Prerequisites

System & Software Requirements

• DRAGEN v4 Server is fully operational and network-connected.
• DRAGEN software v4.5.
• DRAGEN Spatial License

  • License includes the "Spatial Transcriptome" feature.
  • License is not expired.
  • To verify your license, run either one of the following commands:

    sudo dragen_lic -j
    or
    sudo dragen_lic status

    Look for the "Spatial" section with "Active" status.
• Docker 26 or later.
• Oracle Linux 8 operating system image.

  • If needed, upgrade instructions (CentOS 7 → OL8) are available on MyIllumina.
• DRAGEN Application Manager v1.1.7 successfully installed on the DRAGEN server.

  • The run script for the application manager is provided within the installation package.
  • On the first installation of the local application, the installer will detect whether DRAGEN Application Manager is installed. If it is missing or outdated, it will prompt you to install the required version using the provided DRAGEN Application Manager run script.

    • Refer to "Step 2: Install the Application" found on the Installation Page for more information

Permissions

• sudo permissions are required to install the software.
• Elevated permissions are recommended to avoid read/write issues during analysis.

Input & Output Data Access

• You must have a method to transfer input and output files to and from the DRAGEN server.

  • Examples include a mounted network share or a USB drive.

    • Inputs to the pipeline must reside locally on the server.
    • Outputs from the pipeline should also be written locally to prevent transfer issues caused by network connection disruptions.
    • Instructions for configuring file transfers are not provided here. Work with your internal IT team to set this up prior to installation.

Docker does not allow inputs from a mounted network share. Copy all analysis inputs to the server prior to pipeline execution.

Storage & Performance Requirements

• Ensure sufficient storage and memory are available to run a single analysis.

  • It is recommended to keep only one set of input and output files on the server at a given time.

    • Maintain efficient and careful data transfer and removal practices to ensure smooth performance.
  • Input and output data should not be stored on the DRAGEN server long-term.