# DRAGEN v4.3

- [Getting Started](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/getting-started.md)
- [DRAGEN Host Software](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-host-software.md)
- [DRAGEN Secondary Analysis](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-host-software/dragen-platform.md)
- [DRAGEN Reference Support](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-reference-support.md)
- [Prepare a Reference Genome](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-reference-support/prepare-a-reference-genome.md)
- [DRAGEN DNA Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline.md)
- [DNA Mapping](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/dna-map-align.md)
- [Read Trimming](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/read-trimming.md)
- [DRAGEN FASTQC](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/dragen-fastqc.md)
- [Sorting and Duplicate Marking](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/sort-dupmark.md)
- [Small Variant Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling.md)
- [ROH Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/roh-caller.md)
- [B-Allele Frequency Output](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/baf-output.md)
- [Somatic Mode](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/somatic-mode.md)
- [Joint Analysis](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/joint-analysis.md)
- [De Novo Small Variant Filtering](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/filtering.md)
- [Autogenerated MD5SUM for VCF Files](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/small-variant-calling2.md)
- [Force Genotyping](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/force-genotyping.md)
- [Machine Learning for Variant Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/ml-for-vc.md)
- [Evidence BAM](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/evidence-bam.md)
- [Mosaic Detection](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/mosaic-detection.md)
- [VCF Imputation](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/vcf-imputation.md)
- [Multi-Region Joint Detection](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/small-variant-calling/multi-region-joint-detection.md)
- [Copy Number Variant Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling.md)
- [CNV Output](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/cnv-output.md)
- [CNV with SV Support](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/cnv-with-sv-support.md)
- [Multisample CNV Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/multisample-cnv-calling.md)
- [Somatic CNV Calling WGS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/somatic-cnv-calling-wgs.md)
- [Somatic CNV Calling WES](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/somatic-cnv-calling-wes.md)
- [Allele Specific CNV for Somatic WES CNV](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/cnv-calling/somatic-cnv-calling-wes-ascn.md)
- [Repeat Expansion Detection](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/repeat-expansions.md)
- [De Novo Repeat Expansion Detection](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/repeat-expansions/de-novo-str-detection.md)
- [Targeted Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller.md)
- [CYPDB6 Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/cyp2b6-calling.md)
- [CYP2D6 Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/cyp2d6-calling.md)
- [CYP21A2 Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/cyp21a2-calling.md)
- [GBA Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/gba-calling.md)
- [HBA Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/hba-calling.md)
- [LPA Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/lpa-calling.md)
- [Rh Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/rh-calling.md)
- [SMN Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/targeted-caller/smn-calling.md)
- [Structural Variant Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/sv-calling.md)
- [Structural Variant De Novo Quality Scoring](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/sv-calling/sv-denovo-quality-scoring.md)
- [VNTR Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/vntr-calling.md)
- [Filter Duplicate Variants](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/variant-deduplication.md)
- [Ploidy Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/ploidy-calling.md)
- [Ploidy Estimator](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/ploidy-calling/ploidy-estimator.md)
- [Ploidy Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/ploidy-calling/ploidy-caller.md)
- [Multi Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/multi-caller.md)
- [QC Metrics Reporting](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/qc-metrics-reporting.md)
- [HLA Typing](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/hla-typing.md)
- [Biomarkers](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers.md)
- [Tumor Mutational Burden](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers/biomarker-tmb.md)
- [Microsatellite Instability](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers/biomarker-msi.md)
- [Homologous Recombination Deficiency](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers/biomarker-hrd.md)
- [BRCA Large Genomic Rearrangment](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers/biomarker-brca-large-rearrangement.md)
- [DRAGEN Fragmentomics](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/biomarkers/biomarker-fragmentomics.md)
- [Downsampling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/downsampling.md)
- [Fractional (Raw Reads) Downsampling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/downsampling/fractional-downsampler.md)
- [Effective Coverage Downsampling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/downsampling/downsampling.md)
- [Unique Molecular Identifiers](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/unique-molecular-identifiers.md)
- [Indel Re-aligner (Beta)](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/indel-realigner.md)
- [Star Allele Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/star-allele-caller.md)
- [High Coverage Analysis](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/high-coverage.md)
- [CheckFingerprint](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/checkfingerprint.md)
- [Population Haplotyping (Beta)](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/population-haplotyping.md)
- [DUX4 Rearrangement Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-dna-pipeline/dux4-rearrangement-caller.md)
- [DRAGEN RNA Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline.md)
- [RNA Alignment](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline/rna-alignment.md)
- [Gene Fusion Detection](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline/gene-fusion-detection.md)
- [Gene Expression Quantification](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline/gene-expression-quantification.md)
- [RNA Variant Calling](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline/rna-variant-calling.md)
- [Splice Variant Caller](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-rna-pipeline/splice-variant-caller.md)
- [DRAGEN Single-Cell Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-single-cell-pipeline.md)
- [scRNA](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-single-cell-pipeline/dragen-scrna.md)
- [scATAC](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-single-cell-pipeline/dragen-scatac.md)
- [Single-Cell Multiomics](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-single-cell-pipeline/dragen-multiomics.md)
- [DRAGEN Methylation Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-methylation-pipeline.md)
- [DRAGEN Amplicon Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-amplicon-pipeline.md)
- [Explify Analysis Pipeline](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/pipeline.md)
- [Kmer Classifier](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/pipeline/kmer-classifier.md)
- [Kmer Classifier Database Builder](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/pipeline/kmer-class-db-builder.md)
- [DRAGEN Recipes](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes.md)
- [DNA Germline Panel UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-panel-umi.md)
- [DNA Germline Panel](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-panel.md)
- [DNA Germline WES UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-wes-umi.md)
- [DNA Germline WES](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-wes.md)
- [DNA Germline WGS UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-wgs-umi.md)
- [DNA Germline WGS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-germline-wgs.md)
- [DNA Somatic Tumor-Normal Solid Panel UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-panel-umi.md)
- [DNA Somatic Tumor-Normal Solid Panel](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-panel.md)
- [DNA Somatic Tumor-Normal Solid WES UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-wes-umi.md)
- [DNA Somatic Tumor-Normal Solid WES](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-wes.md)
- [DNA Somatic Tumor-Normal Solid WGS UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-wgs-umi.md)
- [DNA Somatic Tumor-Normal Solid WGS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-normal-solid-wgs.md)
- [DNA Somatic Tumor-Only Heme WGS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-heme-wgs.md)
- [DNA Somatic Tumor-Only Solid Panel UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-panel-umi.md)
- [DNA Somatic Tumor-Only Solid Panel](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-panel.md)
- [DNA Somatic Tumor-Only Solid WES UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-wes-umi.md)
- [DNA Somatic Tumor-Only Solid WES](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-wes.md)
- [DNA Somatic Tumor-Only Solid WGS UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-wgs-umi.md)
- [DNA Somatic Tumor-Only Solid WGS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-solid-wgs.md)
- [DNA Somatic Tumor-Only ctDNA Panel UMI](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/dna-somatic-tumor-only-ctdna-panel-umi.md)
- [RNA Panel](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/rna-panel.md)
- [RNA WTS](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-recipes/rna-wts.md)
- [BCL conversion](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/bcl-conversion.md)
- [Illumina Connected Annotations](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/nirvana.md)
- [ORA Compression](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/ora-compression.md)
- [Command Line Options](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/command-line-options.md)
- [DRAGEN Reports](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/dragen-reports.md)
- [Tools and Utilities](https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3/tools-and-utilities.md)


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://help.connected.illumina.com/dragen/dragen-v4.3/product-guide/dragen-v4.3.md?ask=<question>
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The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
