# Sequencing information

## **Select a coverage BED**

<div align="left"><figure><img src="/files/JcyTHDXAY2lXURafc5rh" alt="" width="563"><figcaption></figcaption></figure></div>

A coverage BED[^1] file is used to calculate and determine quality control (QC) metrics for your case. This file defines the genomic regions that should meet coverage requirements during sequencing.

{% hint style="info" %}
BED files defining custom kits can be added in Organization settings > [Kit management](/emedgene/emedgene-analyze-manual/settings/organization_settings_-330+/kit-management.md).\
Furthermore, the BED file chosen here is linked to a **PON (Panel of Normals) file** when starting from FASTQs and conducting CNV calling.
{% endhint %}

After selecting a coverage BED file, the available reference sequences for this kit will be displayed.

## **Specify sample preparation details**

Specify details such as laboratory name, sequencing machine used, sequencing reagent kit, and expected coverage.

[^1]: Browser Extensible Data


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