (ul):: = (step.::Target Normalization (nM):: * step.::Final Volume (ul):: ) / input.::Molarity (nM):: ; output.::Buffer Volume (ul):: = step.::Final Volume (ul):: - output.::Sample Volume (ul):: ; output.::Normalized Molarity (nM):: = step.::Target Normalization (nM)::}' -log {compoundOutputFileLuid0}"
    bash -l -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -i {stepURI:v2} -u {username} -p {password} script:evaluateDynamicExpression -t false -h false -exp 'nextStep = ::REMOVE::' -log {compoundOutputFileLuid0}"
    bash -c "/opt/gls/clarity/bin/java -jar /opt/gls/clarity/extensions/ngs-common/v5/EPP/ngs-extensions.jar -u {username} -p {password} -i {stepURI:v2} -l {compoundOutputFileLuid0} script:changeWorkflow \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'MiSeq' \
    --WORKFLOW 'MiSeq Sequencing v3.2' \
    --STEP 'Library Pooling (MiSeq v3.2)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NextSeq' \
    --WORKFLOW 'NextSeq 500/550 Sequencing v1.2' \
    --STEP 'Library Pooling (NextSeq 500/550 v1.2)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NovaSeq 2.0' \
    --WORKFLOW 'NovaSeq 6000 v2.3' \
    --STEP 'Define Run Format (NovaSeq 6000 v2.3)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NovaSeq 3.0' \
    --WORKFLOW 'NovaSeq 6000 v3.8' \
    --STEP 'Define Run Format (NovaSeq 6000 v3.8)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NovaSeqDx' \
    --WORKFLOW 'NovaSeqDx v1.2' \
    --STEP 'Define Run Format (NovaSeqDx v1.2)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NextSeq 1000/2000' \
    --WORKFLOW 'NextSeq 1000/2000 Sequencing v2.4' \
    --STEP 'Library Pooling and Dilution (NextSeq 1000/2000 Sequencing v2.4)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NovaSeq X Series' \
    --WORKFLOW 'NovaSeq X Series v1.1' \
    --STEP 'Assign Analysis Configuration Template (NovaSeq X Series Sequencing v1.1)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS' \
    \
    --FIELD_NAME 'Sequencing Instrument' \
    --FIELD_VALUE 'NextSeq 1000/2000 On-Prem' \
    --WORKFLOW 'NextSeq 1000/2000 On-Prem Sequencing v1.0' \
    --STEP 'Library Pooling and Dilution (NextSeq 1000/2000 On-Prem Sequencing v1.0)' \
    --INPUTS_OR_OUTPUTS 'OUTPUTS'"
    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Buffer Volume (ul)

    Numeric

    Decimal Places Displayed = 2

    Derived Sample

    Sample Name

    Built-in

    Derived Sample

    Sample Volume (ul)

    Numeric

    Decimal Places Displayed = 2

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Built-in

    Built-in

    Container

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Measurement

    A260/280 ratio

    Numeric

    Decimal Places Displayed = 2

    Measurement

    BA Sample Name

    Text

    Measurement

    Concentration

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Conc. Units

    Text

    Measurement

    Number of Peaks found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Number of Regions found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Size - bp

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Sample Comment

    Text

    Project

    Project Name

    Built-in

    Built-in

    Well

    Built-in

    Derived Sample

    Reagent Name

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Built-in

    Measurement

    BA Sample Name

    Text

    Measurement

    Concentration

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Conc. Units

    Text

    Measurement

    Molarity (nM)

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Number of Peaks found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Number of Regions found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Size - bp

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Website = https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right

    Built-in

    Well

    Built-in

    Derived Sample

    Sample Name

    Built-in

    Project

    Project Name

    Built-in

    Built-in

    Measurement

    BA Sample Name

    Text

    Measurement

    Concentration

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Conc. Units

    Text

    Measurement

    Molarity (nM)

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Number of Peaks found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Number of Regions found

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 1 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 2 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 3 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 4 Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Peak 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Peak 5 Size - bp

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 1 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 1 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 2 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 2 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 3 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 3 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 4 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 4 Molarity

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Average Size - bp

    Numeric

    Decimal Places Displayed = 0

    Measurement

    Region 5 Conc.

    Numeric

    Decimal Places Displayed = 2

    Measurement

    Region 5 Molarity

    Numeric

    Decimal Places Displayed = 2

    Built-in

    Well

    Built-in

    Derived Sample

    Buffer Volume (ul)

    Numeric

    Decimal Places Displayed = 2

    Derived Sample

    Molarity (nM)

    Numeric

    Decimal Places Displayed = 2

    Derived Sample

    Normalized Molarity (nM)

    Numeric

    Decimal Places Displayed = 2

    Derived Sample

    Sample Name

    Built-in

    Derived Sample

    Sample Volume (ul)

    Numeric

    Decimal Places Displayed = 2

    Derived Sample

    Sequencing Instrument

    Text Dropdown

    Required Field

    Presets

    • MiSeq

    • NextSeq

    • NextSeq 1000/2000

    Project

    Project Name

    Built-in

    !=

    Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

  • Peak 2 Conc.

  • Peak 2 Molarity

  • Peak 3 Size - bp

  • Peak 3 Conc.

  • Peak 3 Molarity

  • Peak 4 Size - bp

  • Peak 4 Conc.

  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

  • !=

    Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

  • Peak 2 Conc.

  • Peak 2 Molarity

  • Peak 3 Size - bp

  • Peak 3 Conc.

  • Peak 3 Molarity

  • Peak 4 Size - bp

  • Peak 4 Conc.

  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

  • Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

  • Peak 2 Conc.

  • Peak 2 Molarity

  • Peak 3 Size - bp

  • Peak 3 Conc.

  • Peak 3 Molarity

  • Peak 4 Size - bp

  • Peak 4 Conc.

  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

  • !=

    Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

  • Peak 2 Conc.

  • Peak 2 Molarity

  • Peak 3 Size - bp

  • Peak 3 Conc.

  • Peak 3 Molarity

  • Peak 4 Size - bp

  • Peak 4 Conc.

  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

  • !=

    Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

  • Peak 2 Conc.

  • Peak 2 Molarity

  • Peak 3 Size - bp

  • Peak 3 Conc.

  • Peak 3 Molarity

  • Peak 4 Size - bp

  • Peak 4 Conc.

  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

  • !=

    Peak 1 Size - bp

  • Peak 1 Conc.

  • Peak 1 Molarity

  • Peak 2 Size - bp

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  • Peak 4 Molarity

  • Peak 5 Size - bp

  • Peak 5 Conc.

  • Peak 5 Molarity

  • Number of Regions found

  • Region 1 Average Size - bp

  • Region 1 Conc.

  • Region 1 Molarity

  • Region 2 Average Size - bp

  • Region 2 Conc.

  • Region 2 Molarity

  • Region 3 Average Size - bp

  • Region 3 Conc.

  • Region 3 Molarity

  • Region 4 Average Size - bp

  • Region 4 Conc.

  • Region 4 Molarity

  • Region 5 Average Size - bp

  • Region 5 Conc.

  • Region 5 Molarity

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    https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right
    https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right
    https://support.illumina.com/sequencing/sequencing_kits/nextera-rapid-capture-custom-kit.htmlarrow-up-right
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    NextSeq 1000/2000 On-Prem

  • NovaSeq 2.0

  • NovaSeq 3.0

  • NovaSeq X Series

  • NovaSeqDx