Introduction

The DRAGEN miRNA software is integral to the Secondary Analysis step in Illumina's miRNA prep End-to-End (E2E) solution, accessible via the BaseSpace Sequence Hub (BSSH) and Illumina Connected Analytics (ICA) cloud platforms. This section aims to further illustrate how counts align within the workflow and to outline the other components of the E2E solution (see Figure 1).

Figure 1. Illumina miRNA prep E2E worfklow.

Illumina miRNA E2E workflow

Once samples are prepared with Illumina miRNA prep and sequenced, customers can proceed with secondary analysis. Multiple samples can be entered into the pipeline. During the BSSH Run Planning phase, a samplesheet must be created and either uploaded or selected on the sequencer during setup. After sequencing, use the BCLConvert BSSH app or a local tool to demultiplex samples, producing FASTQ files. Secondary analyses, such as counts, QC, and reports, can be manually initiated via ICA or BSSH using FASTQs as inputs. Count matrix files and DRAGEN Reports are available for download through BaseSpace or ICA, depending on your subscription type.