# Introduction

The DRAGEN Single Cell RNA application is a secondary analysis tool that can process multiplexed single cell RNA-Seq data in binary base call (BCL) files produced by NovaSeq 6000/6000Dx, NextSeq 1000/2000, and NovaSeq X Series sequencing systems to a cell-by-gene expression matrix.

You can perform secondary analysis in the cloud via BaseSpace Sequence Hub or Illumina Connected Analytics (ICA). When performing secondary analysis in the cloud, the analysis application launches automatically in BaseSpace Sequence Hub or ICA after the sequencing workflow completes.


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