Prerequisites

Before you begin, verify that you have the following:

• An Illumina Connected Analytics (ICA) account with a valid subscription (A basic ICA account also provides access to BaseSpace Sequence Hub)

• A positive balance of iCredits for data storage and compute

• A custom panel BED file for your target regions

• At least one workgroup configured in your Illumina account

For information on how to register your ICA subscription and purchase iCredits, refer to the Illumina Connected Analytics setup documentation.

Preparation: Connect BSSH to ICA

Before your first sequencing run, configure your BaseSpace Sequence Hub settings to send sequencing run data to ICA. This one-time setup enables you to use either ICA or BSSH to requeue analysis if needed, and to connect output files to Illumina Connected Insights for downstream interpretation and reporting.

To configure:

1. Log in to BaseSpace as the workgroup owner and select your workgroup.

2. Navigate to Settings (click the workgroup name in the header, then select Settings).

3. Set the ICA Run Storage toggle to ON.

NOTE. This step is not required for NovaSeq X Series instruments, which send data to ICA automatically. If the ICA Run Storage toggle is not visible in your settings, contact Illumina Support at [email protected] to request access.