# Data Management ## Copying data to local /staging drive * Copy the run or FASTQ folder to the DRAGEN server into the staging folder with the following recommended organization: `/staging/runs/{RunID}`. You can copy the run folder onto the DRAGEN server using Linux commands such as `rsync`. The sample sheet within the run folder is used unless otherwise specified through the command line. * Run folder must be intact. * If the analysis output folder path is different from the default, provide the analysis output folder path. ### Analysis output directory Before running the analysis, confirm that the output directory for the software to write to is empty and does not include results of previous analyses. ### Storage Requirements The DRAGEN server provides an NVMe SSD in the /staging directory to use as the software output directory. Network-attached storage is required for long-term storage. When running the Heme pipeline, use the default settings or set the -analysisFolder command line option to a directory in /staging to make sure the DRAGEN server processes read and write data on the NVMe SSD. Before beginning analysis, develop a strategy to copy data from the DRAGEN server to a network‑attached storage. Delete output data on the DRAGEN server as soon as possible. The following are the run folder output size estimates and the minimum free space requirements for fastq.gz or fastq.ora output format. | Sequencing System | Run Folder Output (Gb) | Minimum Disk Space .gz (Gb) | Minimum Disk Space .ora (Gb) | | -------------------------------------- | ---------------------- | --------------------------- | ---------------------------- | | NovaSeq 6000/6000Dx (RUO) S4 Flow Cell | \~2000 | 4000 | 2500 | | NovaSeq X 10B | \~2000 | 4000 | 2500 | | NovaSeq X 25B | \~4250 | 8500 | 5300 | | Other Instruments | \~2000 | 4000 | 2500 | When launching the analysis, the software checks that the minimum disk space required is available. If the minimum disk space is not available, the software shows an error message and prevents analysis from starting. If disk space is exhausted during a run, the run shows an error and stops analyzing. Moving or modifying files during an analysis may cause the analysis to fail or provide incorrect results. ## Data streaming from Network Filesystem Analysis of data stored on network file system may be slow when there are multiple DRAGEN servers reading and writing to the network file system simultaneously. However, it is advisable to use a network filesystem to stream large datasets from NFS when data transfer to local /staging is taking a significant amount of time, especially for NovaSeq X 25B flow cells. Discuss with your system administrator for [Network Considerations](https://support.illumina.com/content/dam/illumina-support/documents/documentation/software_documentation/dragen-bio-it/200015717_03_dragen-server-v4-site-prep-and-installation-guide.pdf) of the DRAGEN server. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.connected.illumina.com/dragen-clinical-research-apps/readme/common/server/advanced/data-storage.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.