> For the complete documentation index, see [llms.txt](https://help.connected.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.connected.illumina.com/emedgene/emedgene-analyze-manual/settings/organization_settings_-330+/workbench-and-pipeline/gnomad-annotation-v100.41+.md).

# gnomAD Annotation (v100.41+)

GRCh37 cases use gnomAD v2.1 for population data annotation, while GRCh38 cases use gnomAD v4.1, available as Non-UKB and All datasets.

Use the **gnomAD Annotation** card to select the gnomAD v4.1 population dataset used for SNV annotation in GRCh38 cases.

## gnomAD v4.1 annotation datasets

The selected dataset annotates SNVs with **Total AF**, **Allele count**, **Hom/Hemi count**, **Max AF**.

The metrics are available on the [**Variant page**](/emedgene/emedgene-analyze-manual/variant_page/variant_page.md), as [**Variant table**](https://help.connected.illumina.com/emedgene/emedgene-analyze-manual/settings/organization_settings_-330+/workbench-and-pipeline/pages/vpEMCVyDac5LYh1zxE6s#id-5.-population-frequency-data) columns, in [**Polymorphism filters**](/emedgene/emedgene-analyze-manual/reviewing_a_case/analysis-tools-tab-beta-v100.39.0+/filters_presets_panel/filters/simple-filter-operators-mode/polymorphism_filters.md), [**Query builder**](/emedgene/emedgene-analyze-manual/reviewing_a_case/analysis-tools-tab-beta-v100.39.0+/filters_presets_panel/filters/advanced-filter-operators-mode-v100.40.0+.md), and in [exports](/emedgene/emedgene-analyze-manual/reviewing_a_case/analysis-tools-tab-beta-v100.39.0+/variant_table/download_variants.md).

**gnomAD 4.1 Non-UKB**

* Excludes UK Biobank samples, helping reduce population bias.
* This is the default option.

**gnomAD 4.1 All**

* Includes UK Biobank samples, providing a larger sample size for allele-frequency calculations.
* Annotation with the **gnomAD 4.1 All** dataset includes the additional **gnomAD Grpmax FAF (95% CI)** metric on the [**Variant page**](/emedgene/emedgene-analyze-manual/variant_page/variant_page.md), as a [**Variant table**](https://help.connected.illumina.com/emedgene/emedgene-analyze-manual/settings/organization_settings_-330+/workbench-and-pipeline/pages/vpEMCVyDac5LYh1zxE6s#id-5.-population-frequency-data) column, in [**Query builder**](/emedgene/emedgene-analyze-manual/reviewing_a_case/analysis-tools-tab-beta-v100.39.0+/filters_presets_panel/filters/advanced-filter-operators-mode-v100.40.0+.md), and in [exports](/emedgene/emedgene-analyze-manual/reviewing_a_case/analysis-tools-tab-beta-v100.39.0+/variant_table/download_variants.md).

The **Versions** tab shows the dataset used for each case.

## Change the gnomAD annotation dataset

<figure><img src="https://1131024994-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGCW0DnLlE7QjoZPNmKIi%2Fuploads%2Fgit-blob-3685ad11283fe29310cd5b71e122723789e6fce7%2Fgnomad%20annotation.gif?alt=media" alt=""><figcaption><p>Selecting the gnomAD dataset for small variant annotation in GRCh38 cases.</p></figcaption></figure>

### Before you begin

* Your pipeline version is v100.41 or later.
* You have the required [permissions](/emedgene/emedgene-analyze-manual/settings/user_roles/iam-scopes-emedgene-roles.md).

{% stepper %}
{% step %}
In the **gnomAD Annotation card**, select **gnomAD 4.1 Non-UKB** or **gnomAD 4.1 All**.
{% endstep %}

{% step %}
Select **Save** to confirm your choice.
{% endstep %}
{% endstepper %}

### Result

* Cases run after the change use the new dataset.
* Cases started before the change retain their original annotations until reanalysis.
* The organization activity log records the change.


---

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