> For the complete documentation index, see [llms.txt](https://help.connected.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.connected.illumina.com/dragen-protein-quantification/references/known-limitations.md).

# Known Limitations

**Known Limitations/Issues with DRAGEN Protein Quantification v3.0 Software:**

* Only supports 96 samples per PlateBarcode for a single analysis.
* SampleIDs must be unique prior to uploading SomaSeq Automation System output files to BaseSpace Sequence Hub run planner tool.
* Calibrators provided with MultiMatrix runs are retained in the ADAT but are not used by autolaunch normalization. Explore [DRAGEN Protein Quantification: Advanced Normalization Options in ICM](/dragen-protein-quantification/after-counting-and-normalization/flex-norm.md) to use matched sample-type calibrators.
* Runs from different assay versions (v1 and v2) of SomaSeq Discovery cannot be combined in the same analysis.
* If multiple kit types are present in the same flowcell, separate ADATs are generated for each kit type.
* DRAGEN Protein Quantification v3.0 only supports the following Probe Pool lot combinations: CSF and MultiMatrix kits require Probe Pool AA; Plasma and Serum kits require Probe Pool AB.
* DRAGEN Report specification page for MultiMatrix incorrectly displays the specification range for QC Check (PlatformSpecificQCCheckTailPercent) as 0.8 - 1.2; the correct range applied by the software is 0.4 - 1.6.

**Known Limitations/Issues with Local Sample Sheet Generation Tool v3.0:**

* Sample sheet must be saved as "CSV Comma-Delimited" and not "CSV UTF-8".
* Tool only supports starting from SomaSeq Automation System File v2, supporting SomaSeq Discovery v2 Assay.


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