May 2024
Adjustable thresholds to determine pass/fail status
Data summary plots for a quick visual check of each analysis batch
Determining detection p-value, beta-values, and m-values from each methylation sample
Deployment on BaseSpace™ Sequence Hub user interface for easy analysis kickoff
Adjustable thresholds for 21 built in controls, p-value detection, proportion probes passing, and offset correction within BaseSpace Sequence Hub to customize for user’s study needs
Thresholds are used to assign pass (1) or fail (0) status to each sample
Failed metrics can be highlighted for easy viewing
Pinpoint areas of failure including bisulfite conversion, staining, hybridization, etc. to identify assay steps in need of troubleshooting
Quantitative values for each control removing ambiguity with manual interpretation
Data summary plots with information on passing p-value detection and principal component analysis of beta values
Provides detection p-value, beta-values and m-values for each CG site per sample to use in downstream analysis
Standard thresholds may not be applicable for all discontinued, semi-custom or custom BeadChips and IDATs originating from NextSeq550
Built-in controls may not be available on all discontinued, semi-custom or custom BeadChips