# Introduction

End to End (E2E) [Illumina Protein Prep](https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/protein-prep.html) (IPP) workflow combines Illumina chemistry, SOMAmer technology, and DRAGEN data analysis for a comprehensive, automated NGS-based proteomics solution. This E2E solution provides the following:

* NGS readout of more than 9.5K unique protein targets for a single plasma or serum sample.
* From sample to processed results in under 2.5 days with just 4 hours of hands-on time.
* Integrated analysis via [BaseSpace Sequence Hub](https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub.html) (BSSH), [Illumina Connected Analytics](https://www.illumina.com/products/by-type/informatics-products/connected-analytics.html) (ICA), and [Illumina Connected Multiomics](https://help.multiomics.illumina.com/icm) (powered by [Partek](https://help.partek.illumina.com/)).
  * Includes both local and cloud solutions for planning a run and for processing data with DRAGEN Protein Quantification.

## End-to-End Overview

The E2E Illumina Protein Prep solution integrates automation steps to perform sample preparation, protein capture, sequencing, and bioinformatics analysis. Once sequencing is finished, the DRAGEN Protein Quantification application automatically initiates in BSSH or ICA. The diagram below illustrates the E2E workflow.

<figure><img src="https://958780164-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FnyQb4WG1K4VQZKovLv5v%2Fuploads%2Fgit-blob-2a7f9022b8c7b060a2b6abf4e9f6c250c3db9cd1%2FScreenshot%202025-02-12%20at%205.22.40%E2%80%AFPM.png?alt=media" alt=""><figcaption><p>E2E Illumina Protein Prep (IPP) workflow.</p></figcaption></figure>

For documentation on the assay and automation components of Illumina Protein Prep, please refer to the Illumina Protein Prep Product Documentation (document # 200045446).

## Versioning

Unless otherwise specified, this documentation covers DRAGEN Protein Quantification v2.3.0.


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