> For the complete documentation index, see [llms.txt](https://help.connected.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.connected.illumina.com/dragen-stratamap/dragen-spatial-transcriptome/run-setup/per-lane-loading.md).
# Per-lane Loading
**For NovaSeq X Series only,** multiple Illumina spatial transcriptome libraries can be sequenced on the same flow cell if libraries are separated into individual lanes. This page describes how to set up sequencing and analysis when utilizing per-lane loading.
{% hint style="warning" %}
At this time, Illumina spatial transcriptome libraries can **only** be sequenced with other Illumina spatial transcriptome libraries. Do **not** sequence Illumina spatial transcriptome on the same flow cell with any other library type.
{% endhint %}
## Overview
Autolaunch can initiate analysis for one library per sequencing run. Any additional libraries will need to be launched manually in ICA. Alternatively, if autolaunch is not preferred, you may perform a standalone run and manually launch analyses in ICA for all individual libraries.
**Option 1: Autolaunch one analysis, manually launch additional analyses:**
***
**Option 2: Standalone sequencing run and manually launch all analyses:**
***
## Run Planning for Per-lane Loaded Libraries
Regardless of the chosen approach, the Run Planning tool must be used for each library. Each **planned run and sample sheet must contain details for only one library.** Attempting to include multiple slides in a single sample sheet will result in an error.
When using the Run Planning tool, follow the instructions on the [Run Planning page](/dragen-stratamap/dragen-spatial-transcriptome/run-setup/run-planning.md). For Lane Usage, select only the lanes used by the library instead of all lanes. Unspecified lanes will still be sequenced, but their data will be stored for later access and manual launch in ICA.
Example of autolaunch usage in the Run Planning tool for a single library loaded in lanes 3 and 4
### Non-standard read lengths
The recommended sequencing configuration for spatial libraries is 130-8-0-0 (100 cycle sequencing kit), however, if a different configuration is needed to share the flowcell with another library, a manual workaround is possible.
The override\_cycles and override\_reads will need to be adjusted to accommodate the differences. This will depend on the sequencing configuration, but here are some examples:
| 130:8 | I30;N22U7Y71;N8 | I30;Y100;I8 |
| ----------- | ------------------------ | ---------------- |
| 200:8 | I30;N22U7Y93N48;N8 | I30;Y170;I8 |
| 300:8 | I30;N22U7Y93N148;N8 | I30;Y270;I8 |
| 150:8:8:150 | I30;N22U7Y91;N8;N158 | I30;Y120;I8;Y158 |
| 250:8:8:250 | I30;N22U7Y93N98;N8;N258 | I30;Y220;I8;Y258 |
| 300:8:8:300 | I30;N22U7Y93N148;N8;N308 | I30;Y270;I8;Y308 |
To adjust the override\_cycles and override\_reads start the analysis manually in ICA and update the [analysis config file](/dragen-stratamap/dragen-spatial-transcriptome/analysis-inputs/analysis-config-file-optional.md).
Example analysis config file:
#### Some example situations:
{% hint style="info" %}
When sequencing StrataMap libraries, we do not recommend using a read length shorter than 117bp for Read1 for any configuration
{% endhint %}
**Enrichment – Exome (200cycle kit)**
Use the 200cycle sequencing kit with a read length structure of 117-10-10-101 (if enrichment library prep utilizes dual 10bp indices) OR 121-8-8-101 (if enrichment library prep utilizes dual 8bp indices). The Statamap and Enrichment – Exome library preps would need to be loaded in separate lanes.
In the analysis\_config.json file, the override\_cycles and override\_reads must be updated to:
117-10-10-101 read length structure:
* "override\_cycles": "I30;N22U7Y58;N10;N111"
* "override\_reads": "I30;Y87;I10;Y111"
121-8-8-101 read length structure:
* "override\_cycles": "I30;N22U7Y62;N8;N109"
* "override\_reads": "I30;Y91;I8;Y109"
**Whole genome (300cycle kit)**\
The customer would need to use the 300cycle sequencing kit with a read length structure of 151-10-10-151 (if whole genome library prep utilizes dual 10bp indices) OR 151-8-8-151 (if whole genome library prep utilizes dual 8bp indices). The Statamap and Whole genome library preps would need to be loaded in separate lanes.
In the analysis\_config.json file, the override\_cycles and override\_reads must be updated to:
151-10-10-151 read length structure:
* "override\_cycles": "I30;N22U7Y58N34;N10;N161"
* "override\_reads": "I30;Y121;I10;Y161"
151-8-8-151 read length structure:
* "override\_cycles": "I30;N22U7Y58N34;N8;N159"
* "override\_reads": "I30;Y121;I8;Y159"
---
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