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Sample Sheet Templates

Sample Sheet templates for TSO 500 v2.5.x standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use BaseSpace Run Planner.

DRAGEN TSO 500 analysis software is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

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For interactive run planning or to create a sample sheet for ICA Auto-launch, use BaseSpace Run Plannerarrow-up-right to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to Sample Sheet Creation in BaseSpace Run Planner.

Users can visit the section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

Assay
Instrument
Assay Workflow
File

*Lane numbers cannot exceed what is supported by the flow cell in use.

TSO500 + HRD

NovaSeq 6000Dx (in RUO mode)

Standard

file-downloadTSO 500 HRD_NovaSeq6000Dx_fwd_v2 Sample Sheet Template.csv

TSO500 HT

NovaSeq 6000

Standard

file-downloadTSO 500 HT_NovaSeq6000_SP_S1_S2_S4_v2 Sample Sheet Template.csv

TSO500 HT

NovaSeq 6000

XP*

file-downloadTSO 500 HT_NovaSeq6000_SP_S1_S2_S4_XP_v2 Sample Sheet Template.csv

TSO500 HT

NovaSeq 6000Dx (in RUO mode)

Standard

file-downloadTSO 500 HT_NovaSeq6000DxRUO_S1_S2_S4_v2 Sample Sheet Template_200037181.csv

TSO500 HT

NovaSeq 6000Dx (in RUO mode)

XP*

file-downloadTSO 500 HT_NovaSeq6000DxRUO_S1_S2_S4_XP_v2 Sample Sheet Template_200037182.csv

TSO500

NextSeq 550

Standard

TSO500 + HRD

NextSeq 550

Standard

TSO500 + HRD

NovaSeq 6000

Standard

Sample Sheet guidelines
file-downloadTSO 500 NextSeq550_v2 Sample Sheet Template.csv
file-downloadTSO 500 NextSeq550_v2 Sample Sheet Template.csv
file-downloadTSO 500 HRD_NovaSeq6000_v2 Sample Sheet Template.csv