Image Processing

The image processing workflow is a guided six-step process for preparing tissue images for downstream spatial analysis. Each session processes one slide at a time from start to finish.

Image processing is resource intensive, both in terms of RAM and CPU/GPU. While processing time is difficult to estimate, as it depends on a multitude of factors, the RAM requirement is straightforward: for every 1 GB of uncompressed image file size, 4 GB of free RAM is required. It is critical to use a computer with sufficient RAM installed (we recommend 32 GB of RAM) and to close unnecessary applications while using Illumina Spatial Image Tool.

Workflow Navigation

Each step in the workflow includes the following navigation options:

Button	Description
Cancel	Exits the workflow and returns to the homepage. Progress is not saved.
Continue later	Saves progress and exits the workflow. The session can be resumed later.
Back	Returns to the previous step.
Next step	Proceeds to the next step.

Resuming a Workflow

If a workflow session is interrupted or exited using Continue later, progress is saved automatically. The session can be resumed from where it left off when the application is reopened.