Sample Sheet Creation

Before launching the DRAGEN miRNA app on Illumina's cloud platforms (BSSH or ICA), ensure that samples are demultiplexed and FASTQ files are created. Use BCLConvert locally or on the cloud for this purpose.

To run BCLConvert a sample sheet is required. A sample sheet is a comma-separated value (*.csv) file format used by Illumina instruments, platforms, and analysis pipelines to store settings and data for sequencing and analysis. For general information on the v2 sample sheet, refer to Illumina Connected Software - Sample Sheet.

To create a sample sheet compatible with BCLConvert using the BSSH Run Planner, follow these steps:\

1. Select the Runs tab, and then select the New Run drop-down.

2. Select Run Planning.

3. Run Settings wizard will be loaded.

4. In the Run Name field, enter a unique name of your preference to identify the current run. The run name can contain a maximum of 255 alphanumeric characters, spaces, dashes, and underscores.

5. [Optional] In the Run Description field, enter a description of the current run. The run description can contain a maximum of 255 alphanumeric characters.

6. Select the Instrument Platform (one of the following: NovaSeq 6000/6000Dx, NextSeq 1000/2000, NextSeq 500/550, MiniSeq, MiSeq or iSeq 100).

7. Select the analysis location. Depending on the selected instrument type, not all options may be available.

   • BaseSpace - Analyze sequencing data in the cloud.

   • Local - Analyze sequencing data on-instrument or generate a Sample Sheet v2 for Local or Hybrid mode.

8. [Optional] In the Library Tube ID field, optionally enter the library tube ID of the current run. The library tube id can contain a maximum of 255 alphanumeric characters.

9. Select Next

10. Configuration wizard will be loaded

11. Select an analysis type and version. Choose the latest version of BCL Convert (e.g., DRAGEN BCL Convert 4.3.13)

12. Select the following instrument settings. Depending on the library prep kit, recommended options are automatically selected. Some library prep kits have hard-coded number of indexes reads and read types, which cannot be changed.

    • Library prep kit: Library miRNA prep

    • Index adapter kit: one of the following: Illumina miRNA UDI Indexes Set A, B, C, or D

    • Number of index reads: 2 indexes

    • Read type: Single Read

    • Read Lengths:

      • Read 1: 72

      • Index 1: 10

      • Index 2: 10

      • Read 2: 0

    • Override Cycles:

      • Read 1: Y72

      • Index 1: I10

      • Index 2: I10

For more information on different instruments, refer to Illumina support documentation.