# Analysis in ICA

### Overview

This tutorial guides you through running the Illumina DRAGEN miRNA pipeline using [Illumina Connected Analytics](https://help.ica.illumina.com/). You'll begin by setting up a project with demultiplexed instrument run data (FASTQ), adding pipelines and running a pipeline.

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### Set-up <a href="#set-up" id="set-up"></a>

This tutorial assumes you already have an existing project in ICA. To create a new project, please see instructions in the [Projects](https://help.ica.illumina.com/home/h-projects#create-new-project) page.

Additionally, you will need the Illumina DRAGEN miRNA Bundle linked to your existing ICA project. The DRAGEN miRNA Demo Bundle is an an [entitled bundle](https://help.ica.illumina.com/home/h-bundles#access-an-entitled-bundle) provided by Illumina with all standard ICA subscriptions and includes DRAGEN pipelines, references, and demo data.

#### Linking an Bundle <a href="#linking-an-entitled-bundle" id="linking-an-entitled-bundle"></a>

For general steps on creating and linking bundles to your project, see the [Bundles](https://help.ica.illumina.com/home/h-bundles) page. This tutorial explores the DRAGEN miRNA Published Pipeline, so we will need to link the DRAGEN miRNA Bundle to our existing project.

Steps:

* Go to the **Projects > your\_project > Project settings > Details** page
* Click **Edit**
* Click the **+** button, under Linked bundles
* Select the *DRAGEN miRNA 1.0.0 Bundle*, followed by **Link**.
* Finally, **save** the change.

DRAGEN miRNA 1.0.0 Bundle assets will now be available on the *Data* and *Pipelines* pages.

### Pipelines <a href="#pipelines" id="pipelines"></a>

After setting up the project in ICA and linking a bundle, we can run various pipelines.

#### DRAGEN miRNA Published Pipeline <a href="#dragen-germline-published-pipeline" id="dragen-germline-published-pipeline"></a>

This example demonstrates how to run the DRAGEN\_miRNA\_1-0-0 Published Pipeline in your ICA project using the demo data from the linked DRAGEN miRNA 1.0.0 Bundle.

The required pipeline input assets for this tutorial include:

* Under **Projects > your\_project > Data**
  * Illumina DRAGEN miRNA Demo Data folder
* Under **Projects > your\_project > Flow > Pipelines**
  * Illumina DRAGEN miRNA

#### **Launching the Illumina DRAGEN miRNA Published Pipeline with Demo Data**

From the **Projects > your\_project > Flow > Pipelines** page, select Illumina DRAGEN\_miRNA\_1-0-&#x30;*,* and then click **Start analysis.** Initial set-up details require pipeline run name meaningful to the user and a *Subscription* from the drop-down menu under Pricing.

Running the Illumina DRAGEN miRNA pipeline uses the following inputs which are to be added in the Input Files section:

**FASTQ files**

Select the FASTQ files in the *Illumina DRAGEN Enrichment Demo Data* folder and select **Add**.

**Reference**

Select the reference database to be used for mapping and annotation. The database name indicates the collection of reference files used for small RNA classification and is based on the corresponding miRBase version. Default Reference is set to "miRBase\_v22".

**Species**

Select the species of interest. Libraries from six model organisms are available for miRNA classification. Default species is set to "Human".

**Resources**

Choose the expected storage space required for storing analysis results.