5-base Methylation

Currently, Illumina 5-base supports only PCR prep

The epigenetic methylation of cytosine bases in DNA can have a dramatic effect on gene expression. Bisulfite sequencing is the most canonical method for detecting epigenetic methylation patterns at single-base resolution through converting all unmethylated cytosines to thymine. These libraries inform methylation but have low base diversity which precludes accurate genomic interrogations. Novel Illumina 5-base assay converts methylated cytosine to thymine, retaining high complexity, and is capable of simultaneous variant calling and DNA methylation levels detection. For more information refer to DRAGEN 5-base manual

DRAGEN: DRAGEN 4.5.4 | Truthsets: Methylated pUC19 and unmethylated λ-phage

Illumina 5-base accurately and specifically converts methylated control DNA.

Method	Unmethylated λ	Methylated pUC19
BiSulfite	0.5%	95.0%
EM-Seq	0.4%	96.4%
5-base	0.9%	94.7%

Methylation controls comparison