Output Structure

DRAGEN Protein Quantification produces the following key output files in BaseSpace Sequence Hub:

• DRAGEN_Protein_Quantification_<SW Version>

  • <project> (if no projects are provided, there shall be one folder titled with the RunName)

    • adat

      • <output file prefix>_Step3_SampleNorm.adat: This file contains the final normalized counts for the samples in this project.
      • csv_format: This folder contains the CSV version of the ADAT described above.
      • other_normalization_steps: This folder contains all raw count and intermediate normalized ADATs with their respective counts.
      • control_only: This folder contains control-only ADATs that include counts from calibrator, QC, and blank samples only.
    • quality metrics

      • <output file prefix>_run_qc_stats.csv: This file provides a summary of per sample run qc statistics.
    • DRAGEN Report
• DRAGEN Report: Folder containing all projects DRAGEN report file/s
• Extra Files
• BioInsight Platform Core Logs: Folder containing information on Platform Core logs

Example of Output Structure in BSSH

The example below contains an analysis configured with two Projects, "Project1" and "Project2". Analysis and outputs are processed for each project individually (check Multi-Analysis section for details).

Each project folder (highlighted in yellow) includes "quality_metrics" and "adat" folders, along with a DRAGEN Report. The final normalized counts file (with suffix "_Step3_SampleNorm.adat") is available for each project and can be used in exploratory analysis.