# Known Limitations

**Known Limitations/Issues with DRAGEN Protein Quantification v2.3.0 Software:**

* Only supports 96 samples per PlateBarcode for a single analysis.
* SampleIDs must be unique prior to uploading IPPAS output files to BaseSpace Sequence Hub run planner tool.
* Unable to support AA probe pools runs (as noted by the last two values in the Probe Plate barcode. ie PP201013012345678-AA).
* DRAGEN Reports yield graph always displays 96 samples if possible; if there are fewer than 96 on a single plate, it will pull in samples from the next plate.

**Known Limitations/Issues with Local Sample Sheet Generation Tool v1.0.0:**

* Sample sheet must be saved as "CSV Comma-Delimited" and not "CSV UTF-8".
* Once saved, `LibraryPrepKits` under section `[Sequencing_Settings]` must be set to "IlluminaProteinPrepKit9.5k" for compatibility with the latest SW.


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://help.connected.illumina.com/dragen-protein-quantification/references/known-limitations.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.