Key VCF Fields

The variant calling pipeline produces a hard-filtered VCF file (*.hard-filtered.vcf.gz). The table below summarizes the most useful fields for somatic variant interpretation.

Metric	VCF Field	Description
Allele Frequency	AF	Fraction of reads supporting the listed variant.
Somatic Quality	SQ	Somatic quality score; higher values indicate stronger evidence.
Systematic Noise Score	AQ	Low values indicate increased probability that a variant is a systematic artifact.
Read Depth	DP	Number of reads at the genomic position.

For complete VCF field definitions, refer to the VCF file header and the DRAGEN Bio-IT Platform documentation.