Refine Tissue Masks

The Refine tissue masks screen is the fifth step in the image processing workflow. This screen is used to review and edit the tissue mask. Only area in the mask selection will be included in analysis.

Auto-Generated Mask

Auto-generated masks are produced for all samples. If the auto-generated masks are unsatisfactory, users can modify them using the built-in mask editor, or replace them with an externally generated mask by selecting the Actions dropdown, downloading the registered image, using that image to generate the external mask, and then uploading the external mask via the Actions dropdown. Supported formats for the external tissue mask are .tiff and .png.

Product Type

Select the product type that matches the kit being used. The product type selection determines the active area where tissue should be located. If tissue extends outside the well or active area, a warning is displayed and a hatched overlay indicates areas outside the well boundary.

Including area outside the active area of your product may skew summary statistics. It is recommended to crop that area out with the crop to active area tool in the toolbar.

Image Preview

The right side of the screen displays the tissue image with the detected mask overlay. The mask appears as a colored highlight over the tissue region.

Tissue mask editing tools

The toolbar on the right side provides tools for viewing and editing the tissue mask:

Tool	Description
Zoom in	Magnify the image.
Zoom out	Reduce magnification.
Show/hide mask	Toggle the mask overlay visibility.
Draw tool	Manually draw mask regions.
Erase tool	Remove mask regions.
Brush size	Adjusts the width of the paintbrush and eraser.
Show/hide well boundary	Displays hatching if image area is outside the active area of the product.
Crop boundary area	Cuts out the area that is outside the active area of the product.
Undo	Revert the last edit.
Reset	Reset to the auto-detected mask.
Brightness	Adjust image brightness. Note: this is just a tool for mask generation; edits will not appear in the exported image.
Contrast	Adjust image contrast. Note: this is just a tool for mask generation; edits will not appear in the exported image.

Review

Once you are satisfied with the mask for a sample, check the Ready for analysis box. This will lock the mask, so only do this once you have fully finished editing.

If you do not want to include a sample for downstream analysis, leave the Ready for analysis box unchecked for the sample.