Connecting to Connected Insights

After the DRAGEN for IDT Custom Panels pipeline completes, you can import the VCF output into Illumina Connected Insights for variant interpretation, annotation, and clinical research reporting.

What Connected Insights Provides

• OncoKB, CKB, and CIViC knowledge bases for biological classification and actionability

• Lab's own My Knowledge Base

• Standard-based oncogenicity prediction

• Somatic/Germline knowledge base matching based on variant origin

• Customizable QC metrics and coverage thresholds

• Customizable TMB threshold per disease group

• Advanced variant filtering and prioritization

• Report automation

• Flexible report templates supporting PDF and DOCX

• Best-in-class visualization, plus IGV support

How to Connect

Illumina Connected Insights is a software solution for interpretation and reporting of NGS data. It accepts VCF files from various analysis pipelines, annotates variants with genomic databases, and generates customized reports.

• If you configured your BaseSpace Sequence Hub (BSSH) settings to send data to Illumina Connected Analytics (ICA) before sequencing, Connected Insights can automatically ingest your results.

• Alternatively, manually import the ICA analysis or ICA folder into Connected Insights.

• For automated workflows, configure a Test Definition in Connected Insights and link it to the DRAGEN pipeline output via the Data Upload settings.

For setup instructions, refer to the Illumina Connected Insights Data Upload (ICA) documentation.

Setup recommendation. Configure your BSSH settings to send sequencing run data to ICA before sequencing. This enables you to use either ICA or BSSH to requeue analysis and to connect output files to Connected Insights. This step is not required for NovaSeq X Series instruments, which send data to ICA automatically. See Prerequisites.