> For the complete documentation index, see [llms.txt](https://help.connected.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.connected.illumina.com/dragen-stratamap/illumina-spatial-image-tool/tissue-target-test/load-images.md).

# Load Images

The Image Processing workflow requires stitched images. On the **Load images** screen, provide the following inputs:

1. Substrate Information
2. Sample Information

## Substrate Information

Substrate information consists of two fields:

1. `Substrate ID`: A unique identifier for the slide. This value can be found etched on the slide (e.g., 101010DC1).
2. `Substrate label`: Optional, user-defined text to describe the set of samples on the slide (e.g., MicroscopeX\_TissueTargetTest1).

## Sample Information

Sample information is required to name and locate your sample image files. If the slide contains multiple tissue sections, you can add additional samples by specifying the number of rows and clicking **Add rows**.

For each sample, enter:

1. `Sample ID`: A user-defined name for the sample or tissue section (e.g., Tissue Target Test Sample).
2. `Stitched image`: The file path to the stitched image associated with this sample.

### Image Requirements

* Images must be of individual tissue sections. If your imaging system outputs a single image containing multiple sections, use third-party software to crop and save each section as a separate image file.
* Individual tissue image file size should not exceed:
  * 4 GB for computers with 16 GB RAM
  * 8 GB for computers with 32 GB RAM
* Brightfield color images must be saved as:
  * Single-page, lossless (uncompressed) TIFF format
  * 8-bit depth


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