Network Requirements

Illumina Connected Software (e.g. BaseSpace Sequence Hub, Illumina Connected Analytics [ICA], and Illumina Connected Multiomics [ICM]) rely on cloud services for login, sequencing, and analysis.

The Illumina Spatial Solutions product utilizes Illumina Connected Software throughout the workflow. The following must be enabled to utilize Illumina cloud software.

• Outbound Connectivity on TCP Port 443:

- All domains listed in this guide require secure (HTTPS) outbound access over TCP port 443.

• Allowed Domains:

- Ensure that any proxy or firewall rules do not block secure connections (HTTPS) to the listed domains.

Common Domains

The following domains are shared across all Illumina services and must always be reachable

• ica.illumina.com
• connected.illumina.com
• login.illumina.com
• *.login.illumina.com

BaseSpace Sequence Hub

Images processed using the Illumina Spatial Image Tool can be directly exported to BaseSpace Sequence Hub where they are selected for subsequent sequencing run planning and analysis steps. To enable this feature, region specific domains must be accessible by your workstation and sequencing instrument.

Region	Domain
All	instruments.sh.basespace.illumina.com
US East (N. Virginia)	api.basespace.illumina.com basespace-data-east.s3.amazonaws.com basespace-data-east.s3.us-east-1.amazonaws.com basespace-data-east.s3-external-1.amazonaws.com stratus-gds-use1.s3.amazonaws.com stratus-gds-use1.s3.us-east-1.amazonaws.com use1.platform.illumina.com
Europe (London)	api.euw2.sh.basespace.illumina.com eu-sh-euw2-prod-seq-hub-data-bucket.s3.amazonaws.com eu-sh-euw2-prod-seq-hub-data-bucket.s3.eu-west-2.amazonaws.com euw2.platform.illumina.com euw2.sh.basespace.illumina.com stratus-gds-euw2.s3.amazonaws.com stratus-gds-euw2.s3.eu-west-2.amazonaws.com
Europe (Frankfurt)	api.euc1.sh.basespace.illumina.com euc1.platform.illumina.com euc1.sh.basespace.illumina.com euc1-prd-seq-hub-data-bucket.s3.amazonaws.com euc1-prd-seq-hub-data-bucket.s3.eu-central-1.amazonaws.com stratus-gds-euc1.s3.amazonaws.com stratus-gds-euc1.s3.eu-central-1.amazonaws.com
Singapore	api.aps1.sh.basespace.illumina.com aps1-sh-prd-seq-hub-data-bucket.s3.amazonaws.com aps1-sh-prd-seq-hub-data-bucket.s3.ap-southeast-1.amazonaws.com aps1.platform.illumina.com aps1.sh.basespace.illumina.com stratus-gds-aps1.s3.amazonaws.com stratus-gds-aps1.s3.ap-southeast-1.amazonaws.com

ICA

In addition to the common domain (ica.illumina.com, listed above), ICA pipelines and clients require outbound connections to the following region-specific domains for storage

Region	URL
United States (US)	stratus-gds-use1.s3.us-east-1.amazonaws.com stratus-gds-use1.s3.amazonaws.com
United Kingdom (GB)	stratus-gds-euw2.s3.eu-west-2.amazonaws.com stratus-gds-euw2.s3.amazonaws.com
Germany (EU)	stratus-gds-euc1.s3.eu-central-1.amazonaws.com stratus-gds-euc1.s3.amazonaws.com
Singapore (SG)	stratus-gds-aps1.s3.ap-southeast-1.amazonaws.com stratus-gds-aps1.s3.amazonaws.com

ICM

Illumina Connected Multiomics (ICM) requires access to the following domain, in addition to the common domains listed above.

Region	URL
United States (US)	use1.icm.illumina.com *.use1.icm.illumina.com
United Kingdom (GB)	euw2.icm.illumina.com *.euw2.icm.illumina.com
Germany (EU)	euc1.icm.illumina.com *.euc1.icm.illumina.com
Singapore (SG)	aps1.icm.illumina.com *.aps1.icm.illumina.com

Summary/Checklist to Prepare your Network

1. Allow Common Domains

- ica.illumina.com

- connected.illumina.com

- login.illumina.com

- *.login.illumina.com

2. Allow Region-Specific Domains for:

- BaseSpace Sequence Hub

- ICA

- ICM

3. Verify Proxy/Firewall Rules

- Ensure no secure (HTTPS) connections are blocked or disrupted by network filtering.