DRAGEN Enrichment

There are multiple ways that the DRAGEN Enrichment workflow can be run. Please follow the appropriate instructions for the desired workflow:

• Somatic High Coverage

• Somatic Medium Coverage

• Somatic Low Coverage

• Enrichment Germline

Coverage Rate Minimums

The following coverage rates are the minimums recommended for each of the various workflows:

• High Coverage

  • Post-Collapsed Coverage Rate >2000x (liquid tumor mode)

• Medium Coverage

  • Post-Collapsed Coverage Rate 200-300x (solid tumor mode)

• Low Coverage

  • Coverage Rate >20x for Germline Methyl-Only

  • Coverage Rate >30x for Germline Methyl + VC

  • Coverage Rate >100x for Somatic Methyl + VC

If the UMI-collapsed reads do not meet the recommended post-collapsed coverage depths for the solid tumor or liquid biopsy pipelines, then it is recommended to run with the default UMI settings